Distribution and resistance evaluation of 5 rice blast-resistant genes in cultivated rice varieties in Zhejiang

doi:10.3969/j.issn.1004-1524.2019.06.09

›› 2019, Vol. 31 ›› Issue (6): 922-929.DOI: 10.3969/j.issn.1004-1524.2019.06.09

• Plant Protection • Previous Articles Next Articles

Distribution and resistance evaluation of 5 rice blast-resistant genes in cultivated rice varieties in Zhejiang

HE Haiyan^{1, 2, 3}, CHAI Rongyao^{2, 3}, QIU Haiping^{2, 3}, MAO Xueqin^{2, 3}, WANG Yanli^{2, 3}, SUN Guocang^{2, 3, *}

1. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100080,China;
2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China;
3. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Hangzhou 310021,China

Received:2018-11-13 Online:2019-06-25 Published:2019-06-26

Abstract

Abstract: In order to clarify the distribution of blast resistance genes in some rice cultivars and blast-resistant cultivars in Zhejiang Province, five functional molecular markers based on blast resistance allele sequences were used to detect Pit, Pib, Pii/Pi3/Pi5, Pia and Pi1 resistance genes in 40 main rice varieties in Zhejiang Province and 6 Chinese rice blast identification varieties. The results showed that the distribution frequencies of the five resistance genes were different, Pib gene was most widely distributed, accounting for 63.04%, followed by Pia, accounting for 58.70%, Pii/Pi3/Pi5 and Pit resistance genes were less, accounting for 21.74% and 10.87% respectively. At the same time, the resistance level of 46 rice varieties to Magnaporthe grisea was detected by 141 strains collected in the field from 2015 to 2017. The results showed that the resistance frequency of 15 varieties was more than 70%. Most of the varieties carried one or two resistance genes. The resistance level of varieties with multiple resistance genes was relatively higher.

Key words: rice, rice leaf blast, resistance gene, molecular marker

CLC Number:

S435.111.4⁺1

HE Haiyan, CHAI Rongyao, QIU Haiping, MAO Xueqin, WANG Yanli, SUN Guocang. Distribution and resistance evaluation of 5 rice blast-resistant genes in cultivated rice varieties in Zhejiang[J]. , 2019, 31(6): 922-929.

References

[1] 陆明红, 刘万才, 朱凤, 等. 2014年稻瘟病重发原因分析与治理对策探讨[J]. 中国植保导刊, 2015, 35(6):35-39.
LU M H,LIU W C,ZHU F, et al.Cause analysis and countermeasures of serious rice blast in 2014[J]. China Plant Protection, 2015, 35(6):35-39. (in Chinese)
[2] 谢子正,许渭根,李仁忠,等. 2014年浙江省水稻稻瘟病流行特点及原因分析[J]. 中国植保导刊, 2015, 35(3):58-60.
XIE Z Z, XU W G, LI R Z, et al.Epidemic characteristics and causes of rice blast in Zhejiang Province in 2014[J]. China Plant Protection, 2015, 35(3): 58-60. (in Chinese)
[3] WANG Z X, YANO M, YAMANOUCHI U, et al.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes[J]. The Plant Journal,1999,19(1): 55-64.
[4] 华丽霞, 汪文娟, 陈深, 等. 抗稻瘟病Pi2/9/z-t基因特异性分子标记的开发[J]. 中国水稻科学, 2015, 29(3): 305-310.
HUA L X, WANG W J, CHEN S, et al.Development of specific DNA markers for detecting the rice blast resistance gene alleles Pi2/9/z-t[J]. Chinese Journal of Rice Science, 2015, 29(3): 305-310.(in Chinese with English abstract)
[5] 孙国昌, 杜新法, 柴荣耀, 等. 水稻主要抗瘟基因对我国菌株的抗性分析和利用评价[J]. 植物保护学报, 1999, 26(2): 103-106.
SUN G C, DU X F, CHAI R Y, et al.Analysis and utilization of major gene resistance to blast in China[J]. Journal of Plant Protection, 1999, 26(2): 103-106.(in Chinese with English abstract)
[6] International Rice Research Institute. Standard evolution system for rice[M]. 4th ed. Manila: IRRI, 1996: 17-18.
[7] HAYASHI K, YOSHIDA H, ASHIKAWA I.Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes[J]. Theoretical and Applied Genetics, 2006, 113(2): 251-260.
[8] FJELLSTROM R, CONAWAY-BORMANS C A,MCCLUNG A M, et al.Development of DNA markers suitable for marker assisted selection of three genes conferring resistance to multiple pathotypes[J]. Crop Science, 2004, 44(5): 1790.
[9] 刘洋, 徐培洲, 张红宇, 等. 水稻抗稻瘟病Pib基因的分子标记辅助选择与应用[J]. 中国农业科学, 2008, 41(1): 9-14.
LIU Y, XU P Z, ZHANG H Y, et al.Marker-assisted selection and application of blast resistant gene Pib in rice[J]. Scientia Agricultura Sinica, 2008, 41(1): 9-14.(in Chinese with English abstract)
[10] YI G, LEE S K, HONG Y K, et al.Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea[J]. Theoretical and Applied Genetics, 2004, 109(5): 978-985.
[11] OKUYAMA Y, KANZAKI H, ABE A, et al.A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes[J]. The Plant Journal, 2011, 66(3): 467-479.
[12] 刘开强, 伍豪, 颜群, 等. 水稻抗稻瘟病基因Pi1的特异性分子标记开发及利用[J]. 西南农业学报, 2016, 29(6): 1241-1244.
LIU K Q, WU H, YAN Q, et al.Development and application of specific marker of blast resistance gene Pi1 in rice[J]. Southwest China Journal of Agricultural Sciences, 2016, 29(6): 1241-1244.(in Chinese with English abstract)
[13] 凌忠专. 稻瘟病研究论文集[M]. 北京: 中国农业出版社,2005: 231-242.
[14] 张善磊,孙旭超,陈涛,等.Pi-ta、Pi-5、Pi-km和Pi-b基因在粳稻品种(系)中的分布及对穗颈瘟的抗性[J].江苏农业学报,2018,34(5):961-971.
ZHANG S L,SUN X C,CHEN T, et al.Distribution of Pi-ta,Pi-5,Pi-km and Pi-b genes in japonica rice varieties (lines) and their relationship with neck blast resistance[J].Jiangsu Journal of Agricultural Sciences, 2018,34(5):961-971.(in Chinese with English abstract)
[15] 柴荣耀, 杜新法, 毛雪琴, 等. 水稻不同类型品种上的稻瘟病菌群体小种的演变[J]. 浙江农业学报, 1999, 11(6): 297-300.
CHAI R Y, DU X F, MAO X Q, et al.Race evolution of Magnaporthe grisea on different rice varieties[J]. Acta Agriculturae Zhejiangensis, 1999, 11(6): 297-300.(in Chinese with English abstract)
[16] JONES J D G, DANGL J L. The plant immune system[J]. Nature, 2006, 444(7117): 323-329.
[17] 何峰, 张浩, 刘金灵, 等. 水稻抗稻瘟病天然免疫机制及抗病育种新策略[J]. 遗传, 2014, 36(8): 756-765.
HE F, ZHANG H, LIU J L, et al.Recent advances in understanding the innate immune mechanisms and developing new disease resistance breeding strategies against the rice blast fungus Magnaporthe oryzae in rice[J]. Hereditas, 2014, 36(8): 756-765.(in Chinese with English abstract)
[18] CHEN J, SHI Y F, LIU W Z, et al.A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae[J]. Journal of Genetics and Genomics, 2011, 38(5): 209-216.
[19] FUKUOKA S, YAMAMOTO S I, MIZOBUCHI R, et al.Multiple functional polymorphisms in a single disease resistance gene in rice enhance durable resistance to blast[J]. Scientific Reports, 2015, 4: 4550.
[20] LIU X Q, LIN F, WANG L, et al.The in silico map-based cloning of Pi36, a rice coiled-coil-nucleotide-binding site-leucine-rich repeat gene that confers race-specific resistance to the blast fungus[J]. Genetics, 2007, 176(4): 2541-2549.
[21] LIN F, CHEN S, QUE Z Q, et al.The blast resistance gene Pi37 encodes a nucleotide binding site-leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1[J]. Genetics, 2007, 177(3): 1871-1880.
[22] KANG S.ThePWLHost specificity gene family in the blast fungus Magnaporthe grisea[J]. Molecular Plant-Microbe Interactions, 1995, 8(6): 939.
[23] SWEIGARD J A.Identification, cloning, and characterization of PWL2, a gene for host species specificity in the rice blast fungus[J]. The Plant Cell, 1995, 7(8): 1221-1233.
[24] ORBACH M J, FARRALL L, SWEIGARD J A, et al.A telomeric avirulence gene determines efficacy for the rice blast resistance gene Pi-ta[J]. The Plant Cell, 2000, 12(11): 2019.
[25] LI W, WANG B H, WU J, et al.The Magnaporthe oryzaea virulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-T[J]. Molecular Plant-Microbe Interactions, 2009, 22(4): 411-420.
[26] ZHAO K Y, TUNG C W, EIZENGA G C, et al.Genome-wide association mapping reveals a rich genetic architecture of complex traits in Oryza sativa[J]. Nature Communications, 2011, 2: 467.
[27] KANG H, WANG Y, PENG S, et al.Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae[J]. Molecular Plant Pathology, 2015, 17(6):959-972.

Distribution and resistance evaluation of 5 rice blast-resistant genes in cultivated rice varieties in Zhejiang

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