Dynamic variation rules of protein degradation in flue-cured tobaccos

doi:10.3969/j.issn.1004-1524.2017.12.22

›› 2017, Vol. 29 ›› Issue (12): 2120-2127.DOI: 10.3969/j.issn.1004-1524.2017.12.22

• Food Science • Previous Articles Next Articles

Dynamic variation rules of protein degradation in flue-cured tobaccos

WANG Wanneng¹, XIANG Gangliao¹, ZHAI Yuchen¹, MA Kuoyan², DAI Ya², TAN Lanlan³

1.College of Pharmacy and Biotechnology, Chongqing University of Technology, Chongqing 400054, China;
2. Chongqing China Tobacco Industry Co., Ltd., Chongqing 400060, China;
3. Sichuan China Tobacco Industry Co., Ltd., Chengdu 610000, China

Received:2017-06-06 Online:2017-12-20 Published:2018-01-08

Abstract

Abstract: To analyze and clarify the regularity of protein change in different flue-curing and different flue-curing time of tobacco leaves, and to provide theoretical basis for improving tobacco leaf quality, the dynamic changes and distribution characteristics of tobacco leaves were analyzed by SDS-PAGE and other experimental techniques. The degradation rate of tobacco protein was different at different flue-curing time, and the content of tobacco protein was decreased from 19% to 9% at 0-50 h after flue-curing, and it tended to be gentle in the later flue-curing phase. The molecular weight distribution of leaf protein was between 11.8 and 90.5 ku, and there were significant differences in the content of tobacco protein in the upper, middle and lower leaves. The content of large and small molecular weight protein in the upper leaves was higher than that in the middle and lower leaves, and the content of middle molecular weight in the lower leaves was higher than the others. The degradation rule of different types of protein in different flue-curing stages of tobacco leaf were different.

Key words: tobacco, protein, SDS-PAGE, degradation

CLC Number:

S572

WANG Wanneng, XIANG Gangliao, ZHAI Yuchen, MA Kuoyan, DAI Ya, TAN Lanlan. Dynamic variation rules of protein degradation in flue-cured tobaccos[J]. , 2017, 29(12): 2120-2127.

References

[1] MACKAY J,ERIKSEN M P.The tobacco atlas[M].Geneva: World Health Organization,2002.
[2] VANSUYT G, SOUCHE G, STRACZEK A, et al. Flux of proteins released by wild type and feritin over-experssor tobacco plants: effect of phosphorus and iron nutrition[J]. Plant Physiology and Biochemistry ,2003,41:27-33.
[3] 张柳,王铮,张亚婕,等.烟草叶片衰老期过程中的蛋白质组学分析[J].植物生理学报,2014, 50 (4): 488-500.
ZHANG L, WANG Z, ZHANG Y J, et al. Proteomic analysis of senescing leaf of tobacco[J]. Plant Physiology Journal , 2014, 50 (4): 488-500. (in Chinese with English abstract)
[4] 崔红,冀浩,张华,等.不同生态区烟草叶片蛋白质组学的比较[J]. 生态学报,2008,28(10): 4873-4880.
CUI H, JI H, ZHANG H, et al. Comparative analysis of leaf proteomes between tobacco plants growing in different ecological regions of China[J]. Acta Ecologicalca Sinica ,2008,28(10): 4873-4880. (in Chinese with English abstract)
[5] 姚光明.降低烟叶中蛋白质含量的研究[J].烟草科技,2000 (9):6-8.
YAO G M.Study on reducing the content of protein in tobacco[J]. Tobacco Science & Technology , 2000 (9):6-8.(in Chinese with English abstract)
[6] 王卫峰,陈江华,宋朝鹏,等.密集烤房的研究进展[J].中国烟草科学,2005,26(3):12-14.
WANG W F, CHEN J H, SONG C P, et al. The research progress on bulk curing barn[J]. Chinese Tobacco Science , 2005,26(3):12-14. (in Chinese with English abstract)
[7] 孙敬权,任四海,吴永德.烤烟燃煤密集烤房的改进探讨[J].烟草科技,2004 (9):43-44.
SUN J Q, REN S H, WU Y D. Improvement of bulk curing barn[J]. Tobacco Science & Technology , 2004 (9):43-44. (in Chinese with English abstract)
[8] 李梅云,高家合,王革,等.微生物对烟叶蛋白质含量的影响[J].生物技术通报,2006(增刊1):376-380.
LI M Y, GAO J H, WANG G, et al. Influence of 3 bacteria isolates on protein content of tobacco leaf[J]. Biotechnology Bulletin , 2006(S1):376-380. (in Chinese with English abstract)
[9] 李常军,宫长荣,李锐,等.烘烤过程中烟叶蛋白质与硝态氮代谢规律研究[J].河南农业大学学报,2000,34(1):47-49.
LI C J, GONG C R, LI R, et al. Studies on the metabolism of protein and nitrate nitrogen in tobacco leaf during curing[J]. Journal of Henan Agricultural University , 2000,34(1):47-49. (in Chinese with English abstract)
[10] 李常军,宫长荣,周义和,等.烤烟烘烤过程中变黄温度对氮素代谢的影响[J].中国烟草学报,2001(2):31-35.
LI C J, GONG C R, ZHOU Y H, et al. The effect of temperature in yellowing stage on nitrogen metabolism during tobacco leaf curing process[J]. Acta Tabacaria Sinica ,2001(2):31-35. (in Chinese with English abstract)
[11] 王爱华,徐秀红,王松峰,等.变黄温度对烤烟烘烤过程中生理指标及烤后质量的影响[J].中国烟草学报,2008,14(1):27-31.
WANG A H, XU X H, WANG S F, et al. Effect of temperature in yellowing stage on physiological indices and quality of flue-cured tobacco[J]. Acta Tabacaria Sinica ,2008,14(1):27-31. (in Chinese with English abstract)
[12] 王允白,王宝华,郭承芳,等.影响烤烟评吸质量的主要化学成分研究[J].中国农业科学,1998,31(1):89-91.
WANG Y B, WANG B H, GUO C F, et al. Study on the main chemical components related to smoking quality in flue-curred tobacco[J]. Scientia Aguricultura Sinica ,1998,31(1):89-91. (in Chinese with English abstract)
[13] 赵谋明, 饶国华,林伟锋.烟草蛋白质研究进展[J].烟草科技,2005(4):31-34.
ZHAO M M, RAO G H, LIN W F. Advance in tobacco protein[J]. Tobacco Science & Technology , 2005(4):31-34. (in Chinese with English abstract)
[14] 管方方,卢伟京, 许旭,等. 大豆特征蛋白的SDS-PAGE研究[J].精细化工,2015,32(4):438-442.
GUAN F F, LU W J, XU X, et al. A study on the characteristic soybean protein by SDS-PAGE[J]. Fine Chemicals , 2015,32(4):438-442. (in Chinese with English abstract)
[15] 杨欣,王秋红,王悦,等.不同地区板蓝根蛋白SDS-PAGE分析[J].中国中医药信息杂志,2015,22(7):86-88.
YANG X, WANG Q H, WANG Y, et al. Analysis on isatidis radix protein in different areas by SDS-PAGE[J]. Chinese Journal of Information on Traditional Chinese Medicine ,2015,22(7):86-88. (in Chinese with English abstract)
[16] 吴林坤,王海斌,尤垂淮,等.地黄块根总蛋白质提取及双向电泳条件优化[J].中国中药杂志,2011,36(8) : 984-987.
WU L K, WANG H B, YOU C H, et al. Establishment of extraction method and 2-dimensional electrophoresis conditions for root tuber proteome analysis of Rehmannia glutinosa [J]. China Journal of Chinese Materia Medica , 2011,36(8) : 984-987. (in Chinese with English abstract)
[17] 李常军,宫长荣,肖鹏,等.施氮水平和烘烤条件对烤后烟叶品质和含氮组分的影响[J].中国烟草科学,2001,22(1):4-7.
LI C J, GONG C R, XIAO P, et al. Influence of nitrogen application and flue-curing condition on nitrogen compounds and tobacco quality[J]. Chinese Tobacco Science , 2001,22(1):4-7. (in Chinese with English abstract)
[18] 许东亚, 孙军伟, 杨惠娟,等.酶抑制剂对烤烟成熟期氮代谢及烤后烟叶品质的影响[J]. 烟草科技,2016(3):17-23.
XU D Y, SUN J W, YANG H J, et al. Inhibitory effects of enzyme on nitrogen metabolism at mature stage and quality of cured tobacco leaves[J]. Tobacco Science&Technology ,2016(3):17-23. (in Chinese with English abstract)
[19] 黄天辉,桂金鹏,郑丽沙.利用酶制剂改善再造烟叶品质研究进展[J].东北农业大学学报,2015,46(10):102-108.
HUANG T H, GUI J P, ZHENG L S. Research advances of reconstituted tobacco sheet quality improved with enzyme preparation[J]. Journal of Northeast Agricultural University , 2015,46(10):102-108. (in Chinese with English abstract)
[20] 张亚恒,王芳,张庆明,等. 微生物在烟叶醇化和再造烟叶生产中的应用进展[J]. 湖北农业科学,2015,54(18):4390-4393.
ZHANG Y H, WANG F, ZHANG Q M, et al. Application of microbiology in flue-cured tobacco leaves during aging and reconstituted production[J]. Hubei Agricultural Sciences ,2015,54(18):4390-4393. (in Chinese with English abstract)
[21] ISAACSON T, DAMASCENO C M, SARAVANAN R S, et al. Sample extraction techniques for enhanced proteomic analysis of plant tissues[J]. Nature Protocols ,2010,1(2):769-774.
[22] RODRIGUES A M, KUBITSCHEK-BARREIRA P H, FERNANDES G F, et al. Two-dimensional gel electrophoresis data for proteomic profiling of Sporothrix yeast cells[J]. Data in Brief , 2015,2:32-38.
[23] 宫长荣.烤烟三段式烘烤导论[M].北京:科学出版社,2006.
[24] 刘勇,叶为民, 王玉胜,等.不同烘烤工艺对云烟87烟叶烘烤质量的影响[J]. 中国烟草科学,2016,37(1):56-60.
LIU Y, YE W M, WANG Y S, et al. Effects of different flue-curing processes on tobacco quality of Yunyan 87[J]. Chinese Tobacco Science , 2016,37(1):56-60. (in Chinese with English abstract)
[25] 朱大恒,韩锦峰,张爱萍,等.自然醇化与人工发酵对烤烟化学成分变化的影响比较研究[J].烟草科技,1999 (1):3-5.
ZHU D H, HAN J F, ZHANG A P, et al.Effects of artificial fermentation and aging on the chemical change in flue-cured tobacco[J]. Tobacco Science & Technology , 1999 (1):3-5.(in Chinese with English abstract)
[26] 李晓,刘凤珠,姚光明,等.酶解法改善烟叶吸味品质的试验[J].烟草科技,2002 (3):14-17.
LI X, LIU F Z, YAO G M, et al.Experiments on improvement of smoking quality of tobacco with enzymatic degradation[J]. Tobacco Science & Technology , 2002 (3):14-17. (in Chinese with English abstract)

Dynamic variation rules of protein degradation in flue-cured tobaccos

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