Effects of different cultivation modes on agronomic characters and accumulation of active components of Ganoderma lucidum strains

doi:10.3969/j.issn.1004-1524.20221254

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (9): 2045-2055.DOI: 10.3969/j.issn.1004-1524.20221254

• Crop Science • Previous Articles Next Articles

Effects of different cultivation modes on agronomic characters and accumulation of active components of Ganoderma lucidum strains

LI Xuelong(), LI Chao, LI Yue, LIU Guoli, ZHANG Peng, ZHANG Min()

Institute of Edible Fungi, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China

Received:2022-08-26 Online:2023-09-25 Published:2023-10-09

Abstract

Abstract:

The aim of the study was to explore the effects of wood log cultivation mode and substrate substitute cultivation mode on main agronomic characters and accumulation of active components of different Ganoderma lucidum strains, and to determine the high-quality and efficient cultivation mode of G. lucidum fruiting body. The wood log and substrate (saw dust, wheat bran, gypsum) were used as the cultivation material, and the average mycelial growth rate, mycelial growth vigor, mycelial growth of full bag time and mycelial characteristics of different G. lucidum strains under the two cultivation modes were compared. The main agronomic traits, such as single fruiting body weight, stalk length, stalk diameter, cap diameter, cap thickness and biological efficiency were compared, too. The contents of triterpenoids, sterols, polysaccharides, proteins and 16 kinds of amino acids in fruiting bodies were detected by pharmacopoeia and national standard methods, and the effects of different cultivation modes on the accumulation of main active components in G. lucidum fruiting bodies were compared. There were some differences in average mycelial growth rate, mycelial growth vigor, mycelial growth full time and mycelial characteristics among the six G. lucidum strains tested under the two cultivation modes. The main agronomic traits of G. lucidum strains under different cultivation modes were significantly different, such as single fruiting body weight, stalk length, stalk diameter, cap diameter, cap thickness and biological efficiency. G. lucidum strain CZ22 got the largest fruiting body weight (117.15 g) and the highest biological efficiency (7.54%) under wood log cultivation mode. Strain CZ22 also got the largest fruiting body weight (40.58 g) and the highest biological efficiency (9.02%) under the substrate substitute cultivation mode. There were some differences in the contents of triterpenoids, sterols and polysaccharides in fruiting bodies of the same G. lucidum strain under different cultivation modes. The highest contents of triterpenes and sterols in fruiting bodies was observed in strain CZ27 (1.14%) under the substrate substitute cultivation mode, and the highest content of polysaccharide in fruiting bodies were achieved by strain CZ25 (1.36%) under the substrate substitute cultivation mode. However, the content of protein, total content of 16 amino acids and each amino acid were significantly different. Compared with the wood log cultivation mode, the G. lucidum cultivated by substitute cultivation mode had higher biological efficiency, larger fruiting body yield and higher content of bioactive components, which provides a theoretical basis for efficient cultivation of G. lucidum.

Key words: Ganoderma lucidum, fruiting body, cultivation modes, agronomic traits, bioactive component

CLC Number:

S567.31

LI Xuelong, LI Chao, LI Yue, LIU Guoli, ZHANG Peng, ZHANG Min. Effects of different cultivation modes on agronomic characters and accumulation of active components of Ganoderma lucidum strains[J]. Acta Agriculturae Zhejiangensis, 2023, 35(9): 2045-2055.

Figures/Tables 7

References 24

[1]	黄年来, 林志彬, 陈国良. 中国食药用菌学[M]. 上海: 上海科学技术文献出版社, 2010: 1623-1624.
[2]	RICHTER C, WITTSTEIN K, KIRK P M, et al. An assessment of the taxonomy and chemotaxonomy of Ganoderma[J]. Fungal Diversity, 2015, 71(1): 1-15.
[3]	CÖR D, KNEZ Ž, KNEZ HRNČIČM. Antitumour, antimicrobial, antioxidant and antiacetylcholinesterase effect of Ganoderma lucidum terpenoids and polysaccharides: a review[J]. Molecules (Basel, Switzerland), 2018, 23(3): 649.
[4]	LIANG C Y, TIAN D N, LIU Y Z, et al. Review of the molecular mechanisms of Ganoderma lucidum triterpenoids: Ganoderic acids A, C2, D, F, DM, X and Y[J]. European Journal of Medicinal Chemistry, 2019, 174: 130-141.
[5]	LEWANDOWSKA U, FICHNA J, GORLACH S. Enhancement of anticancer potential of polyphenols by covalent modifications[J]. Biochemical Pharmacology, 2016, 109: 1-13.
[6]	WU F, ZHOU L W, YANG Z L, et al. Resource diversity of Chinese macrofungi: edible, medicinal and poisonous species[J]. Fungal Diversity, 2019, 98(1): 1-76.
[7]	国家药典委员会. 2020年版中华人民共和国药典一部中药[M]. 北京: 中国医药科技出版社, 2020: 188-189.
[8]	温宝庆, 李文庆, 林坤霞, 等. 灵芝化学成分的HPLC-QTOF MS分析[J]. 今日药学, 2019, 29(11): 761-764.
	WEN B Q, LI W Q, LIN K X, et al. Analysis of Ganoderma by hplc-qtof MS[J]. Pharmacy Today, 2019, 29(11): 761-764. (in Chinese with English abstract)
[9]	SHIAO M S. Natural products of the medicinal fungus Ganoderma lucidum: occurrence, biological activities, and pharmacological functions[J]. Chemical Record (New York, N Y), 2003, 3(3): 172-180.
[10]	ZHANG J J, MA K, CHEN H Y, et al. A novel polycyclic meroterpenoid with aldose reductase inhibitory activity from medicinal mushroom Ganoderma leucocontextum[J]. The Journal of Antibiotics, 2017, 70(8): 915-917.
[11]	宋婷婷, 张作法, 范丽军, 等. 不同类型灵芝品种的农艺性状、显微结构以及功能成分的比较[J]. 菌物学报, 2020, 39(1): 34-41.
	SONG T T, ZHANG Z F, FAN L J, et al. Comparison of agronomic characteristics, microstructures and phytochemical content of friuting body and spore of four cultivated varieties of Ganoderma Lingzhi[J]. Mycosystema, 2020, 39(1): 34-41. (in Chinese with English abstract)
[12]	韦会平, 刘正宇, 谭杨梅, 等. 农作物秸秆栽培灵芝试验效果评价[J]. 中国农学通报, 2005, 21(11): 145-147.
	WEI H P, LIU Z Y, TAN Y M, et al. Experimental study on Ganoderma cultivating with corp stalks[J]. Chinese Agricultural Science Bulletin, 2005, 21(11): 145-147. (in Chinese with English abstract)
[13]	谢丽源, 彭卫红, 黄忠乾, 等. 不同栽培基质灵芝与不同灵芝菌株活性差异及相关性分析[J]. 西南农业学报, 2014, 27(1): 325-330.
	XIE L Y, PENG W H, HUANG Z Q, et al. Correlation analysis of activity differences of different substrates and different strains from Ganoderma lucidum[J]. Southwest China Journal of Agricultural Sciences, 2014, 27(1): 325-330. (in Chinese with English abstract)
[14]	崔大练, 谭海霞, 雷景敏, 等. 豆秸棚栽灵芝的研究[J]. 中国林副特产, 2002(1): 25-26.
	CUI D L, TAN H X, LEI J M, et al. A study on cultivation of Ganderma lucidum with bean stalk in the shed[J]. Quarterly of Forest by-Product and Speciality in China, 2002(1): 25-26. (in Chinese with English abstract)
[15]	袁学军, 陈永敢, 陈光宙, 等. 不同栽培方式对灵芝活性成分的影响[J]. 中国食用菌, 2012, 31(5): 18-19.
	YUAN X J, CHEN Y G, CHEN G Z, et al. Effect of the different cultivation methods on the active components of Ganoderma lucidum[J]. Edible Fungi of China, 2012, 31(5): 18-19. (in Chinese with English abstract)
[16]	李晶晶. 灵芝室内人工栽培方法的研究[D]. 武汉: 湖北中医药大学, 2014.
	LI J J. Study on indoor artificial cultivation of Ganoderma lucidum[D]. Wuhan: Hubei University of Chinese Medicine, 2014. (in Chinese with English abstract)
[17]	吴鸿雪, 吕旭聪, 唐庆九, 等. 栽培条件对灵芝子实体中四种重金属含量的影响[J]. 菌物学报, 2020, 39(1): 137-143.
	WU H X, LYU X C, TANG Q J, et al. Effects of cultivation conditions on the content of four heavy metals in Ganoderma Lingzhi fruiting bodies[J]. Mycosystema, 2020, 39(1): 137-143. (in Chinese with English abstract)
[18]	肖自添, 阎轶, 何焕清, 等. 28个灵芝菌株品比研究[J]. 广东农业科学, 2009, 36(9): 58-60.
	XIAO Z T, YAN Y, HE H Q, et al. Compariative study of twenty-eight Ganoderma lucidum strains[J]. Guangdong Agricultural Sciences, 2009, 36(9): 58-60. (in Chinese with English abstract)
[19]	中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局. 食品安全国家标准食品中蛋白质的测定:3.GB 5009.5-2016[S]. 北京: 中国标准出版社, 2016.
[20]	国家卫生和计划生育委员会,国家食品药品监督管理总局. 食品安全国家标准食品中氨基酸的测定: GB 5009.124—2016[S]. 北京: 中国标准出版社, 2017.
[21]	CHEN T Q, WU Y B, WU J G, et al. Efficient extraction technology of antioxidant crude polysaccharides from Ganoderma lucidum(Lingzhi), ultrasonic-circulating extraction integrating with superfine-pulverization[J]. Journal of the Taiwan Institute of Chemical Engineers, 2014, 45(1): 57-62.
[22]	张海娟, 曹隆枢, 叶少青. 段木灵芝优质高产栽培技术[J]. 浙江农业科学, 2004(3):30-32.
	ZHANG H J, CAO L S, YE S Q. Measures of log-cultivated Ganoderma lucidum for high-yielding and quality[J]. Journal of Zhejiang Agricultural Sciences, 2004(3):30-32. (in Chinese)
[23]	邓丽云, 李小霞, 李丽. 赤灵芝栽培技术[J]. 现代农业科技, 2014(1):110.
	DENG L Y, LI X X, LI L. Cultivation techniques of Ganoderma lucidum[J]. Modern Agricultural Science and Technology, 2014(19):110. (in Chinese)
[24]	齐川, 周慧, 斯金平, 等. 不同品种灵芝主要活性成分分析[J]. 中国实验方剂学杂志, 2012, 18(17): 96-100.
	QI C, ZHOU H, SI J P, et al. Analysis of the main active ingredients from different varieties of Ganoderma lucidum[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(17): 96-100. (in Chinese with English abstract)

菌株 Strain	栽培模式 cultivation modes	菌丝平均生长速度 Mycelial growth average rate/(mm·d^-1)	菌丝长势 Mycelial vigor	菌丝长满袋时间 Mycelial growth of full bag time/d	菌丝特征 Mycelial characteristics
CZ11	段木 Wood log	4.17±0.35 b	++	38.20±1.52 a	浅白,浓密、较整齐 Light white, dense and relatively neat
	代料 Substrate substitute	6.17±0.39 a	++	34.10±1.12 a	浅黄,浓密、整齐 Light yellow, dense and neat
CZ12	段木Wood log	3.95±0.41 b	++	39.15±1.80 a	白色,浓密、整齐White, dense, neat
	代料 Substrate substitute	6.36±0.65 a	+++	33.40±1.51 b	浅白,浓密、较整齐 Light white, dense, and relatively neat
CZ22	段木Wood log	4.75±0.55 b	+++	32.10±2.55 a	浅白,较稀疏、整齐Light white, sparse and tidy
	代料 Substrate substitute	7.24±0.51 a	+++	29.30±1.32 a	微黄,浓密、整齐 Slightly yellow, dense and neat
CZ25	段木 Wood log	4.15±0.27 b	+++	37.80±2.10 a	微黄,较浓密、整齐 Slightly yellow, relatively dense and tidy
	代料 Substrate substitute	6.56±0.40 a	+++	32.45±1.85 a	微黄,较浓密、整齐 Slightly yellow, relatively dense and tidy
CZ27	段木 Wood log	3.88±0.38 b	+	42.20±1.95 a	浅白,较浓密、较整齐 Light white, relatively dense, and relatively tidy
	代料 Substrate substitute	5.52±0.45 a	++	38.20±2.11 b	浅白,浓密、较整齐 Light white, dense, and relatively tidy
CZ28	段木 Wood log	4.17±0.30 b	++	36.50±2.84 a	浅白,较稀疏、较整齐 Light white, relatively sparse and relatively tidy
	代料 Substrate substitute	6.01±0.56 a	++	35.50±1.75 a	微黄,较浓密、整齐 Slightly yellow, relatively dense and tidy

菌株 Strain	栽培模式 cultivation modes	菌丝平均生长速度 Mycelial growth average rate/(mm·d^-1)	菌丝长势 Mycelial vigor	菌丝长满袋时间 Mycelial growth of full bag time/d	菌丝特征 Mycelial characteristics
CZ11	段木 Wood log	4.17±0.35 b	++	38.20±1.52 a	浅白,浓密、较整齐 Light white, dense and relatively neat
	代料 Substrate substitute	6.17±0.39 a	++	34.10±1.12 a	浅黄,浓密、整齐 Light yellow, dense and neat
CZ12	段木Wood log	3.95±0.41 b	++	39.15±1.80 a	白色,浓密、整齐White, dense, neat
	代料 Substrate substitute	6.36±0.65 a	+++	33.40±1.51 b	浅白,浓密、较整齐 Light white, dense, and relatively neat
CZ22	段木Wood log	4.75±0.55 b	+++	32.10±2.55 a	浅白,较稀疏、整齐Light white, sparse and tidy
	代料 Substrate substitute	7.24±0.51 a	+++	29.30±1.32 a	微黄,浓密、整齐 Slightly yellow, dense and neat
CZ25	段木 Wood log	4.15±0.27 b	+++	37.80±2.10 a	微黄,较浓密、整齐 Slightly yellow, relatively dense and tidy
	代料 Substrate substitute	6.56±0.40 a	+++	32.45±1.85 a	微黄,较浓密、整齐 Slightly yellow, relatively dense and tidy
CZ27	段木 Wood log	3.88±0.38 b	+	42.20±1.95 a	浅白,较浓密、较整齐 Light white, relatively dense, and relatively tidy
	代料 Substrate substitute	5.52±0.45 a	++	38.20±2.11 b	浅白,浓密、较整齐 Light white, dense, and relatively tidy
CZ28	段木 Wood log	4.17±0.30 b	++	36.50±2.84 a	浅白,较稀疏、较整齐 Light white, relatively sparse and relatively tidy
	代料 Substrate substitute	6.01±0.56 a	++	35.50±1.75 a	微黄,较浓密、整齐 Slightly yellow, relatively dense and tidy

菌株 Strain	栽培模式 cultivation modes	子实体质量 Fruiting body weight/g	菌柄长度 Length of stipe/cm	菌柄直径 Diameter of stipe/cm	菌盖直径 Diameter of cap/cm	菌盖厚度 Thickness of cap/cm	生物学效率 Biological efficiency/%
CZ11	段木Wood log	102.12±10.20 a	8.24±0.77 a	2.72±0.45 a	17.86±1.85 a	1.81±0.25 a	6.57±0.40 b
	代料Substrate substitute	35.60±3.36 b	6.23±0.71 b	1.58±0.28 b	11.65±1.55 b	1.35±0.22 b	8.15±0.15 a
CZ12	段木Wood log	94.35±9.22 a	7.86±1.10 a	2.97±0.35 a	15.79±1.36 a	1.57±0.32 a	6.07±0.22 a
	代料Substrate substitute	32.34±4.06 b	5.75±0.57 b	1.65±0.25 b	10.48±1.84 b	1.25±0.19 b	7.32±0.22 a
CZ22	段木Wood log	117.15±13.15 a	9.21±0.94 a	3.05±0.57 a	18.95±2.01 a	2.24±0.34 a	7.54±0.37 a
	代料Substrate substitute	40.58±5.14 b	5.30±0.75 b	1.91±0.36 b	13.25±1.21 b	1.56±0.25 b	9.02±0.17 a
CZ25	段木Wood log	110.44±10.32 a	8.45±0.83 a	3.13±0.24 a	16.23±1.53 a	1.69±0.41 a	7.10±0.46 b
	代料Substrate substitute	38.70±4.59 b	6.01±0.43 b	2.14±0.39 b	12.43±1.40 b	1.44±0.21 a	8.65±0.26 a
CZ27	段木Wood log	90.49±9.28 a	9.16±0.90 a	3.01±0.64 a	15.14±2.15 a	1.75±0.20 a	5.82±0.14 b
	代料Substrate substitute	30.94±3.25 b	6.47±0.86 b	2.15±0.40 b	12.15±2.07 b	1.28±0.17 b	7.06±0.09 a
CZ28	段木Wood log	95.48±5.97 a	8.97±0.74 a	2.95±0.55 a	17.68±1.41 a	1.64±0.37 a	6.14±0.38 b
	代料Substrate substitute	36.44±3.10 b	7.85±0.84 a	1.89±0.37 b	10.39±1.40 b	1.30±0.27 b	8.20±0.11 a

菌株 Strain	栽培模式 cultivation modes	子实体质量 Fruiting body weight/g	菌柄长度 Length of stipe/cm	菌柄直径 Diameter of stipe/cm	菌盖直径 Diameter of cap/cm	菌盖厚度 Thickness of cap/cm	生物学效率 Biological efficiency/%
CZ11	段木Wood log	102.12±10.20 a	8.24±0.77 a	2.72±0.45 a	17.86±1.85 a	1.81±0.25 a	6.57±0.40 b
	代料Substrate substitute	35.60±3.36 b	6.23±0.71 b	1.58±0.28 b	11.65±1.55 b	1.35±0.22 b	8.15±0.15 a
CZ12	段木Wood log	94.35±9.22 a	7.86±1.10 a	2.97±0.35 a	15.79±1.36 a	1.57±0.32 a	6.07±0.22 a
	代料Substrate substitute	32.34±4.06 b	5.75±0.57 b	1.65±0.25 b	10.48±1.84 b	1.25±0.19 b	7.32±0.22 a
CZ22	段木Wood log	117.15±13.15 a	9.21±0.94 a	3.05±0.57 a	18.95±2.01 a	2.24±0.34 a	7.54±0.37 a
	代料Substrate substitute	40.58±5.14 b	5.30±0.75 b	1.91±0.36 b	13.25±1.21 b	1.56±0.25 b	9.02±0.17 a
CZ25	段木Wood log	110.44±10.32 a	8.45±0.83 a	3.13±0.24 a	16.23±1.53 a	1.69±0.41 a	7.10±0.46 b
	代料Substrate substitute	38.70±4.59 b	6.01±0.43 b	2.14±0.39 b	12.43±1.40 b	1.44±0.21 a	8.65±0.26 a
CZ27	段木Wood log	90.49±9.28 a	9.16±0.90 a	3.01±0.64 a	15.14±2.15 a	1.75±0.20 a	5.82±0.14 b
	代料Substrate substitute	30.94±3.25 b	6.47±0.86 b	2.15±0.40 b	12.15±2.07 b	1.28±0.17 b	7.06±0.09 a
CZ28	段木Wood log	95.48±5.97 a	8.97±0.74 a	2.95±0.55 a	17.68±1.41 a	1.64±0.37 a	6.14±0.38 b
	代料Substrate substitute	36.44±3.10 b	7.85±0.84 a	1.89±0.37 b	10.39±1.40 b	1.30±0.27 b	8.20±0.11 a

Effects of different cultivation modes on agronomic characters and accumulation of active components of Ganoderma lucidum strains

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