A high\|throughput method for determination of apparent amylase content in rice

A high\|throughput method for determination of apparent amylase content in rice

（1 College of Life Science， Anhui Agricultural University， Hefei 230036， China;2 Institute of Virology and Biotechnology， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021，China）

Online:2014-11-25 Published:2014-12-02

Abstract

Abstract: A rapid and simplified method was developed to determine apparent amylose content in rice based on the standard method （NY/T 83—1988） in this study. A significant positive correlation was found between the modified method and standard method. In addition， the modified method was simple, and less sample and time were needed. The differences in I2\|KI staining could be easily distinguished by eyes （without spectrophotometer） when the difference of apparent amylose content between two samples was above 5%. This modified method could be applied in high throughput screening of the breeding populations with little amount of sample in the future.

Key words: rice, apparent amylose content, high throughput, modified method

DAI Ji\|song1，2， ZHANG Hua2， ZHU Ying2， XU Heng2，*. A high\|throughput method for determination of apparent amylase content in rice [J]. .

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A high\|throughput method for determination of apparent amylase content in rice

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