Effects of exogenous epigallocatechin gallate on resistance of melon seedlings to powdery mildew

doi:10.3969/j.issn.1004-1524.2023.01.15

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (1): 138-145.DOI: 10.3969/j.issn.1004-1524.2023.01.15

• Plant Protection • Previous Articles Next Articles

Effects of exogenous epigallocatechin gallate on resistance of melon seedlings to powdery mildew

FANG Mingya¹(), YU Hongwei², WU Yaxian¹, HAN Wenyan³, LI Xin³^,^*(), LIU Haihe¹^,^*()

1. College of Horticulture, Hebei Agricultural University, Baoding 071700, Hebei, China
2. Jiande Agricultural and Rural Bureau, Jiande 311600, Zhejiang, China
3. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

Received:2021-08-20 Online:2023-01-25 Published:2023-02-21

Abstract

Abstract:

Powdery mildew is a common fungal disease in melon production, which seriously affects the yield and quality of melon fruit. Effects of exogenous epigallocatechin gallate (EGCG) on the resistance of melon to powdery mildew was explored in this study. The results of diaminobenzidine (DAB) staining and malondialdehyde (MDA) determination showed that exogenous spraying with EGCG could reduce the accumulation of reactive oxygen species caused by powdery mildew infection, accompanied with the content of MDA decreased significantly, and the degree of cell membrane lipid peroxidation was decreased. The analysis of antioxidant enzyme activities in melon leaves showed that the activities of catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and peroxidase (POD) were increased significantly after exogenous EGCG pretreatment. Antioxidant enzymes activity was also induced by powdery mildew invasion, but exogenous EGCG pretreatment will further promote the improvement of antioxidant enzymes. The expression of related genes was further analyzed by real-time quantitative PCR. The results showed that the antioxidant enzyme related genes CmCAT, CmAPX, CmSOD and CmPOD were also induced by exogenous EGCG. The above results revealed that exogenous spraying EGCG could reduce the reactive oxygen species burst induced by powdery mildew infection by regulating the antioxidant enzymes system to improve the resistance of melon to powdery mildew.

Key words: melon, powdery mildew, epigallocatechin gallate, antioxidant enzymes

CLC Number:

S436.5

FANG Mingya, YU Hongwei, WU Yaxian, HAN Wenyan, LI Xin, LIU Haihe. Effects of exogenous epigallocatechin gallate on resistance of melon seedlings to powdery mildew[J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 138-145.

Figures/Tables 5

Table 1 Primers used for qRT-PCR

基因Gene	正向引物Forward primer (5'→3')	反向引物Reverse primer (5'→3')
CmCAT	TGGATACGACGATACAATGG	TGAGGACACTTAGGAGCAT
CmPOD	TTAGGACACCAGCAAGATT	TGAGAAGTGAGGACATTGAT
CmSOD	TCACGCTCACCAAGAAGACG	TTGGCAATTATGTTTCCCAGGTCACC
CmAPX	CTTCCAGATGCTCACCAA	TTGCGTATGCCTTCACAT
CmEF1a	ACTGTGCTGTCCTCATTATTG	AGGGTGAAAGCAAGAAGAGC

Fig.1 Effect of exogenous EGCG pretreatment on the phenotype of resistance in melon seedlings after powdery mildew infection

Fig.2 Effect of exogenous EGCG pretreatment on MDA content in melon seedlings after powdery mildew infection Different letters in the picture represented significant differences (P<0.05). The same as below.

Fig.3 Effects of exogenous EGCG pretreatment on antioxidant enzyme activities in melon seedlings after powdery mildew infection

Fig.4 Effects of exogenous EGCG pretreatment on the expression of antioxidant enzyme-related genes in melon seedlings after powdery mildew infection

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Effects of exogenous epigallocatechin gallate on resistance of melon seedlings to powdery mildew

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