Degradation of carbosulfan after its application in different growth stage of cowpea

doi:10.3969/j.issn.1004-1524.2020.11.16

›› 2020, Vol. 32 ›› Issue (11): 2050-2058.DOI: 10.3969/j.issn.1004-1524.2020.11.16

• Quality and Safety of Agriculturel Products • Previous Articles Next Articles

Degradation of carbosulfan after its application in different growth stage of cowpea

WANG Di^1,2, DI Shanshan¹, WANG Xinquan¹, ZHANG Changpeng¹, WANG Xiangyun^1,*, WANG Meng^3,*

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products (in Prepared), Key Laboratory for Pesticide Residue Detection, Ministry of Agriculture and Rural Affairs, Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Horticulture, Hainan University, Haikou 570228, China;
3. College of Plant Protection, Hainan University, Haikou 570228, China

Received:2020-06-07 Online:2020-11-25 Published:2020-12-02

Abstract

Abstract: The degradation and risk assessment of carbosulfan application in different growth stages of cowpea (sowing, seedling and podding) were investigated. In field experiments, carbosulfan EC (emulsifiable concentrate) was applied in the different growth stages of cowpea with different doses (low dose, high dose and 1.5-fold high dose according to the registration). Cowpea samples were collected at mature stage, which were extracted with acetonitrile and purified by C18. The residue levels of carbosulfan and its metabolites (carbofuran and 3-hydroxycarbofuran) were determined by LC-MS/MS (liquid chromatography-tandem mass spectrometry). The limit of quantification (LOQ) values of carbosulfan, carbofuran and 3-hydroxycarbofuran were all 0.01 mg·kg^-1. The average recoveries of carbosulfan and its metabolites in cowpea at 4 spiked levels (0.01, 0.05, 0.2, 1 mg·kg^-1) ranged from 72% to 105% with relative standard deviation (RSD) in the range of 1.4%-20.1%. The residue risk of carbosulfan was mainly due to its metabolites (carbofuran and 3-hydroxycarbofuran). When carbosulfan was applied at sowing stage of cowpea, the residue levels of carbosulfan and its metabolites were below LOQ. After application at 1.5-fold high dose in the seedling stage, the residue level of carbofuran (including 3-hydroxycarbofuran) in cowpea on the 10th day exceeded the maximum residue limit (MRL) in China. When carbosulfan was applied in the podding stage two or three times, the residue level of carbofuran (including 3-hydroxycarbofuran) in cowpea within 7 d exceeded the MRL. When carbosulfan was applied in the podding stage of cowpea, the risk of chronic dietary intake and acute dietary intake of carbosulfan was lower than 100%, but the risk of acute dietary intake of carbofuran (including 3-hydroxycarbofuran) was higher than 100% within 7 d. Therefore, the application of carbosulfan in the sowing stage of cowpea was recommended, while application in the seedling and podding stage existed high risk, which should be forbiden.

Key words: cowpea, carbosulfan, carbofuran, 3-hydroxycarbofuran, pesticide residue, risk assessment

CLC Number:

WANG Di, DI Shanshan, WANG Xinquan, ZHANG Changpeng, WANG Xiangyun, WANG Meng. Degradation of carbosulfan after its application in different growth stage of cowpea[J]. , 2020, 32(11): 2050-2058.

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Degradation of carbosulfan after its application in different growth stage of cowpea

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