Isolation and susceptibility test of pathogens responsible for porcine postweaning diarrhea

doi:10.3969/j.issn.1004-1524.2018.04.07

›› 2018, Vol. 30 ›› Issue (4): 568-575.DOI: 10.3969/j.issn.1004-1524.2018.04.07

• Animal Science • Previous Articles Next Articles

Isolation and susceptibility test of pathogens responsible for porcine postweaning diarrhea

WANG Jing¹, DIAO Xiaolong², YANG Maoyi¹, WANG Quanfang¹, CHEN Xiaolan¹, ZHANG Long^{1, *}

1. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
2. Zhongchong Sino Biotech Taizhou Co., Ltd, Taizhou 225300, China

Received:2017-07-31 Online:2018-04-20 Published:2018-04-19

Abstract

Abstract: In order to investigate the main causes of diarrhea of piglets in Taizhou, Jiangsu Province, 70 rectal swab samples were collected from the live diarrheic piglets, and 136 strains of bacteria were isolated by MA plates, in which 119 strains of Escherichia coli, 5 strains of Salmonella, 4 strains of Shigella, 8 strains of Cronobacter sakazakii were characterized by biochemical identification and 16S rDNA gene sequence analysis. 81 strains of pathogenic E. coli were confirmed according to the animal test. Among them, 36 strains of pathogenic E. coli were identified belonging to 6 serotypes by agglutination tests, and the predominant serotypes were O₇₈ and O₁₀₁. In addition, among 81 pathogenic E. coli, 20 isolates were ST1+ST2, 44 isolates were LT1+ST2,while others were ST2. The drug resistance of pathogenic isolates were tested by Kirby-Barer method, and the results showed that more than 75% pathogenicity isolates were sensitive to ceftazidime, amikacin and ofloxacin, but resistant to tetracycline, cefuroxime, kanamycin and doxycycline seriously,and there were serious multi-drug resistance. These results would provide scientific data for the prevention and control of porcine postweaning diarrhea disease in Taizhou area.

Key words: O-antigen, susceptibility test, drug resistance, enterotoxin gene

CLC Number:

S856

WANG Jing, DIAO Xiaolong, YANG Maoyi, WANG Quanfang, CHEN Xiaolan, ZHANG Long. Isolation and susceptibility test of pathogens responsible for porcine postweaning diarrhea[J]. , 2018, 30(4): 568-575.

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Isolation and susceptibility test of pathogens responsible for porcine postweaning diarrhea

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