Diversity of endophytic fungi in Bletilla striata

doi:10.3969/j.issn.1004-1524.2017.12.16

›› 2017, Vol. 29 ›› Issue (12): 2077-2083.DOI: 10.3969/j.issn.1004-1524.2017.12.16

• Plant Protection • Previous Articles Next Articles

Diversity of endophytic fungi in Bletilla striata

XI Gangjun¹, LI Jingbao², SHI Jun¹, HAN Zhengmin^{2, *}

1. Bio-Engineering Center, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 211121, China;
2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Received:2017-08-21 Online:2017-12-20 Published:2018-01-08

Abstract

Abstract: To research the diversity of endophytic fungi in Bletilla striata, DNA of endophytic fungi was extracted from the fresh root of Bletilla striata, and ITS rDNA library was constructed. Then, this library was analyzed by high-throughput Illumina sequencing technology and bioinformatics. The results showed that endophytic fungi of Bletilla striata were mainly distributed in Basidiomycota (83.4%) and Ascomycota (14.3%), the other 2.3% could't be identified. Dominance index of endophytic fungi were Basidiomycota (0.834 1), Ascomycota (0.142 6), Zygomycota (0.000 3), and unidentified (0.022 9). At Class and Order levels, the proportion of (Agaricomycetes) Sebacinales was 83.4%, (Discomycete) Pezizales was 11.4%, (Leotiomycetes) Helotiales was 2.1%, unidentified was 2.3%. At Family level, Serendipitacea was 83.4% and Pezizaceae was 11.4%. At Genus level, Piriformospora was 83.4%. Analysis of Shannon index, Chao1, Observed species and Simpson showed that diversity of endophytic fungi in Bletilla striata were uniformly distributed.

Key words: Bletilla striata, endophytic fungi, high-throughput sequencing, classification level, diversity

CLC Number:

S567.23⁺9

XI Gangjun, LI Jingbao, SHI Jun, HAN Zhengmin. Diversity of endophytic fungi in Bletilla striata[J]. , 2017, 29(12): 2077-2083.

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