Abstract:

Using single factor experiment and orthogonal experiment with six factors and three levels, the submerged culture medium and fermentation conditions for optimization of fermentation of strain ZHLS-03 from Shiraia bambusicola were selected based on high free radical scavenging activity and biomass. The results suggested that the optimum medium components and culture conditions were as follows: K2HPO4 0.3%, KCl 0.15%, MgSO4·7H2O 0.1%, FeSO4·7H2O 0.05%, yeast extract 1.5%, glucose 3%, pH 7.0, culture temperature 30℃. This study will provide a basis for further development and utilization of strain ZHLS-03．

Key words: Shiraia bambusicola P.Henn., mycelium, free radical scavenging rate