Genetic analysis of S and ORF3 gene in Porcihe epidemic diarrhea virus during 2011—2016 in Zhejiang Province

doi:10.3969/j.issn.1004-1524.2017.03.05

›› 2017, Vol. 29 ›› Issue (3): 380-388.DOI: 10.3969/j.issn.1004-1524.2017.03.05

• Animal Science • Previous Articles Next Articles

Genetic analysis of S and ORF3 gene in Porcihe epidemic diarrhea virus during 2011—2016 in Zhejiang Province

WU Wangxia1^{1, 2}, WANG Yicheng2^{2, *}, WU Run¹, YUAN Xiufang², XU Lihua², LI Junxing², SU Fei²

1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
2. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-08-17 Online:2017-03-20 Published:2017-03-31

Abstract

Abstract: In order to investigate recent genetic variations of S and ORF3 genes in Porcine epidemic diarrhea virus(PEDV) epidemic and vaccine strains in Zhejiang Province, virus RNA was extracted from suspected PEDV samples which were collected from various areas in Zhejiang Province since 2011 to 2016, then RT-PCR, cloning and sequencing on S and ORF3 genes were performed using three pairs of specific primers to analyze the sequence variation, genetic evolution and antigen sites. The results showed that as compared to vaccine strain CV777, 15 nucleotides were inserted and 6 nucleotides were absent in the S genes of 12 epidemic strains, leading to the insertion of 5 amino acids (⁵⁸QGVN⁶¹and¹⁴⁰N) and deletion of 2 amino acids (¹⁶³DI¹⁶⁴) relatively. Moreover, in epidemic strains, totally 8 amino acid mutations were found in two neutralizing epitopes (499-638 and 764-771 aa) on the major mutation region S1; 7 amino acids were variated in ORF3 gene. According to the analysis results of genetic evolution, these 12 epidemic strains shared 97.8%-99.9% homology with China epidemic strains BJ-2011 and HuN in S gene, but only 93.3%-94.9% homology with vaccine strains (CV777 and Korean attenuated strain DR13), indicating that huge variations and fast evolution had emerged in PEDV recently. This study could lay a foundation for development of effective vaccines and therapies.

Key words: Porcine epidemic diarrhea virus, S gene, ORF3 gene, sequence characteristics, genetic evolution analysis

CLC Number:

S858.28

WU Wangxia1, WANG Yicheng2, WU Run, YUAN Xiufang, XU Lihua, LI Junxing, SU Fei. Genetic analysis of S and ORF3 gene in Porcihe epidemic diarrhea virus during 2011—2016 in Zhejiang Province[J]. , 2017, 29(3): 380-388.

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Genetic analysis of S and ORF3 gene in Porcihe epidemic diarrhea virus during 2011—2016 in Zhejiang Province

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