Cloning and sequence analysis of OmpA gene of 11 avian Pasteurella multocida strains

›› 2013, Vol. 25 ›› Issue (5): 0-956.

• 论文 •

Cloning and sequence analysis of OmpA gene of 11 avian Pasteurella multocida strains

GAO Ming-yan;XYU Bu*;GONG Jian-sen;WANG Xin;FAN Jian-hua;LIU Xue-xian

Poultry Institute， Chinese Academy of Agricultural Sciences， Yangzhou 225125， China

Received:1900-01-01 Revised:1900-01-01 Online:2013-09-25 Published:2013-09-25

Abstract

Abstract: According to OmpA gene of Pasteurella multocida published in GenBank， a pair of primers was designed. OmpA genes were amplified from 11 avian Pasteurella multocida strains and 3 capsular-type reference strains （A， B， D）
and then cloned into pMD18-T vector and sequenced. The results showed that the ORFs of OmpA gene were between 1 047 bp to 1 077 bp. The predicted maturation protein composed of 327-332 amino-acid besides 21 amino-acid signal peptide. The relative molecular weight of the maturation protein was between 35.19 to 36.36 kD. The alignment results showed that the nucleotide sequence homologies were 85.3%-100% and the deduced amino acid homologies were 83.1%-100% in comparison with 12 reference OmpA sequences of Pasteurella multocida published in GenBank. The homologies of 6 avain Pasteurella multocida strains isolated from China OmpA sequences were 100%. The result revealed that the OmpA gene of avain Pasteurella multocida strains isolated from China is very conservative.

Key words: avian Pasteurella multocida strains, OmpA gene, sequence analysis, homology

GAO Ming-yan;XYU Bu*;GONG Jian-sen;WANG Xin;FAN Jian-hua;LIU Xue-xian. Cloning and sequence analysis of OmpA gene of 11 avian Pasteurella multocida strains[J]. , 2013, 25(5): 0-956.

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Cloning and sequence analysis of OmpA gene of 11 avian Pasteurella multocida strains

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