Abstract: To screen the extraction process of Eupatorium adenophorum and evaluate its acaricidal activity in vivo, 9-oxo-10,11-dehydro ageraphorone (euptox A), which is the main acaricidal active ingredient, was chosen as the detective index, and single factor level test and orthogonal test were carried out to investigate the influence of volume percentage of ethanol, solid to liquid ratio, extraction duration and extraction times on the extraction percentage. The optimal extraction conditions for ethanol extraction were proposed as: 95% volume percentage of ethanol, solid to liquid ratio of 1:20, extraction duration of 1 h, extraction times of three. The skin acute toxicity test and skin irritation test were carried out by sapreading the acaricidal preparations of E. adenophorum on the integral and damaged back skin of healthy rabbits. The results showed that after using the drug for 72 h, rabbits did not show any reaction of acute toxicity and irritation. In acaricidal clinical test, 30 rabbits with psoroptes mites and 26 rabbits with scabies mites were respectively divided into six groups. The animals in group A and B were treated with the acaricidal preparation contained azone and raw drug (E. adenophorum) 1.0, 0.5 g·mL^-1, respectively. The animals in groups C and D were treated with the acaricidal preparation contained raw drug 1.0, 0.5 g·mL^-1, which did not contain azone, respectively. The animals in group E and F were injected with ivermectin 0.2 mg (positive controls) and embrocated 0.9% normal saline on skin (negative controls), respectively. The results showed that,whether for the psoroptes mite group or the scabies mite group, the prepatation with high dose of E. adenophorum and azone worked best.

Key words: Eupatorium adenophorum, extraction process, clincal acariasis, acaricidal preparation, skin safety test