Diversity evaluation of agronomic traits and construction of core collection and taste quality evaluation system in vegetable soybean germplasm resources

doi:10.3969/j.issn.1004-1524.2023.06.08

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (6): 1307-1314.DOI: 10.3969/j.issn.1004-1524.2023.06.08

• Horticultural Science • Previous Articles Next Articles

Diversity evaluation of agronomic traits and construction of core collection and taste quality evaluation system in vegetable soybean germplasm resources

BU Yuanpeng¹(), LIU Na¹, ZHANG Guwen¹, FENG Zhijuan¹, WANG Bin¹, GONG Yaming¹, XU Linying²^,^*()

1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Cixi Agricultural Technology Promotion Center, Cixi 315300, Zhejiang, China

Received:2022-10-31 Online:2023-06-25 Published:2023-07-04

Abstract

Abstract:

The identification and evaluation of germplasm resources is the first step to make full use of elite genes to accelerate the improvement of varieties. In order to explore the genetic diversity of agronomic traits and build an evaluation system for taste quality, 379 vegetable soybean lines widely collected throughout the country were adopted and 25 agronomic traits were investigated in 2 years. The diversity of this whole collection was analyzed and evaluated based on phenotypic data. The results showed that the 25 agronomic traits of the panel contained abundant genetic variation. Except for three quality traits, the diversity index of 22 quantitative traits varied from 1.40 to 2.06. Among the 8 methods of core gemplasm clustering, the core gemplasm constructed by the longest distance method was the most representative, covering all the traits variations of the original gemplasm. Seven key physical and chemical indexes affecting the taste quality of vegetable soybean and six sensory latitudes were determined in this core collection. A multiple linear regression model for vegetable soybean quality was constructed. It was clear that soluble total sugar, oil and total free amino acid contents were three key physical and chemical indexes affecting the taste quality. The three dimensions of sweetness, waxiness and hardness could explain 68.2% of the overall preference change rate. The results of this study are expected to provide a reference for improving the evaluation and utilization efficiency of vegetable soybean germplasm resources and accelerating the cultivation of high-yield and high-quality vegetable soybean varieties.

Key words: vegetable soybean, germplasm resource, agronomic trait, taste quality, diversity evaluation

CLC Number:

S643.7

BU Yuanpeng, LIU Na, ZHANG Guwen, FENG Zhijuan, WANG Bin, GONG Yaming, XU Linying. Diversity evaluation of agronomic traits and construction of core collection and taste quality evaluation system in vegetable soybean germplasm resources[J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1307-1314.

Figures/Tables 6

References 17

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类别 Types	性状 Traits	英文缩写 English abbreviation	变异幅度 Range	平均值±标准差 Mean±SD	多样性指数 H'	变异系数 CV/%
株型 Plant type	下胚轴颜色 Hypocotyl color	HypocotylC	—	—	0.52	—
	花色Flower color	FlowerC	—	—	0.51	—
	茸毛色Pubescence color	PubescenceC	—	—	0.29	—
	主茎节数Number of main stem nodes	NodeN	5.33~19.00	8.84±2.28	1.71	25.79
	有效分枝数Effective number of branches	BranchN	0.00~7.33	3.32±1.10	1.96	33.13
	株高Height/cm	PlantH	11.52~65.37	28.43±10.08	1.68	35.46
产量Yield	单株荚重Pod weight per plant/g	PodWP	24.14~234.78	97.04±30.7	2.01	31.64
	单株荚数Number of pods per plant	PodNP	13.67~233.67	46.16±24.56	1.45	53.21
	百粒鲜重Fresh weight of 100 grains/g	100SFW	16.80~122.50	73.16±21.65	2.04	29.59
	出仁率Kernel percent/%	KernelP	43.90~76.37	59.28±4.14	1.98	6.98
	10荚鲜重Fresh weight of 10 pods/g	10PFW	5.86~57.63	36.79±10.35	1.96	28.13
荚型Pod type	多粒荚比例Ratio of multiple pods	GraininessPR	0.11~0.75	0.39±0.15	2.06	38.46
	荚长Pod length/mm	PodL	35.81~73.27	58.5±6.58	1.95	11.25
	荚宽Pod width/mm	PodW	6.94~13.92	10.64±1.25	2.01	11.75
	荚长宽比Pod aspect ratio	PodAR	2.64~4.30	3.34±0.29	2.01	8.68
	荚平面面积Pod plane area/cm²	PodA	2.70~10.39	6.55±1.36	2.00	20.76
	弯曲夹角Bending angle/(°)	PodBA	129.62~177.96	170.57±7.1	1.40	4.16
	荚绿色强度Pod green intensity	PodGI	0.84~1.78	1.1±0.16	1.75	14.55
生育期	始花期Anthesis	BeginningF	33.50~54.00	39.85±4.22	1.89	10.59
Growth stages	鼓粒期Filling stage	FullS	66.00~109.00	78.54±7.79	1.89	9.92
	生殖生长期Reproductive stage	Reproductive	26.00~60.00	38.70±5.37	1.91	13.88
叶形Leaf type	叶长Leaf length/mm	LeafL	68.51~289.56	136.24±26.88	1.91	19.73
	叶宽Leaf width/mm	LeafW	30.45~115.07	77.00±12.55	1.97	16.30
	叶长宽比Leaf aspect ratio	LeafAR	1.24~3.85	1.80±0.40	1.65	22.22
	叶面积Leaf area/cm²	LeafA	16.23~198.15	72.42±24.76	1.93	34.19

类别 Types	性状 Traits	英文缩写 English abbreviation	变异幅度 Range	平均值±标准差 Mean±SD	多样性指数 H'	变异系数 CV/%
株型 Plant type	下胚轴颜色 Hypocotyl color	HypocotylC	—	—	0.52	—
	花色Flower color	FlowerC	—	—	0.51	—
	茸毛色Pubescence color	PubescenceC	—	—	0.29	—
	主茎节数Number of main stem nodes	NodeN	5.33~19.00	8.84±2.28	1.71	25.79
	有效分枝数Effective number of branches	BranchN	0.00~7.33	3.32±1.10	1.96	33.13
	株高Height/cm	PlantH	11.52~65.37	28.43±10.08	1.68	35.46
产量Yield	单株荚重Pod weight per plant/g	PodWP	24.14~234.78	97.04±30.7	2.01	31.64
	单株荚数Number of pods per plant	PodNP	13.67~233.67	46.16±24.56	1.45	53.21
	百粒鲜重Fresh weight of 100 grains/g	100SFW	16.80~122.50	73.16±21.65	2.04	29.59
	出仁率Kernel percent/%	KernelP	43.90~76.37	59.28±4.14	1.98	6.98
	10荚鲜重Fresh weight of 10 pods/g	10PFW	5.86~57.63	36.79±10.35	1.96	28.13
荚型Pod type	多粒荚比例Ratio of multiple pods	GraininessPR	0.11~0.75	0.39±0.15	2.06	38.46
	荚长Pod length/mm	PodL	35.81~73.27	58.5±6.58	1.95	11.25
	荚宽Pod width/mm	PodW	6.94~13.92	10.64±1.25	2.01	11.75
	荚长宽比Pod aspect ratio	PodAR	2.64~4.30	3.34±0.29	2.01	8.68
	荚平面面积Pod plane area/cm²	PodA	2.70~10.39	6.55±1.36	2.00	20.76
	弯曲夹角Bending angle/(°)	PodBA	129.62~177.96	170.57±7.1	1.40	4.16
	荚绿色强度Pod green intensity	PodGI	0.84~1.78	1.1±0.16	1.75	14.55
生育期	始花期Anthesis	BeginningF	33.50~54.00	39.85±4.22	1.89	10.59
Growth stages	鼓粒期Filling stage	FullS	66.00~109.00	78.54±7.79	1.89	9.92
	生殖生长期Reproductive stage	Reproductive	26.00~60.00	38.70±5.37	1.91	13.88
叶形Leaf type	叶长Leaf length/mm	LeafL	68.51~289.56	136.24±26.88	1.91	19.73
	叶宽Leaf width/mm	LeafW	30.45~115.07	77.00±12.55	1.97	16.30
	叶长宽比Leaf aspect ratio	LeafAR	1.24~3.85	1.80±0.40	1.65	22.22
	叶面积Leaf area/cm²	LeafA	16.23~198.15	72.42±24.76	1.93	34.19

聚类方法 Cluster method	MD	VD	CR	VR
可变法Flexible-beta method	0	60	100	116.86
离差平方和法Ward’s method	4	68	100	117.77
最长距离法Complete linkage	4	76	100	117.61
中间距离法Median method	12	64	100	117.55
类平均法Average linkage	16	76	100	120.25
可变类平均法	20	72	100	118.79
Flexible group average method
重心法Centroid method	56	88	100	122.56
最短距离法Single linkage	64	88	100	124.14

聚类方法 Cluster method	MD	VD	CR	VR
可变法Flexible-beta method	0	60	100	116.86
离差平方和法Ward’s method	4	68	100	117.77
最长距离法Complete linkage	4	76	100	117.61
中间距离法Median method	12	64	100	117.55
类平均法Average linkage	16	76	100	120.25
可变类平均法	20	72	100	118.79
Flexible group average method
重心法Centroid method	56	88	100	122.56
最短距离法Single linkage	64	88	100	124.14

性状 Traits	均值Mean		P_t	方差Variance		P_F	极差Range		变异系数CV/%
性状 Traits	原始种质 Original germplasm	核心种质 Core germplasm	P_t	原始种质 Original germplasm	核心种质 Core germplasm	P_F	原始种质 Original germplasm	核心种质 Core germplasm	原始种质 Original germplasm	核心种质 Core germplasm
主茎节数	8.84	9.45	0.04^*	5.23	8.38	<0.01^**	13.67	13.67	0.26	0.31
Number of main stem nodes
有效分枝数	3.32	3.24	0.56	1.22	1.87	<0.01^**	7.33	7.33	0.33	0.42
Effective number of branches
株高Height/cm	28.43	31.01	0.04^*	101.61	136.02	0.02^*	53.85	53.85	0.35	0.38
单株荚重	97.04	94.52	0.46	942.71	1 208.43	0.05^*	210.64	210.64	0.32	0.37
Pod weight per plant/g
单株荚数	46.16	51.06	0.14	604.65	1 042.08	<0.01^**	220.00	220.00	0.53	0.63
Number of pods per plant
百粒鲜重	73.16	76.73	0.22	469.80	616.87	0.03^*	105.70	105.70	0.30	0.37
Fresh weight of 100 grains/g
出仁率Kernel percent/%	59.28	59.02	0.62	17.14	26.14	<0.01^**	32.47	32.47	0.07	0.09
10荚鲜重	36.79	35.62	0.30	84.95	116.15	0.02^*	51.77	51.77	0.25	0.30
Fresh weight of 10 pods/g
多粒荚比例	0.39	0.40	0.49	0.02	0.02	0.07	0.64	0.64	0.35	0.38
Ratio of multiple pods
荚长Pod length/mm	58.5	57.2	0.1	42.83	58.02	0.02^*	37.46	37.46	0.11	0.13
荚宽Pod width/mm	10.64	10.44	0.17	1.54	2.06	0.02^*	6.98	6.98	0.12	0.14
荚长宽比Pod aspect ratio	3.34	3.31	0.42	0.08	0.09	0.27	1.66	1.66	0.09	0.09
荚平面面积	6.55	6.32	0.15	1.82	2.51	0.01^*	7.69	7.69	0.21	0.25
Pod plane area/cm²
弯曲夹角Bending angle/(°)	170.57	169.48	0.25	49.84	87.02	<0.01^**	48.34	48.34	0.04	0.06
荚绿色强度	1.10	1.13	0.21	0.03	0.04	<0.01^**	0.95	0.95	0.14	0.17
Pod green intensity
始花期Anthesis	39.85	40.29	0.41	17.81	26.81	<0.01^**	20.50	20.50	0.11	0.13
鼓粒期Filling stage	78.54	79.65	0.26	60.66	91.25	<0.01^**	43.00	43.00	0.10	0.12
生殖生长期	38.70	39.36	0.31	28.81	40.34	0.01^*	34.00	34.00	0.14	0.16
Reproductive stage
叶长Leaf length/mm	136.24	140.02	0.27	676.80	1 106.78	<0.01^**	221.05	221.05	0.19	0.24
叶宽Leaf width/mm	77.00	76.28	0.65	147.48	236.04	<0.01^**	84.62	84.62	0.16	0.20
叶长宽比Leaf aspect ratio	1.80	1.89	0.08	0.15	0.25	<0.01^**	2.61	2.61	0.22	0.27
叶面积Leaf area/cm²	72.42	74.78	0.46	574.23	1 000.1	<0.01^**	181.92	181.92	0.33	0.42

Diversity evaluation of agronomic traits and construction of core collection and taste quality evaluation system in vegetable soybean germplasm resources

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