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The development and verification of nucleotide polymorphism markers in rice

  

  1. (1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; 2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China)
  • Online:2016-03-25 Published:2016-04-07

Abstract: In this work, polymorphic analysis among 3 japonica cultivars (NIP, ZH11 and WY7) and 4 indica cultivars (9311, MH63, TN1 and NJ6) were conducted with 203 molecular markers, 92 polymorphism markers between japonica cultivars and indica cultivars, 34 polymorphism markers in japonica cultivars and 80 polymorphism markers in indica cultivars had been screened. Sequencing analysis results showed that the polymorphism of microsatellite markers among different varieties was mainly caused by the different repetitions of repetitive sequence. However, the polymorphism of insertion/deletion marks was determined by the repetition times of nonsimple repetitive sequence and insertion or deletion of long fragment. Besides the development of new markers, several RM markers also had been verified whether possessed polymorphism in different varieties. Taken together, these new polymorphism markers were bound to be of great service to use in germplasm identification, evolution, genetic diversity detection and molecular markerassisted selection.

Key words: rice, molecular markers, polymorphism, cultivar identification