Cloning and expression analysis of oxalate oxidase gene MsOXO from Medicago sativa

doi:10.3969/j.issn.1004-1524.2019.01.02

›› 2019, Vol. 31 ›› Issue (1): 11-19.DOI: 10.3969/j.issn.1004-1524.2019.01.02

• Crop Science • Previous Articles Next Articles

Cloning and expression analysis of oxalate oxidase gene MsOXO from Medicago sativa

XU Qingsong^1,2, ZHAO Jia^1,2, WEI Yunmin^1,2, HAN Rongrong^1,2, LIU Lusheng^1,2, JIANG Caode^1,2, YU Yong-xiong^1,2,*

1. College of Animal Science and Technology, Southwest University, Chongqing 400715, China;
2. College Herbivore Engineering Center of Chongqing, Chongqing 400715, China

Received:2018-08-03 Online:2019-01-25 Published:2019-04-09

Abstract

Abstract: Oxalate oxidase plays an important role in defence of various pathogens such as Sclerotinia and can be used for plant improvement. To explore the role of oxalate oxidase in the defense of Sclerotinia sclerotiorum, alfalfa was used for material in this study, an oxalate oxidase gene, MsOXO, was successfully cloned. Subcellular localization of MsOXO was performed using the transient expression of tobacco, and the result showed that MsOXO was located in cell wall. The results of qRT-PCR showed that MsOXO was expressed in roots, stems and leaves of alfalfa, but was lower in roots and stems, which might be the reason why the root neck and stem base were more susceptible to infection by the pathogen. The up-regulated expression of MsOXO gene was induced by exogenous oxalic acid 50 μmol·L^-1 treated root or 100 μmol·L^-1 treated stem and exogenous salicylic acid 100 μmol·L^-1 treated root.

Key words: Medicago sativa, oxalate oxidase, gene cloning, subcellular localization

CLC Number:

S963.22⁺3.3

XU Qingsong, ZHAO Jia, WEI Yunmin, HAN Rongrong, LIU Lusheng, JIANG Caode, YU Yong-xiong. Cloning and expression analysis of oxalate oxidase gene MsOXO from Medicago sativa[J]. , 2019, 31(1): 11-19.

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Cloning and expression analysis of oxalate oxidase gene MsOXO from Medicago sativa

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