Morphological type and molecular identification of ectomycorrhiza on Pinus sylvestris var. mongolica

doi:10.3969/j.issn.1004-1524.2017.10.12

›› 2017, Vol. 29 ›› Issue (10): 1678-1685.DOI: 10.3969/j.issn.1004-1524.2017.10.12

• Horticultural Science • Previous Articles Next Articles

Morphological type and molecular identification of ectomycorrhiza on Pinus sylvestris var. mongolica

ZHANG Wenquan¹, LUO Guotao¹, YU Yang², YAN Wei^{3, *}

1. Kaili University, Kaili 556011, China;
2. China Institute of Geo-Environment Monitoring, Beijing 100081, China;
3. College of Forestry, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2017-06-14 Online:2017-10-20 Published:2017-12-05

Abstract

Abstract: The primary study on the diversity of ectomycorrhiza associated with Pinus sylvestris var mongolica in Inner Mongolia surrounding area was investigated using morphological, anatomical and molecular method, and the sequences obtained were blasted using GenBank. The results indicated that there were 19 species different ectomycorrhizas on Pinus sylvestris var. mongolica in Inner Mongolia area. Among these 19 species ectomycorrhizas fungi, 3 species belong to Ascomycotina and the rest species belong to Basidiomycotina, they belong respectively to Cortinarius, Inocybe, Suillus, Sebacina, Russula, Lactarius, Tomentella, Hebeloma, Cenococcum, Tuber, Tricholoma. The results showed that the diversity of ectomycorrhizas on Pinus sylvestris var. mongolica was high, and it was different with results identified by fruiting body associated with Pinus sylvestris var. mongolica.

Key words: Pinus sylvestris var. mongolica, ectomycorrhiza, morphological and anatomical characteristics, molecular identification, ITS

CLC Number:

S718.81

ZHANG Wenquan, LUO Guotao, YU Yang, YAN Wei. Morphological type and molecular identification of ectomycorrhiza on Pinus sylvestris var. mongolica[J]. , 2017, 29(10): 1678-1685.

References

[1] SMITH S E, READ D J. Mycorrhizal symbiosis[M]. San Diego: Academic Press, 2008: 23-46.
[2] 赵忠, 马刊欣, 段安安. 毛白杨外生菌根类型及其生态学特性的研究[J]. 林业科学, 1993, 29(1): 12-18.
ZHAO ZH, MA K X, DUAN A A. Research on types and ecological characteristics of ectomycorrhiza of Populus tomentosa [J]. Scientia Silvae Sinicae , 1993, 29(1): 12-18. (in Chinese with English abstract)
[3] 唐明, 陈辉, 郭建林,等. 陕西省杨树外生菌根种类的调查研究[J]. 林业科学, 1994, 30(5): 437-441,482.
TANG M, CHEN H, GUO J L, et al. A study of the ectomycorrhiza of poplar[J]. Scientia Silvae Sinicae , 1994, 30(5): 437-441, 482. (in Chinese with English abstract)
[4] 陈辉, 唐明. 杨树菌根研究进展[J]. 林业科学, 1997, 33(2): 183-188.
CHEN H, TANG M. Advances in mycorrhizal research on poplar[J]. Scientia Silvae Sinicae , 1997, 33(2): 183-188. (in Chinese with English abstract)
[5] 弓明钦, 王凤珍, 陈羽,等. 西南桦对菌根的依赖性及其接种效应研究[J]. 林业科学研究, 2000, 13(1): 8-14.
GONG M Q, WANG F Z, CHEN Y, et al. Mycorrhizal dependency and inoculant effects on the growth of Betula alnoides seedlings[J]. Forest Research , 2000, 13(1): 8-14. (in Chinese with English abstract)
[6] 李桂兰, 麻德业. 白桦幼苗外生菌根的初步研究[J]. 山西农业大学学报(自然科学版), 2002, 22(2): 127-128.
LI G L, MA D Y. Ectomycorrhizal formation in seedlings of B.platyphylla Suk[J]. Journal of Shanxi Agricultural University ( Natural Science Edition ), 2002, 22(2): 127-128. (in Chinese with English abstract)
[7] SIMARD S W, MOLINA R, SMITH J E, et al. Shared compatibility of ectomycorrhizae on Pseudotsuga menziesii and B.[J]. Canadian Journal of Forest Research , 1997, 27(3): 331-342.
[8] JONES M D, DURALL D M, HARNIMAN S M K. Ectomycorrhizal diversity on Betula papyrifera and Pseudotsuga menziesii seedlings grown in the greenhouse or outplanted in single-species and mixed plots in southern British Columbia[J]. Kluwer Academic Publishers ,1997,27(11):1872-1889.
[9] 樊永军. 内蒙古地区四种树木外生菌根形态多样性及分子鉴定[D]. 呼和浩特: 内蒙古农业大学, 2010.
FAN Y J. Diversity analysis and molecular identification of ectomycorrhizae of several dominant tree species in Inner Mongolia Area[D]. Hohhot: Inner Mongolia Agricultural University, 2010.
[10] AGERER R. Characterization of ectomycorrhiza[M]// NORRIS J R, READ D J, VARMA A K. Techniques for the study of mycorrhiza[M]. Amsterdam: Academic Press, 1991.
[11] HAUG I, PRITSCH K. Ectomycorrhizal types of spruee ( Picea abies CL.karst.) in the Blank forest: A microscopical alas[M]. Tubingen: Kernforschungszentrun Karlsruhe, 1992: 71-89.
[12] JAKUCS E, KOVÁCS GM, SZEDLAY G, et al. Morphological and molecular diversity and abundance of tomentelloid ectomycorrhizae in broad-leaved forests of the Hungarian Plain[J]. Mycorrhiza , 2005, 15(6): 459-470.
[13] GARDES M, BRUNS T D. Community structure of ectomycorrhizal fungi in a Pinus muricata , forest: above-and below-ground views[J]. Canadian Journal of Botany , 1996, 74(10): 1572-1583.
[14] GARDES M, BRUNS T D. ITS primers with enhanced specificity for basidiomycetes: application to the identification of mycorrhizae and rusts[J]. Molecular Ecology , 1993, 2(2): 113.
[15] WHITE T J, BRUNS T D, LEE S B, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]// INNIS M A, GELFAND D H, SNINSKY J J. PCR Protocols: a guide to methods and applications. New York: Academic Press Inc., 1990: 315-322.
[16] 樊永军, 闫伟, 王黎元. 贺兰山地区青海云杉外生菌根的形态类型及分子鉴定[J]. 林业科学, 2011, 47(6):108-113.
FAN Y J, YAN W, WANG L Y. Morphological type and molecular identification of ectomycorrhizae on Picea crassifolia in Helan Mountain[J]. Scientia Silvae Sinicae , 2011, 47(6): 108-113. (in Chinese with English abstract)
[17] 樊永军, 闫伟. 内蒙古地区白桦外生菌根形态类型及分子鉴定[J]. 西北植物学报, 2013, 33(11): 2209-2215.
FAN Y J, YAN W. Morphological type and molecular identification of ectomycorrhizae on Betula platyphylla in Inner Mongolia Area[J]. Acta Botanica Boreali-Occidentalia Sinica , 2013, 33(11): 2209-2215. (in Chinese with English abstract)
[18] 弓明钦, 仲崇禄,陈羽, 等. 菌根型食用菌及其半人工栽培[M]. 广州: 广东科技出版社中国林业出版社, 2007.
[19] 郭尚, 周林. 菌根食药用菌研究[M]. 北京: 中国农业科技出版社, 2017.

Morphological type and molecular identification of ectomycorrhiza on Pinus sylvestris var. mongolica

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