Isolation, identification and antibiotic sensitivity of Morganella morganii from Channa argus

doi:10.3969/j.issn.1004-1524.2018.02.03

›› 2018, Vol. 30 ›› Issue (2): 194-202.DOI: 10.3969/j.issn.1004-1524.2018.02.03

• Animal Science • Previous Articles Next Articles

Isolation, identification and antibiotic sensitivity of Morganella morganii from Channa argus

YANG Yibin^{1, 2}, SONG Yi², YANG Qiuhong^{1, 2}, LIU Yongtao^{1, 2}, YANG Xianle³, AI Xiaohui^{1, 2, *}

1. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
2. Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100141, China;
3. Shanghai Ocean University, Shanghai 201306, China

Received:2017-07-07 Online:2018-02-20 Published:2018-02-11

Abstract

Abstract: Isolation, identification and drug sensitivity test were performed on the pathogen of Channa argus which were suffering from disease in an aquatic farm in Nanchang in order to provide references to effectively prevent and control disease. In this study, a bacterial strain JX15 was isolated from the liver, kidney, spleen, ascites and ulcerated muscle of dying C. argus with the routine separation methods, and identified by morphological observation, physicochemical properties test and molecular biology methods. Moreover, the 16S rRNA and gyrB gene of strain JX15 were amplified and the homology of 16S rRNA and gyrB gene sequences were analyzed, and the phylogenetic tree was constructed. Additionally, an experimental infection test by intraperitoneal injection with isolate was studied, and drug sensitivity test was conducted using K-B paper diffusion method. The results showed that the isolate was identified as Morganella morganii by analyzing biochemical reactions, 16S rRNA and gyrB gene sequences. The fish intraperitoneally-injected with the strain JX15 showed like ones infected by M. morganii naturally. The isolate from artificially-infected fish showed similar morphological and biochemical characteristics to those from naturally infected fish. Therefore, M. morganii was pathogen to C. argus with similar clinical signs as naturally diseased fish. The LD₅₀ of the strain JX15 to C. argus was 3.4×10⁵ cfu·g^-1 according to the Reed-Muench method. Additionally, the results from drug sensitivity test showed that strain JX15 was highly sensitive to 7 medicines, such as florfenicol, norfloxacin, cefotaxime and some else drugs, and it was medium sensitive to furazolidone, chloramphenicol, kanamycin, and streptomycin, but it was resistance to 11 medicines, including ciprofloxacin, oxacillin, penicillin and other durgs. This study proved that the disease in C. argus from an aquatic farm in Nanchang, Jiangxi Province was caused by M. morganii, and this disease could be treated by six medicaments, i.e. florfenicol, cefotaxime, neomycin, tobramycin, gentamicin and amikacin.

Key words: Channa argus, Morganella morganii, isolation and identification, antimicrobial susceptibility

CLC Number:

S941.42

YANG Yibin, SONG Yi, YANG Qiuhong, LIU Yongtao, YANG Xianle, AI Xiaohui. Isolation, identification and antibiotic sensitivity of Morganella morganii from Channa argus[J]. , 2018, 30(2): 194-202.

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Isolation, identification and antibiotic sensitivity of Morganella morganii from Channa argus

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