Evolutionary and molecular characteristics of M genes of porcine epidemic diarrhea viruses in western Fujian, China

doi:10.3969/j.issn.1004-1524.2018.06.08

›› 2018, Vol. 30 ›› Issue (6): 939-945.DOI: 10.3969/j.issn.1004-1524.2018.06.08

• Animal Science • Previous Articles Next Articles

Evolutionary and molecular characteristics of M genes of porcine epidemic diarrhea viruses in western Fujian, China

DONG Bo^{1, 2}, AN Yongshuai¹, DAI Ailing^{1, 2}, LI Xiaohua^{1, 2}, YANG Xiaoyan^{1, 2, *}

1. College of Life Science of Longyan University, Longyan 364012, China;
2. Fujian Engineering Research Center for Swine Disease Control and Prevention, Longyan 364012, China

Received:2017-09-28 Online:2018-06-20 Published:2018-07-02

Abstract

Abstract: To elucidate evolutionary characteristics of the memberane (M) genes of porcine epidemic diarrhea viruses in Fujian, China, 12 samples collected from Fujian province during 2015 to 2017 were screened by multiplex RT-PCR,M genes of PEDV viruses from positive samples were amplified by RT-PCR and cloned into pMD18-T vectors for sequencing respectively. The sequence data were done alignment and phylogenetic analysis with sequences from known reference strains. The M genes from 12 representative positive samples shared 100.0% identity at amino acid level. 12 representative positive samples are remote to classical strain CV777, SM98 and virulent DR13. 12 representative strains belong to the genotype variant strain. Molecular structure characteristics analysis showed that M gene is highly conservative and could be an optimal candidate protein for vaccine development.

Key words: porcine epidemic diarrhea virus, M gene, evolutionary characteristics, molecular characteristic

CLC Number:

S858.28

DONG Bo, AN Yongshuai, DAI Ailing, LI Xiaohua, YANG Xiaoyan. Evolutionary and molecular characteristics of M genes of porcine epidemic diarrhea viruses in western Fujian, China[J]. , 2018, 30(6): 939-945.

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Evolutionary and molecular characteristics of M genes of porcine epidemic diarrhea viruses in western Fujian, China

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