Abstract:

In order to establish a rapid propagation system of Eleocharis dulcis cv. Diantou Sanwang by tissue culture, the corm terminal buds of Diantou Sanwang was used as explants, and basic MS medium supplemented with 6BA, NAA, AC and PVP at various concentrations was used. The results showed that the optimal induction medium was MS+6BA 15 mg·L-1. For proliferation of the shoots, MS+6BA 15 mg·L-1+NAA 001 mg·L-1 was the best medium, and the highest proliferation rate reached 11.2. The optimal combination of rooting medium was modified as 1/2 MS+NAA 0.05 mg·L-1+AC 1 000 mg·L-1 or MS+NAA 0.05 mg·L-1+AC 1 000 mg·L-1 on which 100% rooting rate was induced. We also found that it could be better in controlling browning when we added 400 mg·L-1 PVP during subculture, and browning rate decreased from 89.6% to 2.0%, meanwhile, the multiplication coefficient increased from 11.20 to 13.85. In this paper, we also made a detailed study on the tissue culture acclimation and transplanting techniques, and the seedling survival rate was up to 100%.

Key words: Eleocharis dulcis, Diantou Sanwang, tissue culture, browning, acclimation, transplanting technique