Expression and subcellular localization of bone marrow stromal antigen 2 gene of Capra hircus

doi:10.3969/j.issn.1004-1524.2018.05.04

›› 2018, Vol. 30 ›› Issue (5): 702-706.DOI: 10.3969/j.issn.1004-1524.2018.05.04

• Animal Science • Previous Articles Next Articles

Expression and subcellular localization of bone marrow stromal antigen 2 gene of Capra hircus

ZHANG Li^1,2, LIU Teng², MIAO Qiuhong², TANG Jingyu², ZHU Jie², DONG Dandan², CHEN Zongyan², WANG Guijun^1,*, LIU Guangqing^2,*

1.College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2.Shanghai Veterinary Research Institute, CAAS, Shanghai 200241, China

Received:2017-07-12 Online:2018-05-20 Published:2018-05-23

Abstract

Abstract: BST-2 gene (Capra hircus bone marrow stromal antigen 2 gene) was amplified and cloned into pGEX-4T-1 and p3×Flag-CMV-14, respectively. The recombinant plasmids were named as pGEX-BST-2 and p3×Flag-BST-2, respectively. Firstly, pGEX-BST-2 was transformed into E.coli BL21(DE3), then the protein expression was induced by IPTG. The results of SDS-PAGE and Western blot analysis showed that the molecular weight of the recombinant protein was about 42 ku. Finally, Vero cells were transfected with p3×Flag-BST-2 through Lipofectamine 2 000 reagent, and the cells were analyzed by confocal microscopy.

Key words: BST-2 gene (bone marrow stromal antigen 2 gene), prokaryotic expression, eukaryotic expression, subcellular localization, Capra hiracs

CLC Number:

S827

ZHANG Li, LIU Teng, MIAO Qiuhong, TANG Jingyu, ZHU Jie, DONG Dandan, CHEN Zongyan, WANG Guijun, LIU Guangqing. Expression and subcellular localization of bone marrow stromal antigen 2 gene of Capra hircus[J]. , 2018, 30(5): 702-706.

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Expression and subcellular localization of bone marrow stromal antigen 2 gene of Capra hircus

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