Previous Articles     Next Articles

Analysis of genetic relationship of 30 Jerusalem artichoke germplasm resources by SRAP markers

  

  1. (Research and Development Center of the Jerusalem artichoke,Qinghai Laboratory of Vegetable Genetics and Physiology, Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016,China)
  • Online:2014-09-25 Published:2014-10-11

Abstract: Genetic diversity was analyzed in 30 accessions of Jerusalem artichoke using SRAP markers. 22 primer combinations were used for SRAP\|PCR amplification of Jerusalem artichoke. 160 DNA fragments were amplified from 30 samples and 114 fragments were polymorphic (percentage of polymorphic bands was 71%), indicating that there were wide differences at DNA level among Jerusalem artichoke resource. The genetic distance indexes of 30 cultivars based on the SRAP data varied from 004 to 042. It suggested that the genetic diversity among resources of Jerusalem artichoke was high. Cluster analysis with UPGMA method showed that the 30 resources tested in this study could be divided into two groups. The second group could be divided into six sub groups. The clustering results were slightly different with traditional morphological classification. The above two methods could be combined to provide a theoretical basis for the study on the Jerusalem artichoke resource relationship.

Key words: Jerusalem artichoke(Helianthus tuberosus L.), SRAP, genetic relationship, cluster analysis