Effects and regulation mechanism of different proportions of red and blue light on quality components in tea (Camellia sinensis L.) plant

doi:10.3969/j.issn.1004-1524.2022.10.04

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (10): 2105-2111.DOI: 10.3969/j.issn.1004-1524.2022.10.04

• Crop Science • Previous Articles Next Articles

Effects and regulation mechanism of different proportions of red and blue light on quality components in tea (Camellia sinensis L.) plant

GE Shibei¹(), JIN Didi², YANG Minglai³, WANG Hui³, ZHANG Lan¹, HAN Wenyan¹, LI Xin¹^,^*()

1. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
2. School of Life Sciences, Anhui Agricultual University, Hefei 230036, China
3. Zhejiang Changxin Photoelectric Technology Co., Ltd., Hangzhou 310020, China

Received:2021-12-06 Online:2022-10-25 Published:2022-10-26
Contact: LI Xin

Abstract

Abstract:

Light is one of the important environmental factors in the growth and development of tea plants. In this study, Longjing 43 was treated with different proportions of red and blue light, and the contents of tea quality components and the expression levels of related genes in tea leaves were determined, to explore the effects of light quality on tea quality and its regulation mechanism. It was shown that the contents of free amino acids and tea polyphenols in tea leaves under supplement of pure red light were not significantly changed compared to the control. However, supplement of different proportions of red and blue light, especially with the irradiation ratio of 8∶1 (red light to blue light), significantly (P<0.05) suppressed the biosynthesis of tea polyphenols, increased the contents of free amino acids, and resulted in the reduction of the ratio of polyphenol to amino acid. This study provided theoretical basis and technical support for the use of supplementary light to regulate the quality of tea.

Key words: Camellia sinensis L., red and blue light, quality components, free amino acids, gene expression

CLC Number:

GE Shibei, JIN Didi, YANG Minglai, WANG Hui, ZHANG Lan, HAN Wenyan, LI Xin. Effects and regulation mechanism of different proportions of red and blue light on quality components in tea (Camellia sinensis L.) plant[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2105-2111.

Figures/Tables 6

Table 1 Sequences of primers used for qRT-PCR

基因名称 Gene	正向引物序列 Forward primer sequences(5'→3')	反向引物序列 Reverse primer sequences(5'→3')
CsGAPDH	TTGGCATCGTTGAGGGTCT	CAGTGGGAACACGGAAAGC
CsPAL	GAATGCCGGTCTTATCCACT	CGGTGAACACCTTGTCAAAC
Cs4CL	GGAGGTTATCCTGGACCTCA	GGCAAGCCTTGTAGTGTGAA
CsUFGT	GGCAAGAAGCTAATAGGGTCGTT	TTGTATCATTCGGAAGTGGTGGG
CsANR	GCGAAGTTGATCCTCTCGTC	AACCACATCGTCAAGTGAACA
CsTS1	AATTCCAAGTTGGGCCTTCT	GCTTCAATCCAAGCTTTTCG
CsTS2	CTCATCAACCTCAACCTTTC	TGCTTGAGGGTACATAATGA

Fig.1 Effect of different ratios of red and blue light on polyphenol content of tea leaves Bars marked without the same letters indicated significant difference at P<0.05. The same as below.

Fig.2 Effect of different ratios of red and blue light on free amino acid content of tea leaves

Fig.3 Effect of different ratios of red and blue light on ratio of polyphenol to amino acid of tea leaves

Fig.4 Effect of different ratios of red and blue light on catechin content of tea leaves EGCG,Epigallocatechin gallate;ECG,Epicatechin gallate;EGC,Epigallocatechin;EC,Epicatechin。

Fig.5 Relative expression level of genes related to tea quality under different ratios of red and blue light

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Effects and regulation mechanism of different proportions of red and blue light on quality components in tea (Camellia sinensis L.) plant

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