Selection of reference genes in wheat infected by Chinese wheat mosaic virus (CWMV)

doi:10.3969/j.issn.1004-1524.2018.07.11

›› 2018, Vol. 30 ›› Issue (7): 1182-1187.DOI: 10.3969/j.issn.1004-1524.2018.07.11

• Plant Protection • Previous Articles Next Articles

Selection of reference genes in wheat infected by Chinese wheat mosaic virus (CWMV)

WU Ne^{1, 2}, CHEN Xuan^{2, 3}, JIANG Yaoyao^{2, 3}, ZHANG Tianye^{2, 3}, YANG Jian², ZHU Tongquan⁴, ZHANG Hengmu^{2, *}, CHEN Jianping^{1, 2, *}

1. Collge of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300, China;
2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China;
4. Zhumadian Academy of Agriculture Sciences, Zhumadian 463000, China

Received:2017-09-13 Online:2018-07-20 Published:2018-08-02

Abstract

Abstract: Quantitative PCR (qRT-PCR) has been widely applied to the expression analysis of functional genes. The selection of appropriate reference genes is required for the statistical analysis of qRT-PCR data. To select the suitable reference genes in wheat infected by Chinese wheat mosaic virus(CWMV), 10 housekeeping genes were screened by calculations of PCR efficiencies and melting curve analysis, suggesting that 8 out of 10 genes could be used as candidate reference genes. And then their temporal and spatial expression patterns were investigated by qRT-PCR in the different tissues and at the different stages of wheat inoculated with CWMV. These quantitative data were further evaluated by the computer programs geNorm and NormFinder in this study. The analysis results consistently revealed that two genes, 26S and CDC, were stably expressed in all tested tissues and CWMV-infected wheat samples, indicating that the combination of 26S and CDC could be selected as reference genes for expression analysis of functional gene during the interaction between CWMV and wheat.

Key words: reference gene, wheat, Chinese wheat mosaic virus, qRT-PCR

CLC Number:

S435.121

WU Ne, CHEN Xuan, JIANG Yaoyao, ZHANG Tianye, YANG Jian, ZHU Tongquan, ZHANG Hengmu, CHEN Jianping. Selection of reference genes in wheat infected by Chinese wheat mosaic virus (CWMV)[J]. , 2018, 30(7): 1182-1187.

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Selection of reference genes in wheat infected by Chinese wheat mosaic virus (CWMV)

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