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Classification of Echinochloa species in Chinese paddy fields based on ISSR markers

  

  1. (1 China National Rice Research Institute, Hangzhou 311400, China; 2 College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China)
  • Online:2014-09-25 Published:2014-10-11

Abstract: Fifty three Chinese Echinochloa samples collected from 14 provinces were classified through inter\|simple sequence repeat (ISSR) markers. It was shown that 137 bands were amplified by 9 selected primers, and 136 of them were polymorphic. Based on the results, 53 samples were clustered into 9 groups, i.e. E. caudata(5 samples), E. crusgalli var. praticola (8 samples), E. crusgalli var. breviseta(6 samples), E. cruspavonis(3 samples), E. crusgalli var. zelayensis(5 samples), E. crusgalli and E. crusgalli var. mitis(14 samples), E. colona(2 samples), E. glabrescens(5 samples) and E. oryzoides(5 samples). The present classification of Echinochloa species in Chinese paddy fields was in accordance with morphological traits. However, their genetic relationship and systematic level still needs to be further investigated.

Key words: Echinochloa, ISSR molecular marker, cluster analysis