Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings

doi:10.3969/j.issn.1004-1524.2022.09.09

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (9): 1901-1910.DOI: 10.3969/j.issn.1004-1524.2022.09.09

• Horticultural Science • Previous Articles Next Articles

Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings

YAN Mei(), YAO Yandong, MOU Kaiping, DAN Yuanyuan, LI Weitai, LIAO Weibiao()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2021-03-24 Online:2022-09-25 Published:2022-09-30
Contact: LIAO Weibiao

Abstract

Abstract:

The 15% macrogol 6000 (PEG-6000) was used to investigate the effect of hydrogen gas (H₂) and abscisic acid (ABA) on drought resistance of tomato (Solanum lycopersicum L.) seedlings and their interaction. The results showed that both H₂ and ABA promoted the growth of tomato seedlings under drought stress, and the optimal concentrations of HRW and ABA were 75% and 150 μmol·L^-1, respectively. HRW treatment could significantly increase endogenous ABA content and the related gene expression in tomato seedling leaves under drought stress. Meanwhile, HRW and ABA alone or together significantly decreased malondialdehyde (MDA) content by 34.8%, 32.6% and 37.0%, whereas HRW and ABA significantly increased the superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity and their gene expression in comparison with PEG treatment. Besides, fluridone (FLU), a synthetic inhibitor of ABA, significantly diminished the positive effect of HRW on tomato seedlings growth, showing that ABA was required for H₂-promoted growth of tomato seedlings under drought stress. FLU suppressed the roles of HRW in MDA content of tomato leaves under drought stress. In addition, FLU suppressed the promotive roles of HRW in the activity of SOD, CAT and APX and their gene expression, indicating that ABA was involved in H₂-enhanced the antioxidant enzyme activities and the realted gene expression under drought stress. Together,the resultsed showed that ABA might be involved in H₂-enhanced the drought resistance of tomato seedlings by decreasing the content of MDA and increasing antioxidant enzyme activities and their gene expression.

Key words: drought stress, hydrogen-rich water, tomato, malondialdehyde content, antioxidant enzyme activity, gene expression

CLC Number:

S641.2

YAN Mei, YAO Yandong, MOU Kaiping, DAN Yuanyuan, LI Weitai, LIAO Weibiao. Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings[J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1901-1910.

Figures/Tables 8

Table 1 Primer sequences for quantitative real-time PCR

基因名称 Gene name	序列号 Accession number	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (5'→3')
SlNCED SlSOD	NM-001247526 M37151.1	ACTTGAACACCCTTTGCCGA CCGATGGCGTGGCTGAAGTG	GCCGGTGGGTGTTACCTTTA CTCAGTTGTTGGACGAGCACTTGT
SlCAT	NM_001247898	GCT CCCAGTTAATGCTCCCAAGTG	GTTTGATCCTTGTCGTCCTGCTGA
SlAPX	NM_001247702	GGGAGATCCA GACCAGAGCGTAG	CTGGAGGACAATCATGGACTGTGC
Actin	SGN-U580609	AATGAACTTCGTGTGGCTCCAGAG	ATGGCAGGGGTGTTGAAGGTTTC

Fig.1 Effect of hydrogen-rich water on tomato seedlings biomass under drought stress The bars of the same index with different letters showed the significant difference (P<0.05). The same as below.

Fig.2 Effect of ABA on tomato seedlings biomass under drought stress

Fig.3 Effect of various treatments on tomato seedlings biomass under drought stress

Fig.4 Effect of HRW treatments on ABA content and gene expression of key enzymes in tomato seedlings under drought stress

Fig.5 Effect of various treatments on malondialdehyde content of tomato seedlings under drought stress

Fig.6 Effect of various treatments on antioxidant enzyme activity of tomato seedlings under drought stress

Fig.7 Effect of various treatments on the expression of antioxidant enzyme genes of tomato seedlings under drought stress

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Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings

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