Prokaryotic expression and immunogenicity detection of SV40-LT gene

›› 2012, Vol. 24 ›› Issue (2): 0-212.

• 动物科学 •

Prokaryotic expression and immunogenicity detection of SV40-LT gene

FU Yu-zhi;;LI Chuan-feng;CHEN Zong-yan;WANG Chao ;CHEN Tie-qiao;*;LIU Guang-qing;*

1Animal Medical College, Hunan Agricultural University, Changsha 410128, China; 2Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-03-25 Published:2012-03-25

Abstract

Abstract: In order to obtain the purified SV40-large T antigen (SV40-LT) and its specific antibodies, the high antigen index region of SV40-LT antigen was overexpressed in DE3 cells firstly, and then the purified LT antigen was vaccinated into rabbit, and induced specific antibody against SV40-LT. The results of western blot and ELISA showed that the expression products not only had well immunogenicity, but also could induce rabbit to produce higher levels of antibodies (antibody titre 1∶6400).

Key words: SV40-LT gene, prokaryotic expression, antibody, ELISA

FU Yu-zhi;LI Chuan-feng;CHEN Zong-yan;WANG Chao;CHEN Tie-qiao;*;LIU Guang-qing;*. Prokaryotic expression and immunogenicity detection of SV40-LT gene [J]. , 2012, 24(2): 0-212.

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Prokaryotic expression and immunogenicity detection of SV40-LT gene

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