Effect of variation of PRKCB gene intron 14 in Sansui ducks on eggshell quality

doi:10.3969/j.issn.1004-1524.2020.09.06

›› 2020, Vol. 32 ›› Issue (9): 1574-1580.DOI: 10.3969/j.issn.1004-1524.2020.09.06

• Animal Science • Previous Articles Next Articles

Effect of variation of PRKCB gene intron 14 in Sansui ducks on eggshell quality

LIAO Chaomei, YOU Minfang, TAN Guanghui, LI Jiezhang, ZHANG Yiyu^*, QIN Yuanyu, LIU Ruoyu

Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2020-01-08 Online:2020-09-25 Published:2020-10-10

Abstract

Abstract: To explore the genetic effect of PRKCB gene on eggshell quality of Sansui duck, direct sequencing of PCR products, MegaAlign program of DNAStar software and Chromas software were used to screen and identify the genetic locus variation of (Anas platyrhyncha domestica) PRKCB gene. The correlation between SNP loci and eggshell traits were analysed by SPSS 18.0 software. The results indicated that three moderately polymorphic novel SNPs of g.1158465 C>T, g.1158519 G>A and g.1158524 G>A were detected in intron 14 of PRKCB gene in Sansui duck. Three genotypes were produced in each SNP. The genotype distribution of g.1158519 G>A and g.1158524 G>A were in accordance with Hardy-Weinberg equilibrium, while that of g.1158465 C>T was significantly (P<0.05) deviated from Hardy-Weinberg equilibrium. There was a strong linkage disequilibrium between g.1158519 G>A and g.1158465 C>T loci. The combination of three SNPs composed of 5 haplotypes and 8 diplotypes. The frequency of haplotype H5 (TGG) and diplotype H2H5 was 0.492 and 0.200, respectively. Association analysis showed that the eggshell thickness of individuals with AG genotype at g.1158519 G>A locus was significantly (P<0.05) higher than that of individuals with GG genotype, and the eggshell thickness of diplotype H1H3 individuals was significantly (P<0.05) higher than that of H3H5 individuals. The results suggested that the genetic variation of the intron 14 in PRKCB gene can affect duck eggshell quality, and g.1158519 G>A locus may be used as a genetic marker to improve duck eggshell quality.

Key words: PRKCB gene, Sansui ducks, SNP identification, eggshell quality, association analysis

CLC Number:

S834

LIAO Chaomei, YOU Minfang, TAN Guanghui, LI Jiezhang, ZHANG Yiyu, QIN Yuanyu, LIU Ruoyu. Effect of variation of PRKCB gene intron 14 in Sansui ducks on eggshell quality[J]. , 2020, 32(9): 1574-1580.

References

[1] 张家才, 熊铭鑫, 张聪, 等. 孕酮对鸡蛋壳品质的影响[J]. 中国畜牧杂志, 2020, 56(1): 128-131.
ZHANG J C, XIONG M X, ZHANG C, et al.Effect of progesterone on eggshell quality[J]. Chinese Journal of Animal Science, 2020, 56(1): 128-131.(in Chinese with English abstract)
[2] ZHANG Q, ZHU F, LIU L, et al.Integrating transcriptome and genome re-sequencing data to identify key genes and mutations affecting chicken eggshell qualities[J]. PLoS One, 2015, 10(5): e0125890.
[3] LUNDHOLM C E, BARTONEK M.Furosemide decreases eggshell thickness and inhibits 45 Ca²⁺ uptake by a subcellular fraction of eggshell gland mucosa of the domestic fowl[J]. Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 1992, 101(2): 317-320.
[4] SUBEDI K P, SON M J, CHIDIPI B, et al.Signaling pathway for endothelin-1-and phenylephrine-induced cAMP response element binding protein activation in rat ventricular myocytes: role of inositol 1, 4, 5-trisphosphate receptors and CaMKII[J]. Cellular Physiology and Biochemistry, 2017, 41(1): 399-412.
[5] LARTEY J, SMITH M, PAWADE J, et al.Up-regulation of myometrial RHO effector proteins (PKN₁ and DIAPH₁) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor[J]. Biology of Reproduction, 2007, 76(6): 971-982.
[6] 利凯, 马利芹, 董世山, 等. 肉鸡腹水综合征患鸡右心组织钙沉积和Ca²⁺-ATPase活性变化[J]. 畜牧兽医学报, 2007, 38(6): 595-600.
LI K, MA L Q, DONG S S, et al.The change of Ca²⁺ and Ca²⁺-ATPase of right ventricular myocardium in ascites syndrome of broiler chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2007, 38(6): 595-600.(in Chinese with English abstract)
[7] GERSCHÜTZ A, HEINSEN H, GRÜNBLATT E, et al. Neuron-specific alterations in signal transduction pathways associated with Alzheimer's disease[J]. Journal of Alzheimer's Disease, 2014, 40(1): 135-142.
[8] ZHU Z W, YANG L L, ZHANG Y H, et al.Increased expression of PRKCB mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus[J]. Annals of Human Genetics, 2018, 82(4): 200-205.
[9] LARTEY J, SMITH M, PAWADE J, et al.Up-regulation of myometrial RHO effector proteins (PKN₁ and DIAPH₁) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor[J]. Biology of Reproduction, 2007, 76(6): 971-982.
[10] TREXLER A J, TARASKA J W.Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells[J]. Cell Calcium, 2017, 67: 1-10.
[11] 杨宁. 家禽生产学[M]. 2版. 北京: 中国农业出版社, 2010.
[12] 白俊艳, 卢军浩, 付学言, 等. 蛋用鹌鹑IGF-1 R基因的多态性与体尺性状相关性分析[J]. 浙江农业学报, 2020, 32(3): 398-405.
BAI J Y, LU J H, FU X Y, et al.Analysis of correlation between polymorphism of IGF-1R gene and body size traits in eggquail[J]. Acta Agriculturae Zhejiangensis, 2020, 32(3): 398-405.(in Chinese with English abstract)
[13] PATERGNANI S, MARCHI S, RIMESSI A, et al.PRKCB/protein kinase C, beta and the mitochondrial axis as key regulators of autophagy[J]. Autophagy, 2013, 9(9): 1367-1385.
[14] LACHANCE K, BARHDADI A, MONGRAIN I, et al.PRKCB is associated with calcineurin inhibitor-induced renal dysfunction in heart transplant recipients[J]. Pharmacogenetics and Genomics, 2012, 22(5): 336-343.
[15] SERROTE C M L, REINIGER L R S, SILVA K B, et al. Determining the polymorphism information content of a molecular marker[J]. Gene, 2020, 726: 144175.
[16] MAYO O.A century of Hardy-Weinberg equilibrium[J]. Twin Research and Human Genetics, 2008, 11(3): 249-256.
[17] 赵宪林, 曹少杰, 王俊全. MC4R基因多态性及基因型组合与藏羊生长性状的关联分析[J]. 农业生物技术学报, 2018, 26(3): 429-436.
ZHAO X L, CAO S J, WANG J Q.Association analysis of MC4R gene polymorphisms and genotype combination with growth traits of Tibetan sheep(Ovis aries)[J]. Journal of Agricultural Biotechnology, 2018, 26(3): 429-436.(in Chinese with English abstract)
[18] SUPEK F, Miñana B, VALCÁRCEL J, et al. Synonymous mutations frequently act as driver mutations in human cancers[J]. Cell, 2014, 156(6): 1324-1335.
[19] AKEY J, JIN L, XIONG M.Haplotypes vs single marker linkage disequilibrium tests: what do we gain?[J]. European Journal of Human Genetics, 2001, 9(4): 291-300.

Effect of variation of PRKCB gene intron 14 in Sansui ducks on eggshell quality

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

Comments

[1]	HU Qianwen, XU Yanhao, WANG Rong, ZHANG Wenying, HUA Wei, LYU Chao. Association analysis of four spike traits in barley [J]. , 2020, 32(11): 1941-1953.
[2]	BAI Junyan, CAO Heng, WANG Xu, YANG Youbing, FAN Hongdeng, FU Xueyan, SHI Kunpeng, DONG Zhihao, LU Xiaoning, LI Xinyue, HAO Weiguang, LI Ziheng, ZHENG Feiyang. Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep [J]. , 2019, 31(9): 1416-1422.
[3]	LU Xin, ZHOU Jinghang, YANG Chaoyun, ZHANG Menghua, YE Lianmeng, LI Shuzhen, HUANG Xixia, MA Yun, WANG Xingping, SHI Yuangang. Functional annotation of candidate genes for milk production and reproductive traits in Xinjiang Brown cattle [J]. , 2019, 31(12): 1987-1995.
[4]	BAI Junyan, FAN Hongdeng, CAO Heng, PANG Youzhi, SONG Xiaojie, JIANG Mengjuan, FU Xueyan, LU Xiaoning, YANG Shuai, LI Xinyue, HAO Weiguang, LI Ziheng, ZHENG Feiyang. Association analysis between polymorphism of ESR1 gene and egg quality in quail [J]. , 2019, 31(10): 1608-1614.
[5]	JIA Xiaoping, ZHANG Bo, QUAN Jianzhang, WANG Yongfang, DONG Zhiping, YUAN Xilei, LI Jianfeng. Genome-wide association analysis of lodging resistance of millet in Luoyang and Jilin ecological regions [J]. , 2018, 30(12): 1981-1991.
[6]	WANG Hua， WANG Jianfu， WANG Xinrong， CHENG Shuru*， LI Xiaomei， WANG Zhiming， FAN Qingshan， FU Lixia， LI Shuang， ZHOU Xiaoxia. Association analysis of CAST gene polymorphism with meat quality in five sheep breeds [J]. , 2016, 28(8): 1309-.