Optimization of Chinese medicine plant extract compounds with orthogonal design

doi:10.3969/j.issn.1004-1524.2021.12.09

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2295-2303.DOI: 10.3969/j.issn.1004-1524.2021.12.09

• Animal Science • Previous Articles Next Articles

Optimization of Chinese medicine plant extract compounds with orthogonal design

TAO Xin(), WU Wanyun, SUN Yuqing, MEN Xiaoming, XU Ziwei^*()

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-06-28 Online:2021-12-25 Published:2022-01-10
Contact: XU Ziwei

Abstract

Abstract:

To prepare Chinese medicine plant extracts replacing feed antibiotics. Firstly, Grana Pericarpium, Cinnamomi Ramulus, Magnoliae officinalis cortex, Artemisiae annuae herba, Spatholobi caulis were sieved from 19 Chinese medicine plants as materials of extract compounds by examining antibacterial rate, inhibitory rateanti-inflammatory rate, and antioxidant IC₅₀ value as evaluating index. Secondly, proportions of five materials were optimized by a L₁₆ (4 ⁵) orthogonal experiment,by range analysis of results, three extract compounds with the strongest antibacterial (F_ab), anti-inflammatory (F_ai) and antioxidant (F_ao) activities were obtained. These material ratios of Magnoliae officinalis cortex, Spatholobi caulis, Artemisiae annuae herba, Cinnamomi Ramulus and Grana Pericarpium were 7∶13∶6∶9∶1 in F_ab, 7∶13∶4∶1∶1 in F_ai, and 3∶13∶4∶1∶7 in F_ao, respectively. Finally, the verifying results showed that the minimum inhibitory concentration of F_ab was 125 mg·mL^-1, the anti-inflammatory IC₅₀ value of F_ai was 0.144 mg·mL^-1, and the antioxidant IC₅₀ value of F_ao was 0.222 mg·mL^-1. In summary, the present study obtained the best three Chinese medicine extract compounds with stronger antibacterial, anti-inflammatory and antioxidant effects, respectively.

Key words: Chinese medicine, orthogonal design, plant extract compounds, antibacterial, anti-inflammatory, antioxidant

CLC Number:

S853.7

TAO Xin, WU Wanyun, SUN Yuqing, MEN Xiaoming, XU Ziwei. Optimization of Chinese medicine plant extract compounds with orthogonal design[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2295-2303.

Figures/Tables 9

References 19

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水平 Level	因素 Factors
水平 Level	A厚朴 Magnoliae officinalis cortex	B鸡血藤 Spatholobi caulis	C青蒿 Artemisiae annuae herba	D桂枝 Cinnamomi Ramulus	E石榴皮 Grana Pericarpium
1	1	7	4	1	1
2	3	9	6	3	3
3	5	11	8	6	5
4	7	13	10	9	7

水平 Level	因素 Factors
水平 Level	A厚朴 Magnoliae officinalis cortex	B鸡血藤 Spatholobi caulis	C青蒿 Artemisiae annuae herba	D桂枝 Cinnamomi Ramulus	E石榴皮 Grana Pericarpium
1	1	7	4	1	1
2	3	9	6	3	3
3	5	11	8	6	5
4	7	13	10	9	7

试验号 Number	因素Factors					评价指标Evaluation index
试验号 Number	A	B	C	D	E	抑菌率 Antibacterial rate/%	抗炎率 Anti-inflammatoryrate/%	抗氧化IC₅₀值 Antioxidant IC₅₀value/(mg·mL^-1)
1	1	1	1	1	1	34.53±5.988	86.90±2.755	0.817±0.039
2	1	2	2	2	2	33.73±2.762	78.49±2.425	0.822±0.043
3	1	3	3	3	3	9.370±1.931	69.54±1.091	0.682±0.027
4	1	4	4	4	4	36.53±4.651	75.06±1.655	0.395±0.026
5	2	1	2	3	4	26.95±4.262	57.16±1.137	0.293±0.018
6	2	2	1	4	3	31.74±4.383	73.06±3.190	0.272±0.018
7	2	3	4	1	2	17.82±3.835	79.89±0.825	0.481±0.039
8	2	4	3	2	1	38.52±9.606	94.32±0.689	0.695±0.021
9	3	1	3	4	2	59.08±3.609	72.19±0.852	0.726±0.035
10	3	2	4	3	1	79.44±8.643	91.77±0.369	2.011±0.056
11	3	3	1	2	4	8.908±3.602	81.67±2.451	0.335±0.028
12	3	4	2	1	3	79.64±7.162	92.81±0.995	0.266±0.031
13	4	1	4	2	3	60.48±5.215	73.47±0.730	0.288±0.012
14	4	2	3	1	4	28.54±1.521	79.68±0.705	0.245±0.032
15	4	3	2	4	1	81.04±5.463	93.47±0.521	1.090±0.037
16	4	4	1	3	2	71.06±5.624	97.53±1.587	0.722±0.029

试验号 Number	因素Factors					评价指标Evaluation index
试验号 Number	A	B	C	D	E	抑菌率 Antibacterial rate/%	抗炎率 Anti-inflammatoryrate/%	抗氧化IC₅₀值 Antioxidant IC₅₀value/(mg·mL^-1)
1	1	1	1	1	1	34.53±5.988	86.90±2.755	0.817±0.039
2	1	2	2	2	2	33.73±2.762	78.49±2.425	0.822±0.043
3	1	3	3	3	3	9.370±1.931	69.54±1.091	0.682±0.027
4	1	4	4	4	4	36.53±4.651	75.06±1.655	0.395±0.026
5	2	1	2	3	4	26.95±4.262	57.16±1.137	0.293±0.018
6	2	2	1	4	3	31.74±4.383	73.06±3.190	0.272±0.018
7	2	3	4	1	2	17.82±3.835	79.89±0.825	0.481±0.039
8	2	4	3	2	1	38.52±9.606	94.32±0.689	0.695±0.021
9	3	1	3	4	2	59.08±3.609	72.19±0.852	0.726±0.035
10	3	2	4	3	1	79.44±8.643	91.77±0.369	2.011±0.056
11	3	3	1	2	4	8.908±3.602	81.67±2.451	0.335±0.028
12	3	4	2	1	3	79.64±7.162	92.81±0.995	0.266±0.031
13	4	1	4	2	3	60.48±5.215	73.47±0.730	0.288±0.012
14	4	2	3	1	4	28.54±1.521	79.68±0.705	0.245±0.032
15	4	3	2	4	1	81.04±5.463	93.47±0.521	1.090±0.037
16	4	4	1	3	2	71.06±5.624	97.53±1.587	0.722±0.029

因素 Factor	抑菌率 Antibacterial rate						抗炎率 Anti-inflammatory rate						抗氧化IC50值 Antioxidant IC50 value
因素 Factor	k₁/ %	k₂/ %	k₃/ %	k₄/ %	R/ %	最优水平 The best level	k₁/ %	k₂/ %	k₃/ %	k₄/ %	R/ %	最优水平 The best level	k₁/ (mg· mL^-1)	k₂/ (mg· mL^-1)	k₃/ (mg· mL^-1)	k₄/ (mg· mL^-1)	R/ (mg· mL^-1)	最优水平 The best level
A	28.54	28.76	56.77	60.28	31.74	4	77.50	76.11	84.61	86.79	9.929	4	0.679	0.435	0.835	0.587	0.4	2
B	45.26	43.36	29.29	56.44	27.15	4	72.43	80.75	81.14	89.93	17.50	4	0.531	0.838	0.647	0.519	0.319	4
C	36.56	55.34	33.88	48.57	21.46	2	84.79	80.48	78.93	80.05	5.857	1	0.536	0.618	0.587	0.794	0.258	1
D	40.13	35.41	46.71	52.1	16.69	4	84.82	81.99	79.00	78.45	6.375	1	0.452	0.535	0.927	0.621	0.475	1
E	58.38	45.42	45.31	25.23	33.15	1	91.62	82.03	77.22	73.39	18.22	1	1.153	0.688	0.377	0.317	0.836	4

Optimization of Chinese medicine plant extract compounds with orthogonal design

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变异来源	自由度	均方	F值	P值
Sources of variation	Degree of freedom	Mean square	F value	P value
抑菌率 Antibacterial rate
A	3	3 594.26	126.06	<0.001
B	3	894.22	31.36	<0.001
C	3	1 226.28	43.01	<0.001
D	3	1 438.99	50.47	<0.001
E	3	2 834.09	99.40	<0.001
误差Error	32	28.51	—	—
抗炎率 Anti-inflammatory rate
A	3	298.33	114.46	<0.001
B	3	574.09	220.26	<0.001
C	3	79.14	30.36	<0.001
D	3	65.26	25.04	<0.001
E	3	577.70	221.64	<0.001
误差Error	32	2.606	—	—
抗氧化IC₅₀值 Antioxidant IC₅₀ value
A	3	0.336	318.64	<0.001
B	3	0.114	108.29	<0.001
C	3	0.150	142.26	<0.001
D	3	0.586	555.44	<0.001
E	3	1.458	1 383.4	<0.001
误差Error	32	0.001	—	—

因素 Factor	水平 Level	评价指标Evaluation index
因素 Factor	水平 Level	抑菌率 Antibacterial rate%	抗炎率 Anti-inflammatory rate%	抗氧化IC50值 Antioxidant IC50 value/(mg·mL^-1)
A	1	28.54±1.541 b	77.50±0.446 b	0.679±0.009 b
	2	28.76±1.541 b	76.11±0.446 c	0.435±0.009 b
	3	56.77±1.541 a	87.11±0.446 a	0.834±0.009 d
	4	60.28±1.541 a	86.04±0.446 a	0.586±0.009 c
B	1	40.26±1.748 b	72.08±0.528 c	0.577±0.011 c
	2	40.13±1.748 b	80.49±0.528 b	0.753±0.011 a
	3	37.51±1.748 b	81.75±0.528 b	0.685±0.011 b
	4	56.44±1.541 a	89.93±0.466 a	0.519±0.009 d
C	1	36.56±1.541 c	84.79±0.466 a	0.536±0.009 c
	2	63.57±2.101 a	81.09±0.635 b	0.656±0.013 b
	3	30.65±2.101 d	78.67±0.635 c	0.503±0.013 c
	4	43.57±2.101 b	79.70±0.635 bc	0.840±0.013 a
D	1	36.90±1.748 c	84.56±0.528 a	0.368±0.011 d
	2	30.41±1.748 d	81.64±0.528 b	0.581±0.011 c
	3	46.71±1.541 b	79.00±0.466 c	0.927±0.009 a
	4	60.33±1.748 a	79.05±0.528 c	0.659±0.011 b
E	1	66.61±1.748 a	92.22±0.528 a	1.191±0.011 a
	2	45.42±1.541 b	82.03±0.466 b	0.688±0.009 b
	3	40.31±1.748 c	76.87±0.528 c	0.423±0.011 c
	4	22.00±1.748 d	73.14±0.528 d	0.233±0.011 d

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