Biological characterization of black spot pathogens in Rosa chinensis and evaluation of varietal resistance

doi:10.3969/j.issn.1004-1524.20240194

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (5): 1087-1096.DOI: 10.3969/j.issn.1004-1524.20240194

• Plant Protection • Previous Articles Next Articles

Biological characterization of black spot pathogens in Rosa chinensis and evaluation of varietal resistance

WANG Chao¹(), LI Yanjie¹, NIU Yun¹, WEN Lianhao¹, CHEN Jingjing¹, WU Hongzhi², YANG Yuyong³, WU Yandi¹^,^*()

1. Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization, Southwest Research Center for Landscape Architecture Engineering (State Forestry and Grassland Administration), Yunnan International Joint R&D Center for Intergrated Utilization of Ornamental Grass, Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China
2. College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China
3. Kunming Yang Chinese Rose Gardening Co., Ltd., Kunming 650503, China

Received:2024-02-29 Online:2025-05-25 Published:2025-06-11

Abstract

Abstract:

To characterize the biological characteristics of two pathogens of black spot disease of Chinese rose (Rosa chinensis) and to identify the disease resistance of 32 varieties of Chinese rose, two pathogens, Alternaria alternata and Gnomoniopsis rosae, were isolated as the pathogen materials. The mycelial growth rate method was used to characterize the biological properties of the two pathogens, and the disease resistance experiments were carried out on 32 Chinese rose varieties individually using the isolated leaf inoculation method. The results showed that temperature, pH, carbon source, nitrogen source and light condition, as well as culture media affected the mycelial growth of the two pathogens. The optimal growth conditions for A. alternata was as follows: 25 ℃, pH of 6, carbon sources of glucose, nitrogen source of protein peptone, 12 h light-12 h dark or 24 h full light, decoction medium of rose leaves. The optimal growth conditions for G. rosae was as follows: 25 ℃, pH of 6, carbon source of sucrose or glucose, nitrogen source of glycine, 12 h light-12 h dark, decoction medium of rose leaves or PDA (potato dextrose agar) medium. The lethal temperature of mycelium was 55 ℃ (10 min) for both A. alternate and G. rosae. Among the 32 Chinese rose varieties, 12 varieties, including New York and Fulu Comedian, were identified as immunity varieties, with strong resistance and immunity to the two pathogens; while five varieties, including Ivory Fashion and Gypsy Boy, were identified as susceptible or severely susceptible varieties, with poor resistance to the two pathogens. These results could provide reference for the prevention and control of black spot disease and the cultivation of disease-resistant rose varieties in Yunnan Province of China.

Key words: black spot disease of Rosa chinensis, Alternaria alternate, Gnomoniopsis rosae, biological characteristics, disease resistance evaluation

CLC Number:

WANG Chao, LI Yanjie, NIU Yun, WEN Lianhao, CHEN Jingjing, WU Hongzhi, YANG Yuyong, WU Yandi. Biological characterization of black spot pathogens in Rosa chinensis and evaluation of varietal resistance[J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 1087-1096.

Figures/Tables 8

References 38

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θ/℃	菌落直径Colony diameter/cm
θ/℃	链格孢菌 Alternaria alternata	蔷薇拟日规壳菌 Gnomoniopsis rosae
5	0 f	0.30±0.20 e
10	1.92±0.19 e	1.26±0.29 d
15	4.50±0.05 c	2.22±0.42 c
20	5.40±0.28 b	3.12±0.12 b
25	5.96±0.32 a	5.82±0.19 a
30	5.00±0.17 bc	1.74±0.04 cd
35	3.84±0.18 d	0.60±0.03 e
40	2.12±0.31 e	0 e

θ/℃	菌落直径Colony diameter/cm
θ/℃	链格孢菌 Alternaria alternata	蔷薇拟日规壳菌 Gnomoniopsis rosae
5	0 f	0.30±0.20 e
10	1.92±0.19 e	1.26±0.29 d
15	4.50±0.05 c	2.22±0.42 c
20	5.40±0.28 b	3.12±0.12 b
25	5.96±0.32 a	5.82±0.19 a
30	5.00±0.17 bc	1.74±0.04 cd
35	3.84±0.18 d	0.60±0.03 e
40	2.12±0.31 e	0 e

pH	菌落直径Colony diameter/cm
pH	链格孢菌 Alternaria alternata	蔷薇拟日规壳菌 Gnomoniopsis rosae
4	7.36±0.16 a	4.90±0.13 b
5	7.00±0.14 a	5.64±0.31 a
6	8.10±0.17 a	5.72±0.10 a
7	6.16±0.30 b	5.56±0.02 a
8	5.74±0.04 b	5.56±0.07 a
9	4.92±0.17 c	4.16±0.24 c
10	5.80±0.16 b	3.80±0.18 c

pH	菌落直径Colony diameter/cm
pH	链格孢菌 Alternaria alternata	蔷薇拟日规壳菌 Gnomoniopsis rosae
4	7.36±0.16 a	4.90±0.13 b
5	7.00±0.14 a	5.64±0.31 a
6	8.10±0.17 a	5.72±0.10 a
7	6.16±0.30 b	5.56±0.02 a
8	5.74±0.04 b	5.56±0.07 a
9	4.92±0.17 c	4.16±0.24 c
10	5.80±0.16 b	3.80±0.18 c

碳源 Carbon source	菌落直径Colony diameter/cm
碳源 Carbon source	链格孢菌 Alternaria alternata	蔷薇拟日规壳菌 Gnomoniopsis rosae
麦芽糖Maltose	6.10±0.10 c	4.34±0.17 b
淀粉Starch	6.46±0.13 bc	3.96±0.12 cd
蔗糖Sucrose	7.72±0.12 b	5.80±0.18 a
木糖Xylose	3.20±0.12 e	3.88±0.05 cd
葡萄糖Glucose	8.20±0.25 a	5.92±0.10 a
果糖Fructose	5.38±0.15 d	4.00±0.11 bc
无碳源No carbon	3.02±0.02 e	1.60±0.05 d

Biological characterization of black spot pathogens in Rosa chinensis and evaluation of varietal resistance

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氮源 Nitrogen source	菌落直径Colony diameter/cm
氮源 Nitrogen source	链格孢菌 Alternaria alternata	蔷薇拟日规壳菌 Gnomoniopsis rosae
硝酸钠Sodium nitrate	5.04±0.10 d	1.80±0.01 c
蛋白胨Protein peptone	7.60±0.21 a	5.00±0.89 b
硝酸钾Potassium nitrate	7.20±0.20 bc	5.38±0.10 ab
牛肉膏Beef extract	7.40±0.46 b	5.44±0.75 ab
甘氨酸Glycine	5.56±0.37 cd	6.50±0.03 a
无氮源No nitrogen	5.00±0.06 d	1.80±0.03 c

光照条件 Light condition	菌落直径Colony diameter/cm
光照条件 Light condition	链格孢菌 Alternaria alternata	蔷薇拟日规壳菌 Gnomoniopsis rosae
12 h光照-12 h黑暗 12 h light-12 h dark	7.84±0.14 a	6.26±0.02 a
24 h全黑暗 24 h full dark	5.14±0.54 b	4.38±0.11 c
24 h全光照 24 h full light	7.54±0.15 a	5.02±0.07 b

培养基 Culture media	菌落直径Colony diameter/cm
培养基 Culture media	链格孢菌 Alternaria alternata	蔷薇拟日规壳菌 Gnomoniopsis rosae
OMA	6.38±0.12 b	2.84±0.15 b
CMA	5.12±0.06 c	2.90±0.17 b
LB	6.04±0.49 b	2.24±0.08 b
PDA	6.40±0.23 b	6.64±0.42 a
YJ	7.50±0.10 a	6.30±0.09 a

品种 Variety	对链格孢菌的抗性Resistance to Alternaria alternata				对蔷薇拟日规壳菌的抗性Resistance to Gnomoniopsis rosae
品种 Variety	发病率 Incidence/%	病级代表值 Disease level representative values	病情指数 Disease index	抗病性级别 Resistance level	发病率 Incidence/%	病级代表值 Disease level representative values	病情指数 Disease index	抗病性级别 Resistance level
纽约New York	3	0	0	I	2	0	0	I
红龙沙宝石Red Eden	80	4	56	SS	4	0	0	I
北京红Beijinghong	5	0	0	I	60	3	42	S
福禄考美地兰Fulu Comedian	5	0	0	I	5	0	0	I
莉莉贝特Lily Bate	0	0	0	I	2	0	0	I
男爵夫人Baronesse	0	0	0	I	0	0	0	I
萨尔曼莎Salmanasar	10	1	14	MR	5	0	0	I
象牙时尚Ivory Fashion	85	4	57	SS	80	4	56	SS
吉普赛男孩Gypsy Boy	30	2	28	S	50	2	28	S
蝴蝶泉Hudiequan	4	0	0	I	0	0	0	I
单瓣浅粉月月红Single Pink China	0	0	0	I	20	1	14	MR
昔日荣光Reine Victoria	15	1	14	MR	25	1	14	MR
甜蜜马车Sweet Chariot	0	0	0	I	5	0	0	I
藤冰山Iceberg	0	0	0	I	15	1	14	MR
极品红Jipinhong	0	0	0	I	30	2	28	S
墨葵Mokui	25	1	14	MR	20	1	14	MR
桃花征服Taohua Zhengfu	5	0	0	I	5	0	0	I
杏花春雨Xinghua Chunyu	2	0	0	I	5	0	0	I
亚伯拉罕Abraham	5	0	0	I	2	0	0	I
黎明大合唱Liming Dahechang	10	1	14	MR	65	3	42	S
金缨子Laevigata Michx	5	0	0	I	8	1	14	MR
桃花香水Sweet Taohua	56	3	42	S	85	4	57	SS
小叶富宁Parvifolia	8	1	14	MR	65	3	42	S
大花香水Gigantea Coll	82	4	56	SS	78	4	56	SS
粉黛塔Fen Dai Ta	4	0	0	I	45	2	27	S
长梗粉团Changgeng Fentuan	26	2	28	S	20	1	14	MR
小金樱Xiaojinying	0	0	0	I	6	0	0	I
藤小伊甸园Mimi Eden	0	0	0	I	5	0	0	I
重瓣红Chongban Hong	5	0	0	I	18	1	14	MR
淡妆Perle d'Or	75	4	55	SS	68	3	41	S
粉色达芬奇Leonardo da Vinci	0	0	0	I	24	1	13	MR
半重瓣白Banchongban Bai	5	0	0	I	0	0	0	I

[1]	SUN Shanshan, CHEMI Lhamo, LI Qiang, ZENG Nanfang, ZHENG Cheng, ZHANG Baiyu, YAN Qigui. Construction and biological characteristics of a recombinant pseudorabies virus expressing GP5-M of PRRSV NADC30-like virus strain [J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2555-2567.
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[4]	CHEN Runchen, WANG Yining, LIU Xiaowen, WANG Hongyan, DING Qiang, WANG Honglei. Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa [J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2330-2338.
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[6]	LI Xuqing, ZHANG Jingze, ZHANG Ya, WU Genliang. Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province [J]. , 2019, 31(5): 784-789.
[7]	ZHOU Huiming, ZHANG Yanzhen, CHAI Hongmei, YANG Rongqing, TAN Ying, ZHANG Pingping, BAI Yuying, ZHAO Yilian, JIN Yujie. Identification and culture conditions of a wild-type Termitomyces aurantiacus strain from Lincang [J]. , 2019, 31(10): 1655-1662.
[8]	MA Xiaoping, YANG Qiuxia, YU Yan, LI Desheng, WANG Chengdong, LING Shanshan, GU Yu. Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt) [J]. , 2018, 30(8): 1328-1335.
[9]	LEI Xueping, GENG Yi, YU Zehui, ZHENG Liping, CAO Shiqi, HUANG Xiaoli, CHEN Defang, OUYANG Ping, LIU Kairui. Isolation and identification of Elizabethkingia meningoseptica from Chinese spiny frog (Quasipaa spinosa) and pathological lesions of its infection [J]. , 2018, 30(3): 371-377.
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[11]	LI Jing\\|jing， DING Song\\|shuang， LI Yan\\|ping， YUN Fei， YAN Hai\\|tao， WANG Zhi\\|meng， LIU Guo\\|shun*. Effects of biochar and nitrogen fertilizers on dry matter accumulation of flue\\|cured tobacco and soil biological characteristics [J]. , 2016, 28(1): 96-.
[12]	LI Xu\\|qing1， TIAN Zhong\\|ling2， ZHENG Ji\\|rong1， ZHENG Jing\\|wu2. Biological characteristics and fungicide screening of Stemphylium lycopersici causing tomato grey leaf spot [J]. , 2015, 27(11): 1953-.
[13]	TANG Bo， ZHANG Dao\\|hua*， ZHANG Xue\\|hua， CHANG Chen， LIU Guo\\|yang， TANG Ying\\|hua， HUA Tao， HOU Ji\\|bo. Isolation and identification of Japanese encephalitis virus from swine#br# [J]. , 2015, 27(10): 1698-.
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