Abstract: Tender stem, leaves, shoot apex ,and stem with axillary bud of Cynanchum auriculatum were used as explants in tissue culture and plant regeneration had been studied. The results indicated that the best explants was stem with axillary bud. The most appropriate medium for the original induction was MS+6-BA 1.0 mg ·L-1+NAA 0.1 mg ·L-1,with the induction rate of 100%. The optimum medium for multiplication of buds was MS+KT 1.5 mg ·L-1+NAA 0.05 mg ·L-1,and the coefficient of multiplication was 5.6. The medium for rooting was 1/2 MS+NAA 0.5 mg ·L-1 and the rooting rate reached 93.3%．

Key words: Cynanchum auriculatum, tissue culture, plant regeneration, callus