Effects of NaCl stress on seed germination and physiological characteristics of winter oilseed rape

doi:10.3969/j.issn.1004-1524.2023.03.02

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 499-508.DOI: 10.3969/j.issn.1004-1524.2023.03.02

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Effects of NaCl stress on seed germination and physiological characteristics of winter oilseed rape

XU Yaozhao¹^,²(), ZENG Xiucun²^,³^,^*(), WANG Zhenchao¹, DANG Shizhuo¹, LIU Yongjing¹

1. College of Agriculture and Ecological Engineering, Hexi University, Zhangye 734000, Gansu, China
2. Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Zhangye 734000, Gansu, China
3. School of Life Science and Engineering, Hexi University, Zhangye 734000, Gansu, China

Received:2022-05-06 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

In this study, we investigated the salt tolerance of winter oilseed rape cultivars by subjecting Brassica rapa cultivars of Longyou 16, Tianyou 2, Tianyou 4, and Brassica napus cultivars of Ningyou 2 to NaCl stress at concentration of 34, 68, 102, 136, 170 mmol·L^-1. We measured the seed germination indexes and physiological characteristics. In addition, salt tolerance index and salt tolerance critical value were determined by correlation analysis and regression analysis, respectively. The results showed that seed germination rate, radicle length, sprout length, seedling dry weight and fresh weight all showed a decreasing trend with the increase of NaCl concentration, and the decrease of Brassica rapa cultivars of Longyou 16 and Tianyou 2 and Tianyou 4 was less than that of Brassica napus cultivars of Ningyou 2, and the inhibition degree of high salt concentration (170 mmol·L^-1) on radicle was significant. With increasing salt stress concentration, proline content, cell membrane permeability, malondialdehyde (MDA) content and peroxidase (POD) activity of seedlings gradually increased. Correlation analysis showed that seed germination rate was significantly (P<0.01) positively correlated with radicle length, sprout length, seedling fresh weight and seedling dry weight, while germination rate was significantly (P<0.01) negatively correlated with MDA content, cell membrane permeability, proline content and POD activity; MDA content in rapeseed seedlings was significantly (P<0.01) positively correlated with cell membrane permeability and proline content. Regression analysis showed that critical value of salt tolerance for seed germination and growth index of winter rapeseed was 164.87-251.99 mmol·L^-1, and the median lethal concentration and lethal concentration were 316.29-494.95 mmol·L^-1 and 558.57-883.67 mmol·L^-1, respectively. The critical value of germination growth index of winter rape showed germination rate>sprout length>fresh weight of seedlings>dry weight of seedlings>radicle length. The salt tolerance of winter oilseed rape showed Longyou 16>Tianyou 2>Tianyou 4>Ningyou 2. The salt injury index of winter oilseed rape showed Ningyou 2>Tianyou 4>Tianyou 2>Longyou 16. In conclusion, seed germination rate, radicle length and MDA content of winter oilseed rape could be used as indicators of salt tolerance. These results could provide references for germplasm innovation of salt tolerance winter oilseed rape.

Key words: NaCl stress, winter rapeseed, seed germination, physiological characteristic, salt tolerance critical value, salt tolerance index

CLC Number:

S565.4

XU Yaozhao, ZENG Xiucun, WANG Zhenchao, DANG Shizhuo, LIU Yongjing. Effects of NaCl stress on seed germination and physiological characteristics of winter oilseed rape[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 499-508.

Figures/Tables 8

References 36

[1]	YEO A. Predicting the interaction between the effects of salinity and climate change on crop plants[J]. Scientia Horticulturae, 1998, 78(1/2/3/4): 159-174. DOI URL
[2]	黄昌勇. 土壤学[M]. 北京: 中国农业出版社, 2000.
[3]	何映红. 基于多源遥感数据的漓江流域地表覆盖分类研究[D]. 西安: 长安大学, 2014.
	HE Y H. LULC (land use land cover) research based on multi-source remote sensing data of Lijiang River Basin[D]. Xi’an: Chang’an University, 2014. (in Chinese with English abstract)
[4]	云雪雪, 陈雨生. 国际盐碱地开发动态及其对我国的启示[J]. 国土与自然资源研究, 2020(1): 84-87.
	YUN X X, CHEN Y S. International development of saline-alkali land and its enlightenment to China[J]. Territory & Natural Resources Study, 2020(1): 84-87. (in Chinese with English abstract)
[5]	吴运荣, 林宏伟, 莫肖蓉. 植物抗盐分子机制及作物遗传改良耐盐性的研究进展[J]. 植物生理学报, 2014, 50(11): 1621-1629.
	WU Y R, LIN H W, MO X R. Research progress in the mechanism of plant salt tolerance and genetic engineering of salt resistant crops[J]. Plant Physiology Journal, 2014, 50(11): 1621-1629. (in Chinese with English abstract)
[6]	GUPTA B, HUANG B R. Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization[J]. International Journal of Genomics, 2014, 2014: 701596.
[7]	王治江, 刘自刚, 孙万仓, 等. NaCl和Na₂SO₄胁迫对白菜型冬油菜种子萌发的影响及其耐盐性分析[J]. 干旱地区农业研究, 2016, 34(6): 243-252.
	WANG Z J, LIU Z G, SUN W C, et al. Effects of NaCl and Na₂SO₄ stress on germination of winter rapeseed(Brassica rapa L.) and analysis of salt resistance[J]. Agricultural Research in the Arid Areas, 2016, 34(6): 243-252. (in Chinese with English abstract)
[8]	刘成, 冯中朝, 肖唐华, 等. 我国油菜产业发展现状、潜力及对策[J]. 中国油料作物学报, 2019, 41(4): 485-489.
	LIU C, FENG Z C, XIAO T H, et al. Development, potential and adaptation of Chinese rapeseed industry[J]. Chinese Journal of Oil Crop Sciences, 2019, 41(4): 485-489. (in Chinese with English abstract)
[9]	龙卫华. 油菜发芽期耐盐评价、筛选与盐胁迫下根转录组分析[D]. 北京: 中国农业科学院, 2015.
	LONG W H. Germplasm evaluation, screening of the salt tolerance in rapeseed at the germinatin stage and root transcriptome analysis under salt stress[D]. Beijing: Chinese Academy of Agricultural Sciences, 2015. (in Chinese with English abstract)
[10]	孙万仓, 武军艳, 方彦, 等. 北方旱寒区北移冬油菜生长发育特性[J]. 作物学报, 2010, 36(12): 2124-2134. DOI
	SUN W C, WU J Y, FANG Y, et al. Growth and development characteristics of winter rapeseed northern-extended from the cold and arid regions in China[J]. Acta Agronomica Sinica, 2010, 36(12): 2124-2134. (in Chinese with English abstract) DOI URL
[11]	刘自刚, 王志江, 方圆, 等. NaCl胁迫对白菜型冬油菜种子萌发和幼苗生理的影响[J]. 中国油料作物学报, 2017, 39(3): 351-359.
	LIU Z G, WANG Z J, FANG Y, et al. Effect of salt stress on seed germination and seedling physiology of winter rapeseed (Brassica rapa L.)[J]. Chinese Journal of Oil Crop Sciences, 2017, 39(3): 351-359. (in Chinese with English abstract)
[12]	王亦菲, 黄剑华, 陆瑞菊, 等. NaCl处理对高、低芥酸油菜种子萌发和离体茎尖生长的影响[J]. 核农学报, 2008, 22(6): 762-765.
	WANG Y F, HUANG J H, LU R J, et al. Effect of nacl stress on seed germination and stem apexes growth of rapeseed cultivars with high and low-erucic acid[J]. Journal of Nuclear Agricultural Sciences, 2008, 22(6): 762-765. (in Chinese with English abstract)
[13]	郭月, 于艳芳, 龙卫华, 等. 油菜杂交种宁杂21号及其双亲苗期在盐胁迫条件下差异表达基因分析[J]. 中国油料作物学报, 2020, 42(4): 603-612.
	GUO Y, YU Y F, LONG W H, et al. Genes differentially expression in rapeseed hybrid Ningza 21 and its parents at seedling stage under salt tolerance[J]. Chinese Journal of Oil Crop Sciences, 2020, 42(4): 603-612. (in Chinese with English abstract)
[14]	KOFFLER B E, LUSCHIN-EBENGREUTH N, ZECHMANN B. Compartment specific changes of the antioxidative status in Arabidopsis thaliana during salt stress[J]. Journal of Plant Biology, 2015, 58(1): 8-16. DOI URL
[15]	BU Y Y, KOU J, SUN B, et al. Adverse effect of urease on salt stress during seed germination in Arabidopsis thaliana[J]. FEBS Letters, 2015, 589(12): 1308-1313. DOI URL
[16]	HUSSAIN S, MORILLON R, ANJUM M A, et al. Genetic diversity revealed by physiological behavior of citrus genotypes subjected to salt stress[J]. Acta Physiologiae Plantarum, 2015, 37(1): 1740. DOI URL
[17]	LONG W H, ZOU X L, ZHANG X K. Transcriptome analysis of canola (Brassica napus) under salt stress at the germination stage[J]. PLoS One, 2015, 10(2): e0116217.
[18]	乔佩, 卢存福, 李红梅, 等. 盐胁迫对诱变小麦种子萌发及幼苗生理特性的影响[J]. 中国生态农业学报, 2013, 21(6): 720-727.
	QIAO P, LU C F, LI H M, et al. Influence of salt on seed germination and seedling physiological characteristics of mutagenic wheat[J]. Chinese Journal of Eco-Agriculture, 2013, 21(6): 720-727. (in Chinese with English abstract) DOI URL
[19]	刘宝玉, 张文辉, 刘新成, 等. 沙枣和柠条种子萌发期耐盐性研究[J]. 植物研究, 2007, 27(6): 721-728. DOI
	LIU B Y, ZHANG W H, LIU X C, et al. Salt tolerance of Elaeagnus angustifolia L. and Caragana korshinskii Kom. during germination[J]. Bulletin of Botanical Research, 2007, 27(6): 721-728. (in Chinese with English abstract)
[20]	徐慧妮, 王秀峰, 孙旭东, 等. 黄瓜种子萌发对$\mathrm{NO}_{3}^{-}$胁迫的响应及耐盐性评价[J]. 西北植物学报, 2011, 31(2): 325-331.
	XU H N, WANG X F, SUN X D, et al. Response and stress tolerance evaluation of different cucumber cultivars under $\mathrm{NO}_{3}^{-}$ stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(2): 325-331. (in Chinese with English abstract)
[21]	孔祥生, 易现峰. 植物生理学实验技术[M]. 北京: 中国农业出版社, 2008.
[22]	杨发荣, 刘文瑜, 黄杰, 等. 不同藜麦品种对盐胁迫的生理响应及耐盐性评价[J]. 草业学报, 2017, 26(12): 77-88.
	YANG F R, LIU W Y, HUANG J, et al. Physiological responses of different quinoa varieties to salt stress and evaluation of salt tolerance[J]. Acta Prataculturae Sinica, 2017, 26(12): 77-88. (in Chinese with English abstract)
[23]	ZHAO K F, SONG J, FAN H, et al. Growth response to ionic and osmotic stress of NaCl in salt-tolerant and salt-sensitive maize[J]. Journal of Integrative Plant Biology, 2010, 52(5): 468-475. DOI URL
[24]	LI X H, JIANG D M, LI X L, et al. Effects of salinity and desalination on seed germination of six annual weed species[J]. Journal of Forestry Research, 2011, 22(3): 475-479. DOI URL
[25]	斯琴巴特尔, 吴红英. 盐胁迫对玉米种子萌发及幼苗生长的影响[J]. 干旱区资源与环境, 2000, 14(4): 77-81.
	SECHENBATER, WU H Y. Effects of salt stress on seed germination and seedling growth of maize[J]. Journal of Arid Land Resources and Environment, 2000, 14(4): 77-81. (in Chinese)
[26]	孙君艳, 程琴, 李淑梅. 盐胁迫对小麦种子萌发及幼苗生长的影响[J]. 分子植物育种, 2017, 15(6): 2348-2352.
	SUN J Y, CHENG Q, LI S M. Effect of salt stress on seed germination and seedling growth of wheat[J]. Molecular Plant Breeding, 2017, 15(6): 2348-2352. (in Chinese with English abstract)
[27]	张国伟, 路海玲, 张雷, 等. 棉花萌发期和苗期耐盐性评价及耐盐指标筛选[J]. 应用生态学报, 2011, 22(8): 2045-2053.
	ZHANG G W, LU H L, ZHANG L, et al. Salt tolerance evaluation of cotton (Gossypium hirsutum) at its germinating and seedling stages and selection of related indices[J]. Chinese Journal of Applied Ecology, 2011, 22(8): 2045-2053. (in Chinese with English abstract)
[28]	龙卫华, 浦惠明, 陈松, 等. 油菜3个栽培种发芽期耐盐性评价[J]. 植物遗传资源学报, 2014, 15(1): 32-37.
	LONG W H, PU H M, CHEN S, et al. Evaluation for salt tolerance of three cultivated species of rapeseeds at germination stage[J]. Journal of Plant Genetic Resources, 2014, 15(1): 32-37. (in Chinese with English abstract)
[29]	ZHU J K. Salt and drought stress signal transduction in plants[J]. Annual Review of Plant Biology, 2002, 53: 247-273. DOI URL
[30]	MUKHERJEE S, DAVID A, YADAV S, et al. Salt stress-induced seedling growth inhibition coincides with differential distribution of serotonin and melatonin in sunflower seedling roots and cotyledons[J]. Physiologia Plantarum, 2014, 152(4): 714-728. DOI URL
[31]	KIM S W, JEONG H J, JUNG K H. Integrating omics analysis of salt stress-responsive genes in rice[J]. Genes & Genomics, 2015, 37(8): 645-655.
[32]	ALIA M J, BHALU B, MOHANTY P. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants[J]. Current Science, 2002: 525-532.
[33]	郭远, 王文成, 徐颖莹, 等. 植物耐盐评价方法综述[J]. 江苏农业科学, 2017, 45(23): 18-23.
	GUO Y, WANG W C, XU Y Y, et al. Review on evaluation methods of plant salt tolerance[J]. Jiangsu Agricultural Sciences, 2017, 45(23): 18-23. (in Chinese)
[34]	吕晋慧, 任磊, 李艳锋, 等. 不同基因型茶菊对盐胁迫的响应[J]. 植物生态学报, 2013, 37(7): 656-664. DOI
	LÜ J H, REN L, LI Y F, et al. Responses to salt stress among different genotypes of tea Chrysanthemum[J]. Chinese Journal of Plant Ecology, 2013, 37(7): 656-664. (in Chinese with English abstract) DOI URL
[35]	张春兰, 曹帅, 满丽莉, 等. 不同基因型大豆耐盐性筛选与综合鉴定[J]. 内蒙古民族大学学报(自然科学版), 2019, 34(4): 342-349.
	ZHANG C L, CAO S, MAN L L, et al. Screening and comprehensive identification of salt tolerance of soybean genotypes[J]. Journal of Inner Mongolia University for Nationalities(Natural Sciences), 2019, 34(4): 342-349. (in Chinese with English abstract)
[36]	高英波, 张慧, 薛艳芳, 等. 不同夏玉米品种耐盐性综合评价与耐盐品种筛选[J]. 玉米科学, 2020, 28(2): 33-40.
	GAO Y B, ZHANG H, XUE Y F, et al. Comprehensive evaluation of salt tolerance and screening for salt tolerance accessions of different summer maize varieties[J]. Journal of Maize Sciences, 2020, 28(2): 33-40. (in Chinese with English abstract)

NaCl浓度 NaCl concentration/ (mmol·L^-1)	发芽率Germination rate
NaCl浓度 NaCl concentration/ (mmol·L^-1)	陇油16号 Longyou 16	天油2号 Tianyou 2	天油4号 Tianyou 4	宁油2号 Ningyou 2
0(CK)	96.33 a	97.33 a	94.33 a	90.00 a
34	95.00 a	96.00 a	92.00 a	85.67 b
68	90.66 b	93.00 b	86.67 b	84.67 b
102	86.66 c	89.00 c	81.33 c	80.33 c
136	83.00 d	84.00 d	75.67 d	73.33 d
170	81.00 e	79.00 e	72.67 e	63.33 e

NaCl浓度 NaCl concentration/ (mmol·L^-1)	发芽率Germination rate
NaCl浓度 NaCl concentration/ (mmol·L^-1)	陇油16号 Longyou 16	天油2号 Tianyou 2	天油4号 Tianyou 4	宁油2号 Ningyou 2
0(CK)	96.33 a	97.33 a	94.33 a	90.00 a
34	95.00 a	96.00 a	92.00 a	85.67 b
68	90.66 b	93.00 b	86.67 b	84.67 b
102	86.66 c	89.00 c	81.33 c	80.33 c
136	83.00 d	84.00 d	75.67 d	73.33 d
170	81.00 e	79.00 e	72.67 e	63.33 e

NaCl浓度 NaCl concentration/ (mmol·L^-1)	陇油16号 Longyou 16		天油2号 Tianyou 2		天油4号 Tianyou 4		宁油2号 Ningyou 2
NaCl浓度 NaCl concentration/ (mmol·L^-1)	胚根长 Radical length	胚芽长 Sprout length	胚根长 Radical length	胚芽长 Sprout length	胚根长 Radical length	胚芽长 Sprout length	胚根长 Radical length	胚芽长 Sprout length
0(CK)	7.34 a	2.20 a	8.12 a	2.33 a	7.89 a	2.17 a	6.34 a	1.47 a
34	6.85 b	2.13 a	7.90 a	2.23 a	7.29 b	1.90 ab	5.90 a	1.33 a
68	4.25 c	1.80 b	4.86 b	2.13 a	5.34 c	1.66 bc	4.74 b	0.93 b
102	2.02 d	1.57 c	2.95 c	1.87 b	3.42 d	1.40 cd	3.11 c	0.86 b
136	1.32 e	1.30 d	1.81 d	1.50 c	1.63 e	1.00 de	1.40 d	0.63 c
170	0.63 f	1.03 e	0.66 e	1.37 c	0.69 f	0.83 e	0.51 e	0.43 c

NaCl浓度 NaCl concentration/ (mmol·L^-1)	陇油16号 Longyou 16		天油2号 Tianyou 2		天油4号 Tianyou 4		宁油2号 Ningyou 2
NaCl浓度 NaCl concentration/ (mmol·L^-1)	胚根长 Radical length	胚芽长 Sprout length	胚根长 Radical length	胚芽长 Sprout length	胚根长 Radical length	胚芽长 Sprout length	胚根长 Radical length	胚芽长 Sprout length
0(CK)	7.34 a	2.20 a	8.12 a	2.33 a	7.89 a	2.17 a	6.34 a	1.47 a
34	6.85 b	2.13 a	7.90 a	2.23 a	7.29 b	1.90 ab	5.90 a	1.33 a
68	4.25 c	1.80 b	4.86 b	2.13 a	5.34 c	1.66 bc	4.74 b	0.93 b
102	2.02 d	1.57 c	2.95 c	1.87 b	3.42 d	1.40 cd	3.11 c	0.86 b
136	1.32 e	1.30 d	1.81 d	1.50 c	1.63 e	1.00 de	1.40 d	0.63 c
170	0.63 f	1.03 e	0.66 e	1.37 c	0.69 f	0.83 e	0.51 e	0.43 c

NaCl浓度 NaCl concentration/ (mmol·L^-1)	陇油16号Longyou 16		天油2号Tianyou 2		天油4号Tianyou 4		宁油2号Ningyou 2
NaCl浓度 NaCl concentration/ (mmol·L^-1)	鲜重 Fresh weight/g	干重 Dry weight/mg	鲜重 Fresh weight/g	干重 Dry weight/mg	鲜重 Fresh weight/g	干重 Dry weight/mg	鲜重 Fresh weight/g	干重 Dry weight/mg
0(CK)	0.211 a	18.6 a	0.247 a	22.3 a	0.192 a	16.8 a	0.182 a	15.8 a
34	0.185 b	16.6 b	0.226 a	19.7 a	0.180 a	16.0 ab	0.155 b	11.5 b
68	0.163 c	14.2 c	0.189 b	15.0 b	0.163 b	15.3 b	0.151 b	11.1 b
102	0.145 cd	11.9 d	0.180 bc	12.0 b	0.135 c	11.3 c	0.132 c	9.5 c
136	0.134 d	8.2 e	0.156 c	11.1 bc	0.131 c	9.2 d	0.102 d	6.9 d
170	0.095 e	4.9 f	0.128 d	7.7 c	0.084 d	5.1 e	0.087 d	5.0 e

Effects of NaCl stress on seed germination and physiological characteristics of winter oilseed rape

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NaCl浓度 NaCl concentration/ (mmol·L^-1)	陇油16号Longyou 16		天油2号Tianyou 2		天油4号Tianyou 4		宁油2号Ningyou 2
NaCl浓度 NaCl concentration/ (mmol·L^-1)	脯氨酸含量 Proline content/ (μg·g^-1)	细胞膜透性 Membrane permeability /%	脯氨酸含量 Proline content/ (μg·g^-1)	细胞膜透性 Membrane permeability /%	脯氨酸含量 Proline content/ (μg·g^-1)	细胞膜透性 Membrane permeability /%	脯氨酸含量 Proline content/ (μg·g^-1)	细胞膜透性 Membrane permeability /%
0(CK)	17.29 e	59.6 e	39.41 e	60.4 d	15.49 e	57.0 e	21.28 e	65.6 e
34	22.23 e	66.9 d	48.90 d	61.4 d	20.35 e	63.7 d	27.09 d	71.3 d
68	33.80 d	67.5 d	65.81 c	63.0 c	29.88 d	64.9 d	34.96 c	74.8 c
102	46.38 c	69.6 c	70.94 c	73.3 b	48.18 c	70.5 c	45.28 b	81.1 b
136	66.41 b	80.8 b	84.36 b	73.8 b	67.06 b	74.6 b	48.67 b	82.7 ab
170	83.76 a	87.3 a	93.79 a	78.5 a	78.43 a	81.7 a	60.13 a	84.3 a

NaCl浓度 NaCl concentration/ (mmol·L^-1)	陇油16号Longyou 16		天油2号Tianyou 2		天油4号Tianyou 4		宁油2号Ningyou 2
NaCl浓度 NaCl concentration/ (mmol·L^-1)	丙二醛含量 MDA content/ (μmol· g^-1)	过氧化物酶活性 POD activity/ (U·g^-1)	丙二醛含量 MDA content/ (μmol· g^-1)	过氧化物酶活性 POD activity/ (U·g^-1)	丙二醛含量 MDA content/ (μmol· g^-1)	过氧化物酶活性 POD activity/ (U·g^-1)	丙二醛含量 MDA content/ (μmol· g^-1)	过氧化物酶活性 POD activity/ (U·g^-1)
0(CK)	1.52 f	17.6 e	1.27 f	10.8 e	1.66 e	14.5 c	2.21 d	18.4 b
34	1.78 e	19.2 d	1.49 e	12.7 d	1.94 d	16.0 bc	2.48 d	19.5 b
68	2.22 d	21.9 c	2.06 d	15.3 c	2.17 d	18.1 abc	3.07 c	21.1 b
102	2.42 c	25.8 b	2.44 c	19.5 b	2.86 c	19.5 ab	3.51 b	21.9 b
136	3.02 b	27.9 a	2.71 b	20.9 b	3.34 b	20.9 a	3.82 a	25.8 a
171	3.34 a	28.5 a	3.04 a	23.5 a	3.62 a	21.1 a	4.10 a	27.2 a

指标 Index	发芽率 Germination rate	胚根长 Radicle length	胚芽长 Sprout length	幼苗鲜重 Fresh weight	幼苗干重 Dry weight	丙二醛 MDA	细胞膜透性 Membrane permeability	脯氨酸 Proline
胚根长Radicle length	0.85^**
胚芽长Sprout length	0.94^**	0.77^**
幼苗鲜重Fresh weight	0.92^**	0.88^**	0.91^**
幼苗干重Dry weight	0.91^**	0.93^**	0.89^**	0.97^**
丙二醛MDA	-0.96^**	-0.88^**	-0.97^**	-0.94^**	-0.95^**
细胞膜透性Membrane permeability	-0.86^**	-0.89^**	-0.88^**	-0.90^**	-0.94^**	0.93^**
脯氨酸Proline	-0.54^**	-0.80^**	-0.41^*	-0.53^**	-0.65^**	0.56^**	0.66^**
过氧化物酶POD	-0.73^**	-0.85^**	-0.76^**	-0.85^**	-0.86^**	0.79^**	0.854^**	0.47^*

品种 Cultivar	指标 Index	回归方程 Regression equation	R²	耐盐临界值 Salt tolerance critical value/ (mmol·L^-1)	半致死浓度 Median lethal concentration/ (mmol·L^-1)	致死浓度 Lethal concentration/ (mmol·L^-1)
陇油16号	发芽率Germination rate	y=-0.102 9x+100.93	0.978 1	251.99	494.95	883.67
Longyou 16	胚根长Radical length	y=-0.593 7x+101.56	0.946 7	44.74	86.85	154.22
	胚芽长Sprout length	y=-0.326 4x+103.80	0.984 4	88.24	164.83	287.38
	幼苗鲜重Fresh weight of seedlings	y=-0.297 2x+99.15	0.975 9	81.25	165.37	299.96
	幼苗干重Dry weight of seedlings	y=-0.432 8x+103.36	0.987 1	65.53	123.29	215.71
	均值Average value			106.35	207.06	368.19
天油2号	发芽率Germination rate	y=-0.116 4x+102.08	0.963 3	232.65	447.42	791.07
Tianyou 2	胚根长Radical length	y=-0.593 1x+104.34	0.961 2	49.47	91.62	159.06
	胚芽长Sprout length	y=-0.261 1x+103.90	0.954 3	110.69	206.43	359.63
	幼苗鲜重Fresh weight of seedlings	y=-0.274 5x+99.38	0.987 9	88.81	179.88	325.60
	幼苗干重Dry weight of seedlings	y=-0.378 2x+97.87	0.972 0	60.48	126.58	232.35
	均值Average value			108.42	210.39	373.54
天油4号	发芽率Germination rate	y=-0.146 4x+101.30	0.983 1	179.64	350.41	623.63
Tianyou 4	胚根长Radical length	y=-0.579 8x+104.88	0.982 7	51.54	94.65	163.64
	胚芽长Sprout length	y=-0.374 0x+100.69	0.991 9	68.69	135.53	242.49
	幼苗鲜重Fresh weight of seedlings	y=-0.312 4x+103.44	0.947 9	91.04	171.06	299.10
	幼苗干重Dry weight of seedlings	y=-0.413 8x+108.24	0.932 3	80.33	140.74	237.41
	均值Average value			94.25	178.48	313.26
宁油2号	发芽率Germination rate	y=-0.165 1x+102.22	0.921 9	164.87	316.29	558.57
	胚根长Radical length	y=-0.584 9x+107.65	0.975 5	55.82	98.56	166.95
	胚芽长Sprout length	y=-0.418 4x+99.63	0.974 5	58.93	118.73	214.43
	幼苗鲜重Fresh weight of seedlings	y=-0.298 8x+99.61	0.969 2	82.37	166.04	299.91
	幼苗干重Dry weight of seedlings	y=-0.365 7x+94.31	0.957 6	52.80	121.16	230.54
	均值Average value			82.96	164.16	294.08

NaCl浓度 NaCl concentration/(mmol·L^-1)	陇油16号 Longyou 16	天油2号 Tianyou 2	天油4号 Tianyou 4	宁油2号 Ningyou 2	均值 Mean
34	1.38	1.37	2.47	4.81	2.51
68	5.89	4.45	8.12	5.92	6.09
102	10.04	8.56	13.78	10.74	10.78
136	13.84	13.70	19.78	18.52	16.46
170	15.91	18.83	22.96	29.63	21.84

[1]	MA Zhonghua, WU Na, CHEN Juan, ZHAO Cong, YAN Chenghong, LIU Jili. Effects of salt stress and phosphorus supply on physiological characteristics of switchgrass seedlings [J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1205-1216.
[2]	LI Liyan, TAN Haixia, LI Jing, WANG Lianlong, DU Yinghui, XU Zhiwen. Screening of salt-tolerant growth-promoting Bacillus strains and their effect on oat growth under salt stress [J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1268-1276.
[3]	DUAN Yuanyuan, LIU Xiaohong, WU Jiaqi, GUO Xiaoliang, WANG Fanfan, TANG Tao, YOU Jingmao, GUO Jie. Effects of Magnolia officinalis leaf litter decomposition on growth trait and physiological characteristic of Fritillaria hupehensis [J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2358-2367.
[4]	LIU Ru, DONG Changru, ZHANG Yiwen, QU Minghui, ZHANG Wei, SA Haiyang, CHEN Haiyan, YE Wenling, FAN Ting. Growth-promoting characteristics of Aspergillus niger TL-F2 and its effect on seed germination and cadmium content in seedlings of ryegrass under cadmium stress [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 326-334.
[5]	WU Yan, QIAO Xiaoyan, GE Weiqiang, GAO Qinghai. Effects of exogenous melatonin on physiological and biochemical characteristics in female flowers of Trichosanthes kirilowii under high temperature and strong light [J]. , 2020, 32(3): 421-429.
[6]	SUN Dezhi, YANG Hengshan, ZHANG Qingguo, FAN Fu, Suyale qiqige, PENG Jing, HAN Xiaori. Alleviating effect of exogenous nitric oxide donor sodium nitroprusside on tomato seedlings under salt stress [J]. , 2019, 31(8): 1286-1294.
[7]	LI Mingyu, WANG Yan, LIANG Danni, YAO Yani, LAN Jian. Comprehensive evaluation of salt tolerance of 22 alfalfa germplasms at germination stage [J]. , 2019, 31(5): 746-755.
[8]	HAN Guomin, LIU Xi, TANG Meiling, DAI Lingmin. Effects of exogenous melatonin on physiological characteristics of 5BB grape leaves under NaCl stress [J]. , 2019, 31(4): 556-564.
[9]	DING Xiaoxue, WANG Bingliang, HAI Rui, HU Yuqing, MI Yuehua, YE Hongxia. Studies on germination characteristics of Solanum torvum seeds from various sources [J]. , 2019, 31(3): 420-427.
[10]	LI Yang, LIU Kai, WEI Jipeng, ZHANG Lan, LI Xin, HAN Wenyan, LI Qingyun. Effects of various concentrations of EGCG on seed germination and resistance in cucumber under NaCl stress [J]. , 2018, 30(7): 1160-1167.
[11]	WU Jianhua, WU Zhongxia, WANG Yuanhua, FENG Yingna, YAN Zhiming, CAI Shanya, WANG Quanzhi, SUN Ying. Effect of salicylic acid and proline on gene expression profiles in response to salt stress in lettuce [J]. , 2017, 29(9): 1489-1497.
[12]	SHAO Jincai, LIU Yuxia, YANG Jiaxin, XU Xinglong, ZHANG Qixiang, LI Qingwei. Effects of salt stress on germination and seeding of Chimonanthus praecox seeds [J]. , 2017, 29(7): 1139-1143.
[13]	LI Chunlan, YANG Yonghua, YANG Zhenkun, WANG Jinqiu, LIAO Weibiao. Comparison on the drought-resistance of five ornamental crabapple cultivars [J]. , 2017, 29(5): 782-790.
[14]	YAN Daoliang， ZHENG Bingsong. Effects of soaking seeds in trehalose on physiological characteristics of wheat Yangmai19 under salt stress [J]. , 2016, 28(8): 1271-.
[15]	LI Huimin， LU Xiaomin， ZHAO Shihao. Effects of different light qualities on growth， physiological and stomatal characteristic of okra （Abelmoschus esculentus L. ） seedlings [J]. , 2016, 28(6): 966-.