Effect of low-temperature stress on proline metabolism and ascorbic acid-glutathione cycle during germination of three Pueraria lobata germplasm lines

doi:10.3969/j.issn.1004-1524.20230863

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (7): 1558-1568.DOI: 10.3969/j.issn.1004-1524.20230863

• Horticultural Science • Previous Articles Next Articles

Effect of low-temperature stress on proline metabolism and ascorbic acid-glutathione cycle during germination of three Pueraria lobata germplasm lines

ZENG Hongxue(), QU Xinghong^*()

School of Hydraulic Engineering, Zhejiang Tongji Vocational College of Science and Technology, Hangzhou 311231, China

Received:2023-07-11 Online:2024-07-25 Published:2024-08-05

Abstract

Abstract:

To investigate the effects of different temperature on proline metabolism and ascorbic acid-glutathione (AsA-GSH) circulatory system during germination of Pueraria lobata seeds, three lines of P. lobata collected from Australian (AUS), and Jiangsu Province (JS), Hunan Province (HN) in China, were subjected to germination tests by using the paper interval method to study the changes in proline content, key enzymes activities of proline metabolism, and contents of non-enzymatic antioxidants and activities of enzymatic antioxidants in the metabolism of AsA-GSH cycle under different treatments (CK, 25 ℃; LT, 15 ℃; LTI1, 10 ℃; LTI2, seed soaking by 0.1% H₂O₂+10 ℃). The results showed that compared to the CK, low temperature stress increased contents of malondialdehyde (MDA), hydrogen peroxide (H₂O₂) and decreased proline dehydrogenase (ProDH) activity. Application of H₂O₂ increased the contents of proline, ascorbic acid (AsA), dehydro ascorbic acid (DHA), glutathione (GSH), glutathione disulfide (GSSG), and activities of Δ¹-pyrroline-5-carboxylic acid synthase (P5CS), ornithine aminotransferase (OAT), ascorbate peroxidase (APX) and glutathione reductase (GR), hence improved the low-temperature resistance of P. lobata. Based on the membership function analysis, the three P. lobata germplasm lines were ranked as HN>AUS>JS in order of cold-tolerance.

Key words: Pueraria lobata, low temperature stress, proline metabolism

CLC Number:

ZENG Hongxue, QU Xinghong. Effect of low-temperature stress on proline metabolism and ascorbic acid-glutathione cycle during germination of three Pueraria lobata germplasm lines[J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1558-1568.

Figures/Tables 7

Fig.1 Effect of low temperature stress on malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents of three P. lobata germplasms Bars marked without the same uppercase letters indicate significant (P<0.05) difference within germplasms under the same treatment, while bars marked without the same lowercase letters indicate significant (P<0.05) difference within treatments for the same germplasm. The same as below.

Fig.2 Effects of low temperature stress on proline content and key enzymes activity for proline metabolism of three P. lobata germplasms P5CS, Pyrroline-5-carboxylate synthase; OAT, Ornithine aminotransferase; ProDH, Proline dehydrogenase.

Fig.3 Effects of low temperature stress on ascorbic acid (AsA), dehydroascorbic acid (DHA) contents of three P. lobata germplasms AsA/DHA represents the ratio of AsA content to DHA content.

Fig.4 Effects of low temperature stress on glutathione (GSH), glutathione disulfide (GSSG) contents of three P. lobata germplasms GSH/GSSG represents the ratio of GSH content to GSSG content.

Fig.5 Effects of low temperature stress on ascorbate peroxidase (APX) and glutathione reductase (GR) activities of three P. lobata germplasms

Table 1 Correlation analysis of physiological and biochemical characteristics of three P. lobata germplasms under low temperature stress

指标 Indices	指标间的相关系数Correlation coefficient within indices
指标 Indices	H₂O₂	MDA	Pro	P5CS	ProDH	OAT	GSSG	GSH	AsA	DHA	APX	GR	AsA/DHA
MDA	0.836^**
Pro	0.428^**	0.669^**
P5CS	0.693^**	0.724^**	0.365^*
ProDH	-0.460^**	-0.541^**	-0.787^**	-0.273
OAT	0.735^**	0.897^**	0.634^**	0.755^**	-0.491^**
GSSG	0.647^**	0.797^**	0.265	0.648^**	-0.124	0.810^**
GSH	0.672^**	0.802^**	0.470^**	0.696^**	-0.256	0.895^**	0.865^**
AsA	0.759^**	0.816^**	0.328	0.769^**	-0.173	0.825^**	0.876^**	0.931^**
DHA	0.806^**	0.894^**	0.524^**	0.649^**	-0.388^*	0.928^**	0.853^**	0.889^**	0.843^**
APX	0.604^**	0.765^**	0.628^**	0.693^**	-0.411^*	0.912^**	0.716^**	0.898^**	0.777^**	0.833^**
GR	0.604^**	0.765^**	0.628^**	0.693^**	-0.411^*	0.913^**	0.715^**	0.899^**	0.777^**	0.828^**	0.998^**
AsA/DHA	-0.326	-0.403^*	-0.476^**	-0.054	0.465^**	-0.483^**	-0.240	-0.244	-0.041	-0.569^**	-0.398^*	-0.390^*
GSH/GSSG	-0.211	-0.291	0.238	-0.159	-0.143	-0.160	-0.590^**	-0.106	-0.248	-0.259	0.034	0.040	0.066

Table 2 Membership degree among all physiological and biochemical indices of three P. lobata germplasms under low temperature stress

指标 Indices	不同种质的隶属度Member degree of germplasms
指标 Indices	AUS	HN	JS
H₂O₂	0.534	0.502	0.525
MDA	0.469	0.603	0.408
Pro	0.494	0.487	0.421
P5CS	0.527	0.510	0.474
ProDH	0.517	0.532	0.461
OAT	0.462	0.475	0.573
GSSG	0.479	0.482	0.523
GSH	0.566	0.527	0.507
AsA	0.572	0.533	0.468
DHA	0.474	0.462	0.485
APX	0.506	0.483	0.502
GR	0.506	0.483	0.502
AsA/DHA	0.429	0.569	0.524
GSH/GSSG	0.520	0.545	0.461
平均值Average	0.504	0.514	0.488

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Effect of low-temperature stress on proline metabolism and ascorbic acid-glutathione cycle during germination of three Pueraria lobata germplasm lines

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