Diversity of Rubus chingii germplasm resources based on twig and leaf phenotypic traits

doi:10.3969/j.issn.1004-1524.2021.09.10

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (9): 1660-1667.DOI: 10.3969/j.issn.1004-1524.2021.09.10

• Horticultural Science • Previous Articles Next Articles

Diversity of Rubus chingii germplasm resources based on twig and leaf phenotypic traits

HE Qinghai¹(), LIU Bentong¹, ZHOU Zhengde², FANG Ru¹, YANG Shaozong¹^,^*()

1. Zhejiang Academy of Forestry, Hangzhou 310023, China
2. Wuyi Sanhe Tree Plantation, Wuyi 321202, China

Received:2020-08-17 Online:2021-09-25 Published:2021-10-09
Contact: YANG Shaozong

Abstract

Abstract:

To determine the variation degree and law of twig and leaf phenotypic traits in different elite germplasm of Rubus chingii, the phenotypic variations of 10 twig and leaf phenotypic traits of 20 selected accessions were analyzed by variance analysis, principal component analysis and cluster analysis, and the cluster grouping phenotypes were evaluated. The results showed that the phenotypic traits of Rubus chingii varied widely, the coefficient of variation ranged from 10.22% to 46.63%, and the average coefficient of variation was 22.02%. The average coefficient of variation of RC17 and RC01 were 18.79% and 11.27% respectively. Principal component analysis showed that the cumulative contribution rate of the first three principal components was 79.04%, and that of the first principal component was 42.61%.The 20 germplasms of Rubus chingii were divided into 3 groups, including 10 in clusterⅠ, 9 in cluster Ⅱ and 1 in cluster Ⅲ. There were significant (P<0.05) differences in leaf vein angle and lobed number between cluster Ⅰ and cluster Ⅱ. The differences of ground diameter, leaf length, leaf width, petiole length, leaf fissure width and leaf vein angle between cluster Ⅰ and cluster Ⅲ were also significant (P<0.05). The significant (P<0.05) difference was also observed in ground diameter, leaf length, leaf width, petiole length, width of leaf lobes and number of leaf lobes between cluster Ⅱ and cluster Ⅲ. It was concluded that this study would not only provide data basis for resource conservation and core collection construction, but also provide reference for description and efficient utilization of new variety breeding.

Key words: Rubus chingii H.H.Hu, phenotypic traits, germplasm resources, cluster analysis

CLC Number:

S668.9

HE Qinghai, LIU Bentong, ZHOU Zhengde, FANG Ru, YANG Shaozong. Diversity of Rubus chingii germplasm resources based on twig and leaf phenotypic traits[J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1660-1667.

Figures/Tables 6

References 24

[1]	程丹, 李洁, 周斌, 等. 覆盆子化学成分与药理作用研究进展[J]. 中药材, 2012, 35(11):1873-1876.
	CHENG D, LI J, ZHOU B, et al. Studies on chemical constituents and pharmacological action in fruits of Rubus chingii[J]. Journal of Chinese Medicinal Materials, 2012, 35(11):1873-1876.(in Chinese)
[2]	郭启雷, 杨峻山. 掌叶覆盆子的化学成分研究[J]. 中国中药杂志, 2005, 30(3):1141-1143.
	GUO Q L, YANG J S. Studies on chemical constituents in fruit of Rubus chingii[J]. China Journal of Chinese Materia Medica, 2005, 30(3):1141-1143.(in Chinese with English abstract)
[3]	肖洪明, 祖灵博, 李石平, 等. 掌叶覆盆子化学成分的研究[J]. 中国药物化学杂志, 2011, 21(3):220-226.
	XIAO H M, ZU L B, LI S P, et al. Chemical constituents from dried fruits of Rubus chingii[J]. Chinese Journal of Medicinal Chemistry, 2011, 21(3):220-226.(in Chinese with English abstract)
[4]	傅承新, 沈朝栋, 黄爱军. 浙江悬钩子属植物的综合研究:资源调查、引种及开发利用前景[J]. 浙江农业大学学报, 1995, 21(4):393-397.
	FU C X, SHEN C D, HUANG A J. A comprekensive study on Rubus L. in Zhejiang Province: wild specific resources, transplantation and exploltation[J]. Journal of Zhejiang Agricultural University, 1995, 21(4):393-397. (in Chinese)
[5]	潘彬荣, 许立奎, 阮柏苗, 等. 掌叶覆盆子优株的复选及繁殖示范推广研究[J]. 安徽农业科学, 2010, 38(34):19302-19304.
	PAN B R, XU L K, RUAN B M, et al. Study on repeated selection of Rubus chingii Hu superior individual plants and their demonstration and extension[J]. Journal of Anhui Agricultural Sciences, 2010, 38(34):19302-19304.(in Chinese with English abstract)
[6]	汪黑铁. 掌叶覆盆子繁育栽培技术[J]. 现代农业科技, 2019(20):73-74.
	WANG H T. The breeding and cultivation techniques of Rubus chingii[J]. Modern Agricultural Science and Technology, 2019(20):73-74. (in Chinese)
[7]	邬枭楠. 掌叶覆盆子山地种植技术[J]. 现代农业科技, 2016(15):170.
	WU X N. Planting techniques of Rubus chingii in mountainous area[J]. Modern Agricultural Science and Technology, 2016(15):170.(in Chinese)
[8]	游晓庆, 陈慧, 李晓辉, 等. 不同种源掌叶覆盆子种子和果实表型性状及发芽率研究[J]. 南方林业科学, 2019, 47(3):16-19.
	YOU X Q, CHEN H, LI X H, et al. Phenotypic traits and germination rate of Rubus chingii seeds and fruits from different sources[J]. South China Forestry Science, 2019, 47(3):16-19.(in Chinese with English abstract)
[9]	潘彬荣, 许立奎, 阮柏苗, 等. 掌叶覆盆子优株的生长习性调查及选育[J]. 温州农业科技, 2011(1):18-20.
	PAN B R, XU L K, RUAN B M, et al. The growth habit investigation and breeding of Rubus chingii Hu superior individual plants[J]. Wenzhou Agricultural Science and Technology, 2011(1):18-20.(in Chinese)
[10]	张莹, 曹玉芬, 霍宏亮, 等. 基于花表型性状的梨种质资源多样性研究[J]. 园艺学报, 2016, 43(7):1245-1256.
	ZHANG Y, CAO Y F, HUO H L, et al. Research on diversity of pear germplasm resources based on flowers phenotype traits[J]. Acta Horticulturae Sinica, 2016, 43(7):1245-1256.(in Chinese with English abstract)
[11]	王春芳, 余兴华, 王先宏, 等. 基于主要表型性状的不同采集地斑茅种质资源遗传多样性分析[J]. 热带作物学报, 2020, 41(6):1108-1116.
	WANG C F, YU X H, WANG X H, et al. Genetic diversity analysis based on main phenotypic characteristics of Erianthus arundinaceum germplasm from different regions[J]. Chinese Journal of Tropical Crops, 2020, 41(6):1108-1116.(in Chinese with English abstract)
[12]	赵孟良, 韩睿, 田闵玉, 等. 126份芜菁种质资源地上部表型的多样性分析[J]. 分子植物育种, 2021, 19(1):318-332.
	ZHAO M L, HAN R, TIAN M Y, et al. Diversity analysis of aboveground traits of 126 trunip (Brassica rapa var. rapa) germplasm resources[J]. Molecular Plant Breeding, 2021, 19(1):318-332. (in Chinese with English abstract)
[13]	白光红, 张义荣, 刘弋菊, 等. ImageJ图象处理软件在测量玉米子粒大小中的应用[J]. 玉米科学, 2009, 17(1):147-151.
	BAI G H, ZHANG Y R, LIU Y J, et al. Application of ImageJ analysis software in measuring kernel size of maize seeds[J]. Journal of Maize Sciences, 2009, 17(1):147-151.(in Chinese with English abstract)
[14]	宋军生, 党占海, 张建平, 等. 油用亚麻品种资源农艺性状的主成分及聚类分析[J]. 西南农业学报, 2015, 28(2):492-497.
	SONG J S, DANG Z H, ZHANG J P, et al. Principal component analysis and cluster analysis of oil flax germplasm based on agronomic traits[J]. Southwest China Journal of Agricultural Sciences, 2015, 28(2):492-497.(in Chinese with English abstract)
[15]	崔翠, 周清元, 王利鹃, 等. 亚麻种质主要农艺性状主成分分析与综合评价[J]. 西南大学学报(自然科学版), 2016, 38(12):10-18.
	CUI C, ZHOU Q Y, WANG L J, et al. The cluster analysis and comprehensive evaluation of flax germplasm based on principal components of agronomic traits[J]. Journal of Southwest University (Natural Science Edition), 2016, 38(12):10-18.(in Chinese with English abstract)
[16]	张武君, 陈菁瑛, 刘保财, 等. 37份福建山药地方品种主要性状遗传变异研究[J]. 福建农业学报, 2019, 34(11):1246-1254.
	ZHANG W J, CHEN J Y, LIU B C, et al. Genetic variations on major traits of 37 Chinese yam germplasms[J]. Fujian Journal of Agricultural Sciences, 2019, 34(11):1246-1254.(in Chinese with English abstract)
[17]	CUI H L, CHEN C R, HUANG N Z, et al. Association analysis of yield, oil and fatty acid content, and main phenotypic traits in Paeonia rockii as an oil crop[J]. The Journal of Horticultural Science and Biotechnology, 2018, 93(4):425-432. DOI URL
[18]	何庆海, 杨少宗, 李因刚, 等. 枫香树种群种子与果实表型性状变异分析[J]. 植物生态学报, 2018, 42(7):752-763. DOI
	HE Q H, YANG S Z, LI Y G, et al. Phenotypic variations in seed and fruit traits of Liquidambar formosana populations[J]. Chinese Journal of Plant Ecology, 2018, 42(7):752-763.(in Chinese with English abstract) DOI URL
[19]	孙荣喜, 郑勇奇, 张川红, 等. 不同群体国槐种子表型变异研究[J]. 河北农业大学学报, 2011, 34(3):65-70.
	SUN R X, ZHENG Y Q, ZHANG C H, et al. Study on the seed phenotypic variation of Sophora japonica L. in different populations[J]. Journal of Agricultural University of Hebei, 2011, 34(3):65-70.(in Chinese with English abstract)
[20]	刁松锋, 邵文豪, 姜景民, 等. 基于种实性状的无患子天然群体表型多样性研究[J]. 生态学报, 2014, 34(6):1451-1460.
	DIAO S F, SHAO W H, JIANG J M, et al. Phenotypic diversity in natural populationsof Sapindus mukorossi based on fruit and seed traits[J]. Acta Ecologica Sinica, 2014, 34(6):1451-1460.(in Chinese with English abstract)
[21]	张燕, 杨衍, 田丽波, 等. 基于表型性状的苦瓜种质资源评价和遗传多样性的分析[J]. 分子植物育种, 2016, 14(1):239-250.
	ZHANG Y, YANG Y, TIAN L B, et al. Evaluation and genetic diversity of bitter gourd varieties based on phenotypic traits[J]. Molecular Plant Breeding, 2016, 14(1):239-250.(in Chinese with English abstract)
[22]	张怀山, 夏曾润, 代立兰, 等. 中型狼尾草种质资源表型性状的多样性[J]. 西北农业学报, 2014, 23(12):51-59.
	ZHANG H S, XIA Z R, DAI L L, et al. Phenotypic diversity of Pennisetum longissimum var.intermedium germplasm resources[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2014, 23(12):51-59.(in Chinese with English abstract)
[23]	王海平, 李锡香, 沈镝, 等. 基于表型性状的中国大蒜资源遗传多样性分析[J]. 植物遗传资源学报, 2014, 15(1):24-31.
	WANG H P, LI X X, SHEN D, et al. Evaluation on genetic diversity of garlic(Allium sativum L.) clones in China based on morphological characters[J]. Journal of Plant Genetic Resources, 2014, 15(1):24-31.(in Chinese with English abstract)
[24]	芮文婧, 王晓敏, 张倩男, 等. 番茄353份种质资源表型性状遗传多样性分析[J]. 园艺学报, 2018, 45(3):561-570.
	RUI W J, WANG X M, ZHANG Q N, et al. Genetic diversity analysis of 353 tomato germplasm resources by phenotypic traits[J]. Acta Horticulturae Sinica, 2018, 45(3):561-570.(in Chinese with English abstract)

性状 Characters	平均值 Average	标准差 Standard deviation	极差 Range	最小值 Minimum	最大值 Maximum	变异系数 Coefficient of variation/%
株高Plant height/m	1.83	0.28	1.25	1.25	2.50	15.48
地径 Ground diameter/mm	16.47	2.85	14.30	8.70	23.00	17.32
冠幅 Crown breadth/m	1.37	0.35	1.60	0.60	2.20	25.59
分枝数 Branching number	3.06	1.43	6.00	1.00	7.00	46.63
叶片长Leaf length/cm	11.09	1.79	9.32	7.35	16.67	16.17
叶片宽 Leaf width/cm	9.76	2.06	11.30	5.54	16.84	21.15
叶柄长Petiole length/cm	3.74	0.73	4.10	1.95	6.05	19.45
叶裂宽Width of leaf lobes/cm	1.01	0.36	1.97	0.46	2.43	35.36
叶脉角Leaf vein angle/(°)	51.37	5.25	21.89	40.67	62.56	10.22
叶裂数 Number of leaf lobes	5.38	0.69	2.00	4.00	6.00	12.83

性状 Characters	平均值 Average	标准差 Standard deviation	极差 Range	最小值 Minimum	最大值 Maximum	变异系数 Coefficient of variation/%
株高Plant height/m	1.83	0.28	1.25	1.25	2.50	15.48
地径 Ground diameter/mm	16.47	2.85	14.30	8.70	23.00	17.32
冠幅 Crown breadth/m	1.37	0.35	1.60	0.60	2.20	25.59
分枝数 Branching number	3.06	1.43	6.00	1.00	7.00	46.63
叶片长Leaf length/cm	11.09	1.79	9.32	7.35	16.67	16.17
叶片宽 Leaf width/cm	9.76	2.06	11.30	5.54	16.84	21.15
叶柄长Petiole length/cm	3.74	0.73	4.10	1.95	6.05	19.45
叶裂宽Width of leaf lobes/cm	1.01	0.36	1.97	0.46	2.43	35.36
叶脉角Leaf vein angle/(°)	51.37	5.25	21.89	40.67	62.56	10.22
叶裂数 Number of leaf lobes	5.38	0.69	2.00	4.00	6.00	12.83

编号 No.	株高 Plant height	地径 Ground diameter	冠幅 Crown breadth	分枝数 Branching number	叶片长 Leaf length	叶片宽 Leaf width	叶柄长 Petiole length	叶裂宽 Width of leaf lobes	叶脉角 Leaf vein angle	叶裂数 Number of leaf lobes
RC01	8.93	7.98	24.48	34.40	5.81	8.55	9.85	7.48	5.20	0
RC02	6.77	15.57	11.15	24.85	6.50	9.27	10.77	17.03	7.63	8.18
RC03	4.34	16.02	19.23	25.91	14.13	16.77	13.96	46.93	6.24	5.31
RC04	16.12	18.82	21.13	31.62	6.63	7.00	12.06	17.05	7.09	10.90
RC05	11.37	10.56	32.32	38.03	11.06	17.24	16.39	16.71	8.51	12.99
RC06	8.74	4.18	18.12	81.31	5.13	4.40	17.41	33.05	7.92	2.26
RC07	14.44	18.38	17.56	39.49	16.91	12.08	24.76	21.82	12.57	9.91
RC08	14.52	10.68	19.93	38.03	7.71	16.76	9.98	38.08	11.88	9.30
RC09	15.06	8.86	19.93	39.53	15.75	20.34	25.05	14.94	8.77	19.37
RC10	6.76	4.96	23.96	39.12	7.09	14.38	8.19	14.08	7.15	0.00
RC11	11.90	16.13	34.05	33.33	12.93	12.94	16.15	15.14	9.66	18.69
RC12	3.89	8.90	25.49	23.57	12.51	12.17	19.21	24.07	6.57	12.57
RC13	7.28	12.66	16.13	47.51	3.11	7.17	1.46	16.57	8.46	5.08
RC14	7.89	22.76	21.63	34.23	13.38	15.76	21.63	33.85	4.77	9.91
RC15	5.54	29.06	24.09	26.31	10.64	23.30	13.14	13.11	8.43	10.65
RC16	7.47	5.28	13.56	30.62	15.98	14.29	16.50	11.58	8.31	8.44
RC17	7.60	9.74	20.22	22.82	14.79	12.59	17.90	18.82	7.76	8.60
RC18	9.10	6.46	19.93	60.11	5.04	4.30	6.20	21.13	7.62	5.36
RC19	4.87	8.23	18.92	39.12	16.55	15.41	20.47	19.59	5.23	3.99
RC20	9.41	14.29	17.12	23.57	6.31	9.09	15.92	21.35	15.92	8.44
平均值Average	9.10	12.48	20.95	36.67	10.40	12.69	14.85	21.12	8.29	8.50

编号 No.	株高 Plant height	地径 Ground diameter	冠幅 Crown breadth	分枝数 Branching number	叶片长 Leaf length	叶片宽 Leaf width	叶柄长 Petiole length	叶裂宽 Width of leaf lobes	叶脉角 Leaf vein angle	叶裂数 Number of leaf lobes
RC01	8.93	7.98	24.48	34.40	5.81	8.55	9.85	7.48	5.20	0
RC02	6.77	15.57	11.15	24.85	6.50	9.27	10.77	17.03	7.63	8.18
RC03	4.34	16.02	19.23	25.91	14.13	16.77	13.96	46.93	6.24	5.31
RC04	16.12	18.82	21.13	31.62	6.63	7.00	12.06	17.05	7.09	10.90
RC05	11.37	10.56	32.32	38.03	11.06	17.24	16.39	16.71	8.51	12.99
RC06	8.74	4.18	18.12	81.31	5.13	4.40	17.41	33.05	7.92	2.26
RC07	14.44	18.38	17.56	39.49	16.91	12.08	24.76	21.82	12.57	9.91
RC08	14.52	10.68	19.93	38.03	7.71	16.76	9.98	38.08	11.88	9.30
RC09	15.06	8.86	19.93	39.53	15.75	20.34	25.05	14.94	8.77	19.37
RC10	6.76	4.96	23.96	39.12	7.09	14.38	8.19	14.08	7.15	0.00
RC11	11.90	16.13	34.05	33.33	12.93	12.94	16.15	15.14	9.66	18.69
RC12	3.89	8.90	25.49	23.57	12.51	12.17	19.21	24.07	6.57	12.57
RC13	7.28	12.66	16.13	47.51	3.11	7.17	1.46	16.57	8.46	5.08
RC14	7.89	22.76	21.63	34.23	13.38	15.76	21.63	33.85	4.77	9.91
RC15	5.54	29.06	24.09	26.31	10.64	23.30	13.14	13.11	8.43	10.65
RC16	7.47	5.28	13.56	30.62	15.98	14.29	16.50	11.58	8.31	8.44
RC17	7.60	9.74	20.22	22.82	14.79	12.59	17.90	18.82	7.76	8.60
RC18	9.10	6.46	19.93	60.11	5.04	4.30	6.20	21.13	7.62	5.36
RC19	4.87	8.23	18.92	39.12	16.55	15.41	20.47	19.59	5.23	3.99
RC20	9.41	14.29	17.12	23.57	6.31	9.09	15.92	21.35	15.92	8.44
平均值Average	9.10	12.48	20.95	36.67	10.40	12.69	14.85	21.12	8.29	8.50

性状 Characters	主成分载荷矩阵Principal component load matrix			主成分特征向量Principal component eigenvector
性状 Characters	1	2	3	1	2	3
株高Plant hight	0.24	0.59	0.56	0.06	0.29	0.35
地径 Ground diameter	0.71	0.31	-0.06	0.17	0.15	-0.04
冠幅 Crown breadth	0.38	0.64	-0.50	0.09	0.31	-0.31
分枝数 Branching number	0.24	0.77	-0.31	0.06	0.37	-0.20
叶片长 length of leaf	0.96	-0.04	0.14	0.23	-0.02	0.09
叶片宽 Width of leaf	0.95	-0.21	0.12	0.22	-0.10	0.08
叶柄长Length of petiole	0.83	0.15	0.05	0.20	0.07	0.03
叶裂宽 Lplit width of leaf	0.68	-0.48	0.38	0.16	-0.23	0.24
叶脉角 Vein angle	-0.14	0.38	0.78	-0.03	0.19	0.49
叶裂数 Split number of leaf	0.70	-0.42	-0.37	0.17	-0.20	-0.23

Diversity of Rubus chingii germplasm resources based on twig and leaf phenotypic traits

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 24

Related Articles 15

Recommended Articles

Metrics

Comments

成分 Components	初始特征值Initial eigenvalue			主成分特征值Principal component eigenvalu
成分 Components	特征值 Eigenvalue	方差贡献率 Variance contribution rate/%	累积方差贡献率 Cumulative variance contribution rate/%	特征值 Eigenvalue	方差贡献率 Variance contribution rate/%	累积方差贡献率 Cumulative variance contribution rate/%
1	4.26	42.61	42.61	4.26	42.61	42.61
2	2.05	20.52	63.13	2.05	20.52	63.13
3	1.59	15.91	79.04	1.59	15.91	79.04
4	0.87	8.74	87.78
5	0.44	4.43	92.21

性状Characters	第Ⅰ类ClusterⅠ	第Ⅱ类ClusterⅡ	第Ⅲ类Cluster Ⅲ
株高Plant height/m	1.76±0.27 a	1.91±0.30 a	1.86±0.17 a
地径 Ground diameter/mm	17.00±2.55 b	15.65 ±3.01 b	19.20 ±1.53 a
冠幅 Crown breadth/m	1.43 ±0.34 a	1.30±0.36 a	1.30±0.32 a
分枝数 Branching number	3.02±1.56 a	3.13 ±1.32 a	2.60±0.89 a
叶片长Length of leaf/cm	11.10±1.53 b	10.67±1.55 b	15.41±0.90 a
叶片宽 Width of leaf/cm	9.88±1.68 b	9.13±1.74 b	15.33±1.31 a
叶柄长Length of petiole/cm	3.72±0.72 b	3.63±0.63 b	4.99±0.49 a
叶裂宽 Split width of leaf/cm	1.00±0.29 b	0.95±0.34 b	1.91±0.14 a
叶脉角 Vein angle/(°)	48.31±4.64 b	54.51±3.99 a	53.19±2.77 a
叶裂数 Split number of leaf	5.61±0.59 a	5.08±0.70 b	6.00±0 a

[1]	WANG Zhiqi, SUN Jian, LIANG Junchao, ZHAO Yunyan, YAN Tingxian, YAN Xiaowen, WEI Wenliang, LE Meiwang. Study on genetic diversity of sesame germplasm in Jiangxi Province based on molecular markers [J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1565-1580.
[2]	ZHANG Jingzhen, WANG Lianjun, LEI Jian, CHAI Shasha, YANG Xinsun, ZHANG Wenying. Genetic diversity analysis and construction of DNA fingerprint of yam (Dioscorea oppositeac Thunb.) germplasm by cpSSR marker [J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1222-1233.
[3]	ZHAI Xiuming, LI Jie, TANG Min, HU Fangjie, ZHANG Jun, HOU Yujia, XU Ze. Diversity analysis of 30 tea (Camelia sinensis) germplasm resources in Chongqing based on agronomic traits and biochemical components [J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1244-1255.
[4]	REN Qianqian, ZHANG Jingwei, SUN Jixia, LUO Zhenzhen, DING Zhaotang, ZHANG Deshun, ZHANG Yingjie, GUO Duitian, GUO Wenjiao. Analysis and comprehensive evaluation of 25 Hydrangea germplasm resources [J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1012-1024.
[5]	ZHENG Junqing, LI Ruiyuan, ZHENG Ran, LYU Dan, CHEN Qijiao, SHI Taoxiong, CHEN Qingfu. Variation analysis of grain weight, grain shape and protein content in recombinant inbred lines population of tartary buckwheat [J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 565-575.
[6]	ZHAO Ke, LI Qiurong, HOU Lu, BAI Yaobo, JIANG Liling, WEI Youhai, GUO Qingyun. Genetic analysis of stripe rust resistance genes in 2 spring wheat germplasm resources at adult stage [J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 595-601.
[7]	ZHANG Ting, LIU Huiqin, GUO Qinwei, LI Chaosen, ZHANG Xinhui, XIANG Xiaomin, ZHAO Dongfeng, WAN Hongjian. Principal component analysis and cluster analysis for evaluating free amino acids of 16 pepper materials [J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 640-650.
[8]	XIE Ziyu, WANG Ke’er, ZHAO Wenliang, WEN Zuhui, CHEN Linrun, XU Lishan. Nutritional components and bioactivities of sweet potatoes with different flesh colors [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 183-192.
[9]	HONG Xia, ZHAO Yongbin, QU Weidong, CHEN Yinlong, QIU Liping, WANG Jiaoyang. Comparative analysis on genetic diversity of Colocasia esculenta germplasm in Zhejiang Province based on phenotype and simple sequence repeats markers [J]. , 2020, 32(9): 1544-1554.
[10]	SHEN Di, CHEN Longzheng, LU Xiaohua, TAO Jianping, FENG Gucheng, LIU Jiexia, FENG Kai, YIN Lian, DING Xu, JIA Lili, XU Zhisheng, LIU Huiji, XIONG Aisheng. Resources evaluation of 29 celery varieties for autumn-winter cultivation in southern Jiangsu [J]. , 2020, 32(4): 653-660.
[11]	SHEN Shengfa, XIANG Chao, WU Liehong, LI Bing, LUO Zhigao. Determination and difference analysis of soluble sugar content in 11 sweetpotato germplasm resources [J]. , 2020, 32(11): 1934-1940.
[12]	MA Xiaohua, YU Zheping, ZHENG Xiliang, HU Xiaojin, ZHANG Shuwen, QI Xingjiang, MA Jingyan. Introduction experiment and phenotype cluster analysis of Chinese bayberry in Jingzhou [J]. , 2020, 32(11): 1987-1993.
[13]	XIE Wengang, LI Xiaosong, LI Wei, TANG Qian. Genetic diversity of “Small & Medium Leaf” tea resources in Sichuan Province based on phenotypic characteristics [J]. , 2019, 31(9): 1405-1415.
[14]	XU Xiao, LUAN Haiye, ZHANG Yinghu, LU Jian, QIAO Hailong, ZANG Hui, YANG Hongyan, SHEN Huiquan. Morphological diversity of hulless barley accessions from Qinghai-Tibetan Plateau [J]. , 2019, 31(7): 1037-1044.
[15]	LI Mingyu, WANG Yan, LIANG Danni, YAO Yani, LAN Jian. Comprehensive evaluation of salt tolerance of 22 alfalfa germplasms at germination stage [J]. , 2019, 31(5): 746-755.