Abstract: In order to evaluate the status of microbial contamination in strawberry, hygienical microbiology was examined from strawberry which sampled in greenhouse and market of Zhejiang Province during March to April in 2016 year according to the food hygiene standard of People Republic of China. Then bacteria counting, enumeration of coliforms and mold detection were carried out. The bacteria and mold which were isolated from the samples were identified by Vitek Auto Microbic System and Molecular biological methods, respectively. The results indicated that the number of bacteria, coliforms and mold of strawberry in greenhouse ranged from 120 to 48 600 cfu·g^-1, from 3 to~1 100 MPN·g^-1, from 0 to 2 900 cfu·g^-1, respectively. And the number of bacteria, coliforms and mold of strawberry in market ranged from 1 900 to 36 900 cfu·g^-1, from 15 to 1 100 MPN·g^-1, from 200 to 6 100 cfu·g^-1, respectively. Meanwhile, 19 gram negative bacteria species such as Escherichia coli, Enterobacter cloacae, Enterobacter amnigenus, Raoultella planticola and 5 mold species such as Mucor and Fusarium were identified. Most of the identified bacteria and mold had serious pathogenicity. The experimental results would be helpful to control the number of microorganisms and develop new sterile technology for strawberry.

Key words: strawberry, microorganism, gram negative bacteria, mold