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Identification of indica\|japonica attribute and prediction of heterosis using InDel markers in rice#br#

  

  1. (1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China;2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)
  • Online:2015-08-25 Published:2015-08-21

Abstract: In this paper, indica\|japonica attribute of rice varieties including Zhejing 4A, 22 restorer lines and their combinations were analyzed by 21 pairs of insertion/deletion (InDel) molecular markers. The relationship between the parents genetic distances and heterosis was studied based on production traits of the combinations, and the feasibility for using InDel markers genetic distance to predict heterosis was also analyzed. Indica\|japonica attribute of each rice variety was identified by NTSYS clustering analysis with the polymorphism of the materials on the InDel site, and the results was basically identical with Wang Ming\|juns. The results showed that genetic distance had significantly positive correlation with total grains per panicle, grains per panicle, grain weight per plant, and the coefficients (R2) were 0627**, 0602**, 0712**, respectively, and had positive correlation with panicles per plant (R2=0426*), but showed no significant positive correlation with seed setting rate and thousand seed weight, the coefficients (R2) were 0217 and 0004, respectively. This study indicated that the InDel markers could be used to identify indica\|japonica attribute and to predict the heterosis in rice.

Key words: rice, InDel molecular markers, indica\, japonica identification, heterosis