Analysis of mitochondrial CO Ⅰ gene bar code and its upstream tRNA sequence determination

doi:10.3969/j.issn.1004-1524.2017.01.08

›› 2017, Vol. 29 ›› Issue (1): 51-57.DOI: 10.3969/j.issn.1004-1524.2017.01.08

• Animal Science • Previous Articles Next Articles

Analysis of mitochondrial CO Ⅰ gene bar code and its upstream tRNA sequence determination

ZHANG Yang¹, WANG Meiqing¹, MU Chunyu¹, YAO Wencheng¹, YANG Yaozong¹, BIAN Youqing², CHEN Guohong^{1, *}

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Modern Animal Husbandry Science and Technology Park, Taizhou 225300, China

Received:2016-05-30 Online:2017-01-15 Published:2017-02-23

Abstract

Abstract: This study was conducted to find the effective and accurate identification of the DNA bar code in poultry breed (goose), in order to identify poultry breed or the products of goose. In this study, Anser cygnoides, Anser anser, 3 breeds of local Chinese goose and 3 breeds of European goose were used. Gene sequence based on the mitochondrial cytochrome oxidase subunit Ⅰ gene (COⅠ) was conducted by DNA sequence, and the polymorphism, the relationship of molecular system evolution and the upstream region of the tRNA sequences on goose were also determined. The results showed that: 1) There were 17 mutation sites and 20 haplotypes in the sequence of COⅠ(1 551 bp). 2) Eighteen haplotypes were specific to each species, which could be used as the basis for identification. 3) Seven breeds had specific mutation sites except Carlos. Based on this sequence, the phylogenetic tree constructed by the Kiumura double parameter distance can fully reflect the relationship between the classification and evolution of different geese. 4) Sequence analysis found that Anser cygnoides had 22 kinds of mitochondrial tRNA gene, which were the standard of four constant arm of cloverleaf secondary structure, including 2 tRNA-Ser, 2 tRNA-Leu and the rest were 1. This sequence of COⅠ could be used as the basis for the identification of some geese breeds.

Key words: goose, DNA bar code, sequence analysis, tRNA

CLC Number:

S835

ZHANG Yang, WANG Meiqing, MU Chunyu, YAO Wencheng, YANG Yaozong, BIAN Youqing, CHEN Guohong. Analysis of mitochondrial CO Ⅰ gene bar code and its upstream tRNA sequence determination[J]. , 2017, 29(1): 51-57.

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Analysis of mitochondrial CO Ⅰ gene bar code and its upstream tRNA sequence determination

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