Screening of cowpea genotypes with high-frequency regeneration in Agrobacterium-mediated transformation

doi:10.3969/j.issn.1004-1524.2018.09.07

›› 2018, Vol. 30 ›› Issue (9): 1489-1495.DOI: 10.3969/j.issn.1004-1524.2018.09.07

• Horticultural Science • Previous Articles Next Articles

Screening of cowpea genotypes with high-frequency regeneration in Agrobacterium-mediated transformation

ZHOU Wen^{1, 2}, WU Xinyi², WANG Baogen², WU Xiaohua², LU Zhongfu², WANG Ying², XU Pei², LI Guojing^{2, *}

1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321000, China;
2. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-01-15 Online:2018-09-25 Published:2018-10-15
Contact: 李国景,E-mail: ligj@mail.zaas.ac.cn
Supported by:
“十三五”国家重点研发计划(2016YFD0100204-32); 浙江省公益性项目(LGN18C150009); 浙江省农业新品种选育重大科技专项“蔬菜新品种选育”领域(2016C02051-7-3); 国家自然科学基金(31572135,31772299); 浙江省自然科学基金(LY15C150002)

Abstract

Abstract: In this study, 51 cowpea genotypes were tested for tissue culture and shoot induction in Agrobacterium-mediated transformation. After a preliminary screening for regeneration, the rate of shoot regeneration was graded and 10 genotypes with a regeneration rate above 80% were tested further in three replicates. White August Cowpea was found to show the highest reproduction coefficient and induction rate of adventitious buds, which were 1.93 and 86.40%, respectively. Therefore, this genotype may be used as a good explant source in the future transgenic studies.

Key words: cowpea, genotype, Agrobacterium tumefaciens, regeneration rate

CLC Number:

S643.4

ZHOU Wen, WU Xinyi, WANG Baogen, WU Xiaohua, LU Zhongfu, WANG Ying, XU Pei, LI Guojing. Screening of cowpea genotypes with high-frequency regeneration in Agrobacterium-mediated transformation[J]. , 2018, 30(9): 1489-1495.

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Screening of cowpea genotypes with high-frequency regeneration in Agrobacterium-mediated transformation

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