Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate

doi:10.3969/j.issn.1004-1524.20230757

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (5): 1076-1085.DOI: 10.3969/j.issn.1004-1524.20230757

• Horticultural Science • Previous Articles Next Articles

Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate

HUANG Rong¹(), MENG Qingxin¹, WU Xiaoman¹, XUN Lijie¹, ZHANG Junli¹, DONG Xia², DONG Kun¹^,^*(), GONG Xueyang¹^,^*()

1. Faculty of Animal Science and Technology, Yunnan Agricultural University/Eastern Bee Research Institute, Yunnan Agricultural University/Yunnan Provincial Engineering and Research Center for Sustainable Utilization of Honeybee Resources, Kunming 650201,China
2. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received:2023-06-13 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

Taking the pedicel of Camellia reticulata as the test material, the community structure and diversity of endophytic fungi in the pedicel of Camellia reticulata at 12 and 60 years old were studied by high-throughput sequencing. The analysis results showed that there were differences in OTU and Alpha results of endophytic fungi in the pedicels of Camellia reticulata of two tree ages, and there were significant differences in species diversity and richness. Ascomycota and Basidiomycota were the dominant phyla (the sum of their abundance>90%) in the pedicels of Camellia reticulata of two tree ages, but the dominant populations of the two groups of samples at the level of order and family were very different. Many species (fungi) can only be classified to the level of order, among which unclassified_o_Capnodiales of the O group and unclassified_o_Tremellales of the N group accounted for a large proportion. The function of endophytic fungi was predicted to obtain 11 ecological symbionts, with the highest proportion of unknown. Animal pathogens, plant pathogens, fungal parasites, and various saprophytic bacteria formed a multifunctional nutrient type. The abundance of pathogens, parasites, and saprophytic bacteria in endophytic fungi was greatly affected by tree age. In summary, the diversity and richness of endophytic fungi in the pedicels of Camellia reticulata increased significantly, and the species, abundance, and function of dominant fungi also changed. Therefore, the age factor cannot be ignored when using endophytic fungi.

Key words: Camellia reticulate, tree age, diversity of endophytic fungi, high-throughput sequencing

CLC Number:

S794.4

HUANG Rong, MENG Qingxin, WU Xiaoman, XUN Lijie, ZHANG Junli, DONG Xia, DONG Kun, GONG Xueyang. Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate[J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1076-1085.

Figures/Tables 12

Table 1 Comparison of richness of endophytic fungi in pedicels of Camellia reticulate of two tree ages

样本 Sample	OTU数 Number of OTU	门 Phylum	纲 Class	目 Order	科 Family	属 Genus
O组Group O	1 032	4	22	68	150	240
N组Group N	528	4	21	65	123	185

Fig.1 Venn analysis on OTU level The experimental samples were divided into two groups according to tree age: group N (12 a) and group O (60 a). The same as below.

Fig.2 Rarefaction curves of Sobs index (A) and Shannon index (B) of endophytic fungi detected in pedicels of Camellia reticulate of two tree ages

Table 2 Endophytic fungi diversity indexes in pedicels of Camellia reticulate of two tree ages

样本 Sample	Sobs指数 Sobs index	Shannon指数 Shannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	ACE指数 ACE index	覆盖度 Coverage/%
O组Group O	530.33 ± 53.17 a	3.64 ± 0.29 a	0.08 ± 0.02 b	619.90 ± 87.17 a	373.90 ± 35.85 a	99.82
N组Group N	283.67 ± 45.12 b	2.29 ± 0.37 b	0.20 ± 0.05 a	343.70 ± 38.53 b	617.80 ±38.53 b	99.88

Fig.3 Relative abundance of endophytic fungi at phylum level in pedicels of Camellia reticulate of two tree ages

Fig.4 Relative abundance of endophytic fungi at order level in pedicels of Camellia reticulate of two tree ages

Fig.5 Relative abundance of endophytic fungi at family level in pedicels of Camellia reticulate of two tree ages

Fig.6 Comparison of differences in abundance of endophytic fungi at phylum level in pedicels of Camellia reticulate of two tree ages

Fig.7 Comparison of differences in abundance of endophytic fungi at family level in pedicels of Camellia reticulate of two tree ages

Fig.8 PCoA diagram of endophytic fungi community in pedicels of Camellia reticulate of two tree ages

Fig.9 Clustering tree of endophytic fungi community in pedicels of Camellia reticulate of two tree ages

Fig.10 Function prediction of endophytic fungi from pedicels of Camellia reticulate of two tree ages

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Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate

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