Immune effect of soybean antigen protein in piglets

doi:10.3969/j.issn.1004-1524.2018.04.06

›› 2018, Vol. 30 ›› Issue (4): 560-567.DOI: 10.3969/j.issn.1004-1524.2018.04.06

• Animal Science • Previous Articles Next Articles

Immune effect of soybean antigen protein in piglets

ZHANG Yu, WANG Yuanhong, TANG Xuebing, PENG Chenglu, SHU Yingshuang, CAO Chengming, FENG Shibin, LI Yu, WANG Xichun, WU Jinjie^*

College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received:2017-08-14 Online:2018-04-20 Published:2018-04-19

Abstract

Abstract: Piglets were immunized by oral administrating or injecting soybean antigen protein immunizing agents to study the effects of soybean antigen protein immunizing agents on serum antibody titers of β-conglycinin (7S), glycinin (11S), and serum IgA, IgM, IgE, IgG and IL-4 levels in piglets. Seventy “Duroc×Landrace×Yorkshire” crossbred piglets (7-day-old) were randomly allocated into group A (control group), group B (7S sensitized group), group C (11S sensitized group), group D (7S orally immunized +sensitized group), group E (11S orally immunized +sensitized group), group F (7S injecting immunized +sensitized group) and group G (11S injecting immunized + sensitized group) with ten piglets each. Piglets were orally taken or injected with soybean antigen protein immunizing agents according to design at 7 days old and were immunized again at 14 days old, and the piglets in group A were injected with physiological saline. All the piglets were weaned at 21 days old. From 21 days old, piglets in group A were fed with basal diets, and piglets in group B, D, F and group C, E, G were fed with basal diets supplemented with 5% 7S or 5% 11S, respectively for 7 d. The skin sensitization test was conducted in piglets at 27 days old. Blood samples of ten piglets from each group were taken from vena cava anterior at 7, 14, 21 and 27 days old, and the sera was separated to measure IgA, IgM, IgE, IgG and IL-4 levels by ELISA method and antibody titers of 7S and 11S by agar diffusion method. The results showed that the skin sensitization valuing results of piglets were group B and C>group D and E>group A, F and G, meanwhile the sensitization effect of 7S was higher than that of 11S in piglets. Serum antibody titers in group F and G were higher than those in group D and E at the age of 21 days, and serum antibody titers in group B and C were higher than those in group D, E, F and G at the age of 27 days. Serum IgG, IL-4 levels of piglets in group F were significantly (P<0.05) higher than those in group A at 14 days old. Compared with group A, B and C, serum IgM, IgE, IgG and IL-4 levels of piglets were significantly (P<0.05) increased in group D, E, F and G at 21 days old, meanwhile those in group F and G were significantly (P<0.05) higher than those in group D and E, except IgG level in group G. At 27 days old, serum IgE, IgG and IL-4 levels of piglets in treatment groups were significantly (P<0.01) higher than those in group A, and those in group B were significantly (P<0.05) higher than those in group C, meanwhile those in group D and E were significantly (P<0.05) higher than those in group F and G. The experimental results suggested that the sensitization effect of 7S was higher than that of 11S in piglets. Soybean antigen protein immunizing agents could produce immune-protective effect in piglets, and the immune effect of 7S was prior to that of 11S. In addition, the effect of injecting immunization was prior to oral immunization with the same dose.

Key words: glycinin (11S), β-conglycinin(7S);, IgA, IgM, IgE, IgG, IL-4

CLC Number:

ZHANG Yu, WANG Yuanhong, TANG Xuebing, PENG Chenglu, SHU Yingshuang, CAO Chengming, FENG Shibin, LI Yu, WANG Xichun, WU Jinjie. Immune effect of soybean antigen protein in piglets[J]. , 2018, 30(4): 560-567.

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Immune effect of soybean antigen protein in piglets

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