Abstract: In order to detect the content of pelargonidin 3Oglucoside in strawberry， a new method using reversed phase HPLC was established. The HPLC system consisted of a Waters 1525 Binary Pump and a Waters 2487 dual λ absorbance detector under the control of Breeze software. The volume of 20 μL was injected onto a symmetry C18 column(5 μm， 46 mm×150 mm， Waters)with a symmetry C18 security guard column (5 μm， 39 mm×20 mm) at a flow rate of 1 mL·min-1. The mobile phase was composed of 10% aqueous formic acid water (A) and acetonitrile (B). A linear gradient was performed as follows: 0-20% B (0-13 min)， 20%-40% B (13-20 min)， 40%-0 B (20-25 min). The column temperature was set at 25℃ and the anthocyanin components were detected at 520 nm. The standard working curve had a good linear relationship in the concentration ranged from 025-20 μg·mL-1， the linear equation was y=71859x+13792 and the related coefficient was 0993. The recoveries were 9829%-10269% and the relatively standard deviation was 303. This method is simple, fast， exact， steady and suitable to detect the content of pelargonidin 3Oglucoside in strawberry and other plants．

Key words: strawberry, anthocyanins, pelargonidin 3Oglucoside, reversed phase HPLC