Abstract: In order to elucidate the molecular mechanism of anthocyanin biosynthesis in strawberry， the DNA and cDNA sequences of MYB10 and MYB1 gene were amplified from seven strawberry varieties （lines）， Akihime， Benihoppe， Toyonaka， Honghua， Yueli， Yuezhu and 10\|26\|77. The promoter sequences of MYB10 and MYB1 gene were also amplified from genomic DNA. The gene expression patterns and anthocyanin contents in different developmental stages of Akihime and 10\|26\|77 were screened using SYBR Green\|Ⅰ real\|time fluorescent quantitative PCR and HPLC， respectively. The results showed that there was no difference in MYB10 and MYB1 genes at cDNA level among strawberry varieties， but there were different SSR sites in DNA sequences. MYB10 played a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening of Fragaria ×ananassa fruits. And the anthocyanin contents were consistent with changes in the MYB10 gene expression in different fruit stages. The difference in promoter of MYB10 was the main cause of difference in gene expression， which led to the difference in the anthocyanin content and fruit color of strawberry cultivars.

Key words: anthocyanin, transcription factor, strawberry