Abstract: Porcine oocytes were co-cultured respectively with porcine ovarian cortex cells （pOCCs） and porcine oviductal epithelial cells （pOECs） during in vitro maturation （IVM）. The effects of pOCCs and pOECs co-culture during IVM on the maturation， fertilization and embryos development in vitro were studied. The results showed that cumulus significantly expanded and matured and MⅡoocytes in the co-culture groups were higher than that in the control group （P<0.05）， whereas there was no significant difference in unmatured GV oocytes between the groups（P>0.05）. In addition， the GSH concentration in the pOCCs group was higher than that in other groups （P<0.05）. After IVF， polyspermy in the co\|culture groups were lower than that in the control group （P<0.05）， and there were no significant difference in both fertility and male pronucleus （MPN） formation between the groups（P>0.05）. In conclusion， the presence of the pOCCs and pOECs during IVM enhanced the maturation quality of the porcine oocytes， reduced the polyspermy， and increased the percentages of blastocyst.

Key words: porcine oviductal epithelial cells （pOECs）, porcine ovarian cortex cells （pOCCs）, in vitro maturation （IVM）, oocyte, porcine