Acute toxicity and risk assessment of neonicotinoid insecticides to the silkworm, Bombyx mori

doi:10.3969/j.issn.1004-1524.2021.10.17

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (10): 1931-1938.DOI: 10.3969/j.issn.1004-1524.2021.10.17

• Quality and Safety of Agricultural Products • Previous Articles Next Articles

Acute toxicity and risk assessment of neonicotinoid insecticides to the silkworm, Bombyx mori

LIU Xinju(), WU Shenggan, AN Xuehua, JIANG Jinhua, LYU Lu, LI Gang, WANG Feidi, ZHAO Xueping^*()

Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, State Key Laboratory for Managing Biotic and Chemical Threats to Quality and Safety of Agro-products, Hangzhou 310021,China

Received:2021-01-12 Online:2021-10-25 Published:2021-11-02
Contact: ZHAO Xueping

Abstract

Abstract:

In order to provide a scientific basis for the rational use of neonicotinoid insecticides, the acute toxicity of six neonicotinoid insecticides and other 2 kinds of control insecticides to the silkworm (Bombyx mori) were determined by using leaf dipping method, and the risk assessment was also carried out according to the risk quotient method. Results exhibited that the 96 h-LC₅₀ values of acetamiprid and clothianidin to B. mori were 0.262 and 0.268 mg·L^-1, respectively, which belonged to extremely toxic grade. The 96 h-LC₅₀ values of thiacloprid, imidacloprid, dinotefuran and thiamethoxam to the organisms were 0.582, 1.68, 2.60 and 2.16 mg·L^-1, respectively, which belonged to highly toxic grade. The 96 h-LC₅₀ values of lambda-cyhalothrin, chlorpyrifos and malathion to the silkworms were 0.009 09, 0.785 and 1 072 mg·L^-1, respectively, which belonged to extremely toxic grade, highly toxic grade and lowly toxic grade, respectively. Risk assessment results indicated that the risk of malathion to the silkworm was acceptable, and the other eight insecticides were unacceptable. Therefore, neonicotinoid insecticides should be used cautiously in integrated pest management to avoid harm for sericulture.

Key words: neonicotinoid insecticides, silkworm, acute toxicity, risk assessment

CLC Number:

S881

LIU Xinju, WU Shenggan, AN Xuehua, JIANG Jinhua, LYU Lu, LI Gang, WANG Feidi, ZHAO Xueping. Acute toxicity and risk assessment of neonicotinoid insecticides to the silkworm, Bombyx mori[J]. Acta Agriculturae Zhejiangensis, 2021, 33(10): 1931-1938.

Figures/Tables 5

References 26

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药剂种类与名称 Category and name of the agent		毒性回归方程 Toxicity regression equation	LC₅₀/ (mg·L^-1)	95% 置信区间 95% Confidence interval/(mg·L^-1)
新烟碱类Neonicotinoid	啶虫脒Acetamiprid	Y=8.975+6.807X	0.262	0.246~0.275
	噻虫胺Clothianidin	Y =10.356+9.327X	0.268	0.251~0.288
	噻虫啉Thiacloprid	Y =5.808+3.437X	0.582	0.518~0.674
	吡虫啉Imidacloprid	Y=3.199+7.952X	1.68	1.61~1.77
	呋虫胺Dinotefuran	Y=-0.926+14.247X	2.60	2.54~2.70
	噻虫嗪Thiamethoxam	Y =2.202+8.737X	2.16	1.85~2.87
有机磷类Organophosphate class	马拉硫磷Malathion	Y=-8.414+4.426X	1072	985~1186
	毒死蜱Chlorpyrifos	Y=5.577+5.504X	0.785	0.739~0.833
拟除虫菊酯类Pyrethroid class	高效氯氟氰菊酯Lambda-cyhalothrin	Y =16.267+5.520X	0.009 09	0.008 52~0.009 77

药剂种类与名称 Category and name of the agent		毒性回归方程 Toxicity regression equation	LC₅₀/ (mg·L^-1)	95% 置信区间 95% Confidence interval/(mg·L^-1)
新烟碱类Neonicotinoid	啶虫脒Acetamiprid	Y=8.975+6.807X	0.262	0.246~0.275
	噻虫胺Clothianidin	Y =10.356+9.327X	0.268	0.251~0.288
	噻虫啉Thiacloprid	Y =5.808+3.437X	0.582	0.518~0.674
	吡虫啉Imidacloprid	Y=3.199+7.952X	1.68	1.61~1.77
	呋虫胺Dinotefuran	Y=-0.926+14.247X	2.60	2.54~2.70
	噻虫嗪Thiamethoxam	Y =2.202+8.737X	2.16	1.85~2.87
有机磷类Organophosphate class	马拉硫磷Malathion	Y=-8.414+4.426X	1072	985~1186
	毒死蜱Chlorpyrifos	Y=5.577+5.504X	0.785	0.739~0.833
拟除虫菊酯类Pyrethroid class	高效氯氟氰菊酯Lambda-cyhalothrin	Y =16.267+5.520X	0.009 09	0.008 52~0.009 77

药剂名称 Drug name	作物 Crop	AR/(kg· hm^-2)	施药次数 Application times	施药间隔期 Application interval	M_AF	D_PHI	PEC_ma-fr/ (mg·kg^-1)	PEC_ma-sr/ (mg·kg^-1)
啶虫脒Acetamiprid	黄瓜Cucumber	0.090 0	2	7	1.616	0.933	12.6	0.773
噻虫胺Clothianidin	水稻Rice	0.150 0	2	7	1.616	0.933	21.1	1.289
噻虫啉Thiacloprid	水稻Rice	0.123 0	2	7	1.616	0.933	17.3	1.057
吡虫啉Imidacloprid	小麦Wheat	0.150 0	2	3	1.812	0.933	23.6	1.446
呋虫胺Dinotefuran	水稻Rice	0.150 0	2	5	1.707	0.933	22.2	1.362
噻虫嗪Thiamethoxam	水稻Rice	0.078 8	2	5	1.707	0.933	11.7	0.715
高效氯氟氰菊酯	棉花Cotton	0.030 0	3	7	1.995	0.933	5.20	0.328
Lambda-cyhalothrin
毒死蜱Chlorpyrifos	棉花Cotton	1.050 0	2	7	1.616	0.933	147	9.020
马拉硫磷Malathion	十字花科蔬菜	0.810 0	2	7	1.616	0.933	114	6.960
	Brassicaceous vegetable

药剂名称 Drug name	作物 Crop	AR/(kg· hm^-2)	施药次数 Application times	施药间隔期 Application interval	M_AF	D_PHI	PEC_ma-fr/ (mg·kg^-1)	PEC_ma-sr/ (mg·kg^-1)
啶虫脒Acetamiprid	黄瓜Cucumber	0.090 0	2	7	1.616	0.933	12.6	0.773
噻虫胺Clothianidin	水稻Rice	0.150 0	2	7	1.616	0.933	21.1	1.289
噻虫啉Thiacloprid	水稻Rice	0.123 0	2	7	1.616	0.933	17.3	1.057
吡虫啉Imidacloprid	小麦Wheat	0.150 0	2	3	1.812	0.933	23.6	1.446
呋虫胺Dinotefuran	水稻Rice	0.150 0	2	5	1.707	0.933	22.2	1.362
噻虫嗪Thiamethoxam	水稻Rice	0.078 8	2	5	1.707	0.933	11.7	0.715
高效氯氟氰菊酯	棉花Cotton	0.030 0	3	7	1.995	0.933	5.20	0.328
Lambda-cyhalothrin
毒死蜱Chlorpyrifos	棉花Cotton	1.050 0	2	7	1.616	0.933	147	9.020
马拉硫磷Malathion	十字花科蔬菜	0.810 0	2	7	1.616	0.933	114	6.960
	Brassicaceous vegetable

药剂名称Drug name	F_C/(L·kg^-1)	LC₅₀-_C/(mg·kg^-1)	F_U	C_PNE/(mg·kg^-1)
啶虫脒Acetamiprid	0.46	0.121	70	0.001 73
噻虫胺Clothianidin	0.46	0.123	70	0.001 76
噻虫啉Thiacloprid	0.46	0.268	70	0.003 83
吡虫啉Imidacloprid	0.46	0.773	70	0.011 0
呋虫胺Dinotefuran	0.46	1.196	70	0.017 10
噻虫嗪Thiamethoxam	0.46	0.994	70	0.014 20
高效氯氟氰菊酯Lambda-cyhalothrin	0.46	0.004 18	70	0.000 0597
毒死蜱Chlorpyrifos	0.46	0.361	70	0.005 16
马拉硫磷Malathion	0.46	493	70	7.040 00

Acute toxicity and risk assessment of neonicotinoid insecticides to the silkworm, Bombyx mori

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药剂名称Drug name	RQ_fr	风险评估Risk assessment	RQ_sr	风险评估Risk assessment
啶虫脒Acetamiprid	7 283	不可接受Unacceptable	447	不可接受Unacceptable
噻虫胺Clothianidin	11 989	不可接受Unacceptable	732	不可接受Unacceptable
噻虫啉Thiacloprid	4 517	不可接受Unacceptable	276	不可接受Unacceptable
吡虫啉Imidacloprid	2 145	不可接受Unacceptable	131	不可接受Unacceptable
呋虫胺Dinotefuran	1 298	不可接受Unacceptable	79.6	不可接受Unacceptable
噻虫嗪Thiamethoxam	824	不可接受Unacceptable	50.4	不可接受Unacceptable
高效氯氟氰菊酯Lambda-cyhalothrin	87 102	不可接受Unacceptable	5 494	不可接受Unacceptable
毒死蜱Chlorpyrifos	28 488	不可接受Unacceptable	1 748	不可接受Unacceptable
马拉硫磷Malathion	16.2	不可接受Unacceptable	0.989	可接受Acceptable

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