›› 2019, Vol. 31 ›› Issue (12): 2005-2010.DOI: 10.3969/j.issn.1004-1524.2019.12.08

• Animal Science • Previous Articles     Next Articles

Research on immunological effect of chitosan oligosaccharide on subunit vaccine of giant panda rotavirus VP6-VP7 protein

WEN Jifeng, SHEN Huanhuan, YIN Wenqi, GONG Yongping, YI Keke, DENG Ying, YAN Qigui*   

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chendu 611130, China
  • Received:2019-03-01 Online:2019-12-25 Published:2019-12-25

Abstract: To research the immunopotentiation effect of chitosan oligosaccharide on the giant panda rotavirus (GPRV) recombinant protein VP6-VP7, prokaryotic expressed recombinant VP6-VP7 protein were used as antigen and added with 2.0 mg·mL-1 chitosan oligosaccharide as an immunoadjuvant to make a GPRV VP6-VP7-COS subunit vaccine.78 SPF Kunming mice were randomly divided into 4 groups. Group PC immunopurified recombinant VP6-VP7 protein, group A immunized VP6-VP7-COS subunit vaccine, group B immunized VP6-VP7 aluminum hydroxide gel adjuvant subunit vaccine, group NC injected with PBS. At 0, 15 and 30 days, immunization was carried out. After each vaccination, 6 mice were randomly selected from each group to collect serum, and serum IgG, IgA antibody titer, T lymphocyte transformation rate and cytokine content were measured. Muscle tissues of the injection site of each group were subjected to histopathological analysis to evaluate the toxic side effects of COS as an immunoadjuvant. The results showed that the IgG level of VP6-VP7-aluminum hydroxide gel adjuvant group and VP6-VP7-COS group were significantly (P<0.05) higher than the other groups. The IgA level in the VP6-VP7-COS group was significantly (P<0.05) higher than that in the other groups. The ability of VP6-VP7-aluminum hydroxide gel adjuvant group and VP6-VP7-COS physical mixing group to induce T lymphocyte proliferation were significantly (P<0.05)higher than that of VP6-VP7 protein group. IL-2, IL-4, IL-5 and IL-γ in VP6-VP7-COS group and VP6-VP7-aluminum hydroxide gel adjuvant group were significantly (P<0.05) higher than the other groups. Histopathological analysis showed that there were no pathological changes in the muscle tissue of the injected site of VP6-VP7 subunit vaccine immunized with COS as adjuvant. This study showed that GPRV recombinant protein VP6-VP7 can significantly induce the immune response in animals, and chitosan oligosaccharide had a better immune enhancement effect on GPRV VP6-VP7 protein. This study provided a reference for the development of safe, non-side-effect and high immunoprotective GPRV subunit vaccines.

Key words: giant panda rotavirusv, VP6-VP7 protein, chitosan oligosaccharide, immune enhancement, subunit vaccine

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