Abstract: The explants of Zantedeschia hybrida were disinfected by 0．1％ HgCl2．The results showed that 12 min was the best sterilization time for annual tuber bud while 16min was the best steirlization time for biennial and three-year-old tuber
bud．The best hormone combination was BA 2．0 mg／L+GA3 0．3 mg／L for MS medium，which could induce a higher proliferation multiple of adventitious buds of5.3．Meanwhile，the adventitious buds grew well．The suitable growing medium for plantlet rooting was 1／2 MS medium+ 0．5 mg／L IBA + 10 g／L sucrose．It was benefit to increase the transplantation survival rate when the plantlets were treated with 3 d outdoor acclimation．When transplanted after 80 d，the survival rate of plantlets was 88％．In this experiment，March 10 was the best transplanting date，on which the survival rate of plantlets achieved 100％．

Key words: Zantedeschia hybrida, tissue culture, rapid propagation