›› 2011, Vol. 23 ›› Issue (3): 0-513.

• 动物科学 •    

Construction of yeast two-hybrid library of 120\|day old Jinhua pig skeletal muscle

CHEN Zhe;SHEN Yi-fei;WANG Ying;XU Ning-ying*   

  1. College of Animal Science, Zhejiang University, Hangzhou 310029, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-25 Published:2011-05-25

Abstract: The whole-length cDNA library of 120-day old Jinhua pig skeletal muscle was constructed by the SMART technique. The titer of library was 1.6 × 106 cfu, the inserted cDNA fragment's length were between 500-2 250 bp, the recombination rate was 93.75%. The results showed that this library was good enough to be applied for the next step.

Key words: Jinhua pig, skeletal muscle, yeast two-hybrid system, cDNA library