CaMV35S promoter and its application and detection in transgenic crops

doi:10.3969/j.issn.1004-1524.2019.01.21

›› 2019, Vol. 31 ›› Issue (1): 161-170.DOI: 10.3969/j.issn.1004-1524.2019.01.21

• Review • Previous Articles

CaMV35S promoter and its application and detection in transgenic crops

TANG Ting¹, XIE Shilong¹, ZHU Xuan¹, XU Junfeng², TANG Jun¹, WANG Xiaofu^2,*

1. College of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China;
2. Institute of Agricultural Product Quality Standards, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-09-12 Online:2019-01-25 Published:2019-04-09

Abstract

Abstract: CaMV35S promoter is one of the most important promoter that widely used in plant genetic engineering. It plays a key role in the safety evaluation and detection of transgenic plants. Understanding the origin and development of CaMV35S promoter is the prerequisite for carrying out safety assessment and detection of transgenic plants. In this review, history of CaMV35S promoter and its structure and function were briefly described. Then, application of CaMV35S promoter in transgenic crops and the corresponding detection methods were analyzed and summarized. Finally, aiming at the problems existing in detection of CaMV35S promoter in transgenic crops, it was proposed that in future studies, sequence information and detection data of related transgenic crops should be communicated and shared, meanwhile, data sharing and joint verification should be carried out among various testing institutions or laboratories, so as to establish a relatively uniform standard detection method for CaMV35S promoter.

Key words: CaMV35S promoter, transgenic crop, enhancer, PCR

CLC Number:

Q943.2

TANG Ting, XIE Shilong, ZHU Xuan, XU Junfeng, TANG Jun, WANG Xiaofu. CaMV35S promoter and its application and detection in transgenic crops[J]. , 2019, 31(1): 161-170.

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CaMV35S promoter and its application and detection in transgenic crops

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