Sequence cloning and prokaryotic expression of omp31 gene in swine Brucella

›› 2014, Vol. 26 ›› Issue (1): 0-28.

• 动物科学 • Next Articles

Sequence cloning and prokaryotic expression of omp31 gene in swine Brucella

School of Mathematics， Physics and Biological Engineering， Inner Mongolia University of Science and Technology， Baotou 014010， China）

Received:1900-01-01 Revised:1900-01-01 Online:2014-01-25 Published:2014-07-09

Abstract

Abstract: In this paper， the swine Brucella genome was used as the template to amplify the omp31 gene sequence and its sequence characteristics and recombinant expression through E. coli prokaryotic expression system were also invetigated. The results showed the ORF sequence of omp31 gene included 723 bp， and it encoded 240 amino acids. The recombinant protein Omp31 was successfully expressed by E. coli prokaryotic expression system. The recombinant protein was purified by GST\|tag affinity column， and the molucelar was consistent with the expected 553 KD.

Key words: Swine Brucella, omp31 gene, sequence cloning, prokaryotic expression, purification

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Sequence cloning and prokaryotic expression of omp31 gene in swine Brucella

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