Cloning and sequence analysis of OmpA gene of 11 avian Pasteurella multocida strains

›› 2013, Vol. 25 ›› Issue (5): 0-956.

• 论文 •

Cloning and sequence analysis of OmpA gene of 11 avian Pasteurella multocida strains

GAO Ming-yan;XYU Bu*;GONG Jian-sen;WANG Xin;FAN Jian-hua;LIU Xue-xian

Poultry Institute， Chinese Academy of Agricultural Sciences， Yangzhou 225125， China

Received:1900-01-01 Revised:1900-01-01 Online:2013-09-25 Published:2013-09-25

Abstract

Abstract: According to OmpA gene of Pasteurella multocida published in GenBank， a pair of primers was designed. OmpA genes were amplified from 11 avian Pasteurella multocida strains and 3 capsular-type reference strains （A， B， D）
and then cloned into pMD18-T vector and sequenced. The results showed that the ORFs of OmpA gene were between 1 047 bp to 1 077 bp. The predicted maturation protein composed of 327-332 amino-acid besides 21 amino-acid signal peptide. The relative molecular weight of the maturation protein was between 35.19 to 36.36 kD. The alignment results showed that the nucleotide sequence homologies were 85.3%-100% and the deduced amino acid homologies were 83.1%-100% in comparison with 12 reference OmpA sequences of Pasteurella multocida published in GenBank. The homologies of 6 avain Pasteurella multocida strains isolated from China OmpA sequences were 100%. The result revealed that the OmpA gene of avain Pasteurella multocida strains isolated from China is very conservative.

Key words: avian Pasteurella multocida strains, OmpA gene, sequence analysis, homology

GAO Ming-yan;XYU Bu*;GONG Jian-sen;WANG Xin;FAN Jian-hua;LIU Xue-xian. Cloning and sequence analysis of OmpA gene of 11 avian Pasteurella multocida strains[J]. , 2013, 25(5): 0-956.

[1]	WANG Di, YANG Hanmei, LI Yangqian, JIA Mengting, ZOU Liang, YANG Fan. Multidimensional evaluation of “variety, quality, efficiency and application” of Tartary buckwheat and research progress of high-value utilization of active ingredients [J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1960-1974.
[2]	LUO Hailin, YUAN Lei, YAN Jiahui, GUO Qingyun. Cloning and identification of Pepper cryptic virus 2 in Qinghai Province, China [J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1368-1374.
[3]	FAN Lihong, WANG Zhisheng, ZUO Zhicai, CAI Dongjie, YI Jun, MA Xiaoping, GOU Liping, WANG Wei. Analysis of biological characteristics of Acinetobacter pittii from respiratory tract of beef cattle [J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 300-309.
[4]	LIU Kai, FENG Xiaoyu, MA Hengjia, XIE Nan. Complete sequence and gene organization of mitochondrial genome of Megalobrama terminalis from Qiantang River [J]. , 2020, 32(9): 1591-1608.
[5]	NI Zheng, CHEN Liu, HUA Jionggang, YE Weicheng, YUN Tao, ZHU Yinchu, ZHANG Cun. Detection of leukaemia virus antigen and analysis of GP85 gene sequence in local chicken breeds in Zhejiang Province [J]. , 2020, 32(8): 1357-1362.
[6]	WANG Weike, SONG Jiling, LU Na, YUAN Weidong, YAN Jing, CHEN Guanping. Cloning and expression of the PpFBD1 involved in primordium formation of Pleurotus pulmonarius [J]. , 2020, 32(1): 93-97.
[7]	JIANG Yaoyao, LI Jing, CAI Nianjun, CHEN Jianping, ZHANG Hengmu. Cloning and expression analysis of a fibrillin gene in plant [J]. , 2019, 31(9): 1399-1404.
[8]	DEXI Cuomu, WANG Yin, YANG Zexiao, YAO Xueping, LUO Yan, LIAO Changyu, ZHANG Pengfei, JIANG Dike, XIANG Mingyuan, JIANG Ruijiao, SONG Yong. Epidemiological investigation of porcine epidemic diarrhea virus and sequence analysis of ORF3 and S1 genes in Sichuan from 2016 to 2018 [J]. , 2019, 31(9): 1423-1428.
[9]	YI Keke, YIN Wenqi, ZHOU Yuancheng, JIANG Jinzhen, ZHANG Baiyu, LI Zhongyin, YAN Qigui. Isolation and identification of 4 strains of porcine pseudorabies virus and analysis of main virulence genes [J]. , 2019, 31(9): 1429-1436.
[10]	LIU Jialin, LIU Shili, JIANG Wenping, CHENG Shun, CHI Meili, ZHENG Jianbo, JIA Yongyi, ZHAO Jinliang, YIN Shaowu, GU Zhimin. Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila [J]. , 2019, 31(9): 1461-1470.
[11]	WANG Yuanhong, LU Zhimin, ZHANG Xueqi, LIU Zimin, HU Zihui, YANG Kankan, LIU Hongmei, PAN Ling, PENG Kaisong, LI Yongdong, WANG Yong. Isolation, identification and phylogenetic analysis on gp85 gene of subgroup J avian leukosis virus [J]. , 2019, 31(5): 709-715.
[12]	CHEN Xuan, ZHANG Tianye, YANG Jian, ZHANG Hengmu, CHEN Jianping. Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L. [J]. , 2019, 31(1): 98-103.
[13]	BA Shaobo, SHI Lin, LI Qunjing, LIU Zhengkui, CHEN Lin, WANG Xiaodu, SONG Houhui. Isolation, identification and molecullar epidemiological analysis of 17-ZJ-HZ strain of porcine reproductive and respiratory syndrome virus [J]. , 2018, 30(8): 1303-1311.
[14]	SUN Chaochen, YAN Fei, CHEN Jianping. Function of DnaJ-like proteins in infection of Turnip mosaic virus onto Nicotiana benthamiana [J]. , 2018, 30(12): 2056-2064.
[15]	LIU Shili, JIA Yongyi, JIANG Wenping, CHI Meili, CHENG Shun, ZHAO Jinliang, GU Zhimin, FU Jianjun. Cloning and sequence analysis of growth hormone gene and its flanking region in topmouth culter (Culter alburnus Basilewsky) [J]. , 2017, 29(8): 1281-1289.

Cloning and sequence analysis of OmpA gene of 11 avian Pasteurella multocida strains

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments