Comparative analysis of chromosome karyotype between male and female red claw crayfish (Cherax quadricarinatus)

doi:10.3969/j.issn.1004-1524.20221534

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (9): 2079-2089.DOI: 10.3969/j.issn.1004-1524.20221534

• Animal Science • Previous Articles Next Articles

Comparative analysis of chromosome karyotype between male and female red claw crayfish (Cherax quadricarinatus)

LIU Fangfang¹(), CHEN Honglin²^,^*(), LIU Feng², XU Xiaojun², HUANG Fuyong², LOU Bao², QIAN Haojie³

1. School of Fishery, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China
2. Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. Haining Honghai Breeding Co., Ltd., Haining 314400, Zhejiang, China

Received:2022-10-31 Online:2023-09-25 Published:2023-10-09

Abstract

Abstract:

In order to obtain accurate chromosome number, arm length and other data to assist chromosome level genome assembly of red claw crayfish (Cherax quadricarinatus), as well as to understand its cytogenetic characteristics, the karyotype differences between male and female red claw crayfish chromosomes were analyzed. The hepatopancreas and testis of red claw crayfish were used as materials to screen the best duration of colchicine. The chromosome specimens were prepared by low permeability and air drying methods. The mitotic phases were obtained from male and female hepatopancreas, and the meiosis phases were obtained from testis. The number of chromosomes of 36 female and male mitotic phases and the number of bivalents of 36 male meiotic phases were counted. The results showed that the highest frequency of the number of chromosomes was 200, which accounted for 39.3% and 50.0% in female and male red claw crayfish, respectively. And the highest frequency of the number of bivalents was 100, which accounted for 50%. The mitotic metaphase with clear chromosome morphology and identifiable centromere was selected for karyotype analysis. It was found that the results of karyotype grouping of female and male red claw crayfish chromosomes were consistent, and the karyotype formula was n=100=36(m)+33(sm)+14(st)+17(t). No heterotypic chromosome was found. The results of independent sample t-test showed that there was no significant difference in chromosome length and chromosome arm ratio of each type between male and female red claw cravfish (P>0.05). The present study compared the chromosome karyotype of female and male red claw crayfish for the first time, which enriched the basic research of red claw crayfish cytogenetics and the chromosome karyotype information of decapods, provided basic data for the genetic breeding of red claw crayfish and reliable reference data for its genome assembly at the chromosome level.

Key words: red claw crayfish (Cherax quadricarinatus), hepatopancreas, testis, chromosome, karyotype analysis

CLC Number:

S917

LIU Fangfang, CHEN Honglin, LIU Feng, XU Xiaojun, HUANG Fuyong, LOU Bao, QIAN Haojie. Comparative analysis of chromosome karyotype between male and female red claw crayfish (Cherax quadricarinatus)[J]. Acta Agriculturae Zhejiangensis, 2023, 35(9): 2079-2089.

Figures/Tables 9

References 27

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染色体序号 Chromosome No.	雄性Male				雌性Female
染色体序号 Chromosome No.	长度 Length/μm	相对长度 Relative length/%	臂比 Arm ratio	类型 Type	长度 Length/μm	相对长度 Relative length/%	臂比 Arm ratio	类型 Type
1	3.220	2.5	1.405	m	2.667	2.4	1.323	m
2	2.943	2.3	1.571	m	2.285	2.1	1.246	m
3	2.825	2.2	1.244	m	2.122	1.9	1.089	m
4	2.422	1.9	1.242	m	1.829	1.7	1.436	m
5	2.369	1.9	1.181	m	1.697	1.6	1.482	m
6	2.110	1.7	1.275	m	1.552	1.4	1.287	m
7	1.963	1.5	1.515	m	1.409	1.3	1.170	m
8	1.777	1.4	1.426	m	1.384	1.3	1.551	m
9	1.742	1.4	1.360	m	1.246	1.1	1.417	m
10	1.697	1.3	1.458	m	1.224	1.1	1.150	m
11	1.605	1.3	1.117	m	1.187	1.1	1.194	m
12	1.423	1.1	1.220	m	1.135	1.0	1.368	m
13	1.357	1.1	1.188	m	1.121	1.0	1.448	m
14	1.279	1.0	1.417	m	1.096	1.0	1.260	m
15	1.252	1.0	1.273	m	1.017	0.9	1.351	m
16	1.251	1.0	1.133	m	0.997	0.9	1.382	m
17	1.155	0.9	1.465	m	0.997	0.9	1.304	m
18	1.133	0.9	1.343	m	0.985	0.9	1.227	m
19	1.126	0.9	1.362	m	0.982	0.9	1.496	m
20	1.090	0.9	1.497	m	0.940	0.9	1.406	m
21	1.046	0.8	1.557	m	0.920	0.8	1.172	m
22	1.043	0.8	1.211	m	0.908	0.8	1.168	m
23	1.008	0.8	1.167	m	0.899	0.8	1.064	m
24	0.963	0.8	1.534	m	0.890	0.8	1.262	m
25	0.928	0.7	1.442	m	0.871	0.8	1.639	m
26	0.897	0.7	1.492	m	0.864	0.8	1.280	m
27	0.893	0.7	1.453	m	0.836	0.8	1.389	m
28	0.892	0.7	1.382	m	0.829	0.8	1.052	m
29	0.789	0.6	1.415	m	0.825	0.8	1.156	m
30	0.788	0.6	1.516	m	0.812	0.7	1.122	m
31	0.768	0.6	1.489	m	0.710	0.6	1.320	m
32	0.731	0.6	1.270	m	0.656	0.6	1.209	m
33	0.676	0.5	1.404	m	0.647	0.6	1.247	m
34	0.634	0.5	1.158	m	0.643	0.6	1.197	m
35	0.537	0.4	1.498	m	0.599	0.5	1.192	m
36	0.418	0.3	1.596	m	0.573	0.5	1.234	m
37	3.652	2.9	2.963	sm	2.125	1.9	1.999	sm
38	3.223	2.5	2.535	sm	1.649	1.5	1.946	sm
39	3.189	2.5	2.407	sm	1.645	1.5	1.828	sm
40	2.148	1.7	2.284	sm	1.609	1.5	1.890	sm
41	1.986	1.6	1.867	sm	1.437	1.3	1.944	sm
42	1.711	1.3	2.169	sm	1.409	1.3	2.004	sm
43	1.589	1.2	1.972	sm	1.389	1.3	1.954	sm
44	1.373	1.1	2.272	sm	1.388	1.3	1.995	sm
45	1.338	1.0	2.521	sm	1.361	1.2	2.044	sm
46	1.214	1.0	1.949	sm	1.267	1.2	2.343	sm
47	1.211	0.9	2.068	sm	1.262	1.2	2.328	sm
48	1.201	0.9	1.935	sm	1.119	1.0	1.925	sm
49	1.189	0.9	2.175	sm	1.111	1.0	2.216	sm
50	1.183	0.9	1.874	sm	1.097	1.0	2.010	sm
51	1.167	0.9	1.745	sm	1.036	0.9	2.144	sm
52	1.127	0.9	1.934	sm	1.015	0.9	2.676	sm
53	1.090	0.9	2.481	sm	1.009	0.9	1.782	sm
54	1.089	0.9	1.835	sm	1.006	0.9	2.386	sm
55	1.085	0.8	1.750	sm	1.002	0.9	2.968	sm
56	1.069	0.8	1.854	sm	0.978	0.9	2.912	sm
57	1.039	0.8	2.485	sm	0.957	0.9	2.467	sm
58	0.987	0.8	1.848	sm	0.924	0.8	2.955	sm
59	0.940	0.7	1.918	sm	0.847	0.8	2.101	sm
60	0.920	0.7	1.997	sm	0.839	0.8	2.803	sm
61	0.888	0.7	2.024	sm	0.837	0.8	1.860	sm
62	0.885	0.7	1.968	sm	0.816	0.7	2.593	sm
63	0.860	0.7	1.976	sm	0.799	0.7	2.975	sm
64	0.860	0.7	1.709	sm	0.791	0.7	2.485	sm
65	0.859	0.7	1.784	sm	0.785	0.7	2.058	sm
66	0.756	0.6	1.940	sm	0.742	0.7	2.047	sm
67	0.732	0.6	1.846	sm	0.681	0.6	2.000	sm
68	0.671	0.5	1.947	sm	0.670	0.6	1.735	sm
69	0.573	0.4	1.974	sm	0.572	0.5	1.886	sm
70	2.531	2.0	3.496	st	2.471	2.3	3.284	st
71	2.169	1.7	3.508	st	2.066	1.9	3.480	st
72	1.863	1.5	4.628	st	1.372	1.3	3.042	st
73	1.775	1.4	3.498	st	1.174	1.1	3.103	st
74	1.676	1.3	4.710	st	1.123	1.0	3.806	st
75	1.492	1.2	4.007	st	1.065	1.0	5.507	st
76	1.396	1.1	3.274	st	1.019	0.9	3.687	st
77	1.252	1.0	3.052	st	1.016	0.9	3.231	st
78	1.024	0.8	3.621	st	1.001	0.9	3.488	st
79	1.017	0.8	3.468	st	0.991	0.9	4.730	st
80	0.979	0.8	3.418	st	0.946	0.9	3.645	st
81	0.916	0.7	3.024	st	0.858	0.8	3.108	st
82	0.915	0.7	3.463	st	0.815	0.7	4.461	st
83	0.814	0.6	3.229	st	0.628	0.6	5.009	st
84	2.025	1.6	∞	t	1.709	1.6	∞	t
85	1.488	1.2	8.727	t	1.473	1.3	∞	t
86	1.181	0.9	∞	t	1.238	1.1	∞	t
87	1.169	0.9	∞	t	1.151	1.1	∞	t
88	1.091	0.9	9.192	t	1.105	1.0	∞	t
89	1.008	0.8	∞	t	1.078	1.0	∞	t
90	0.921	0.7	∞	t	1.030	0.9	∞	t
91	0.915	0.7	∞	t	1.024	0.9	∞	t
92	0.891	0.7	∞	t	0.989	0.9	∞	t
93	0.863	0.7	∞	t	0.873	0.8	∞	t
94	0.863	0.7	∞	t	0.842	0.8	∞	t
95	0.701	0.5	∞	t	0.838	0.8	∞	t
96	0.613	0.5	∞	t	0.805	0.7	∞	t
97	0.529	0.4	∞	t	0.731	0.7	∞	t
98	0.459	0.4	∞	t	0.675	0.6	∞	t
99	0.436	0.3	∞	t	0.473	0.4	∞	t
100	0.161	0.1	∞	t	0.250	0.2	∞	t

染色体序号 Chromosome No.	雄性Male				雌性Female
染色体序号 Chromosome No.	长度 Length/μm	相对长度 Relative length/%	臂比 Arm ratio	类型 Type	长度 Length/μm	相对长度 Relative length/%	臂比 Arm ratio	类型 Type
1	3.220	2.5	1.405	m	2.667	2.4	1.323	m
2	2.943	2.3	1.571	m	2.285	2.1	1.246	m
3	2.825	2.2	1.244	m	2.122	1.9	1.089	m
4	2.422	1.9	1.242	m	1.829	1.7	1.436	m
5	2.369	1.9	1.181	m	1.697	1.6	1.482	m
6	2.110	1.7	1.275	m	1.552	1.4	1.287	m
7	1.963	1.5	1.515	m	1.409	1.3	1.170	m
8	1.777	1.4	1.426	m	1.384	1.3	1.551	m
9	1.742	1.4	1.360	m	1.246	1.1	1.417	m
10	1.697	1.3	1.458	m	1.224	1.1	1.150	m
11	1.605	1.3	1.117	m	1.187	1.1	1.194	m
12	1.423	1.1	1.220	m	1.135	1.0	1.368	m
13	1.357	1.1	1.188	m	1.121	1.0	1.448	m
14	1.279	1.0	1.417	m	1.096	1.0	1.260	m
15	1.252	1.0	1.273	m	1.017	0.9	1.351	m
16	1.251	1.0	1.133	m	0.997	0.9	1.382	m
17	1.155	0.9	1.465	m	0.997	0.9	1.304	m
18	1.133	0.9	1.343	m	0.985	0.9	1.227	m
19	1.126	0.9	1.362	m	0.982	0.9	1.496	m
20	1.090	0.9	1.497	m	0.940	0.9	1.406	m
21	1.046	0.8	1.557	m	0.920	0.8	1.172	m
22	1.043	0.8	1.211	m	0.908	0.8	1.168	m
23	1.008	0.8	1.167	m	0.899	0.8	1.064	m
24	0.963	0.8	1.534	m	0.890	0.8	1.262	m
25	0.928	0.7	1.442	m	0.871	0.8	1.639	m
26	0.897	0.7	1.492	m	0.864	0.8	1.280	m
27	0.893	0.7	1.453	m	0.836	0.8	1.389	m
28	0.892	0.7	1.382	m	0.829	0.8	1.052	m
29	0.789	0.6	1.415	m	0.825	0.8	1.156	m
30	0.788	0.6	1.516	m	0.812	0.7	1.122	m
31	0.768	0.6	1.489	m	0.710	0.6	1.320	m
32	0.731	0.6	1.270	m	0.656	0.6	1.209	m
33	0.676	0.5	1.404	m	0.647	0.6	1.247	m
34	0.634	0.5	1.158	m	0.643	0.6	1.197	m
35	0.537	0.4	1.498	m	0.599	0.5	1.192	m
36	0.418	0.3	1.596	m	0.573	0.5	1.234	m
37	3.652	2.9	2.963	sm	2.125	1.9	1.999	sm
38	3.223	2.5	2.535	sm	1.649	1.5	1.946	sm
39	3.189	2.5	2.407	sm	1.645	1.5	1.828	sm
40	2.148	1.7	2.284	sm	1.609	1.5	1.890	sm
41	1.986	1.6	1.867	sm	1.437	1.3	1.944	sm
42	1.711	1.3	2.169	sm	1.409	1.3	2.004	sm
43	1.589	1.2	1.972	sm	1.389	1.3	1.954	sm
44	1.373	1.1	2.272	sm	1.388	1.3	1.995	sm
45	1.338	1.0	2.521	sm	1.361	1.2	2.044	sm
46	1.214	1.0	1.949	sm	1.267	1.2	2.343	sm
47	1.211	0.9	2.068	sm	1.262	1.2	2.328	sm
48	1.201	0.9	1.935	sm	1.119	1.0	1.925	sm
49	1.189	0.9	2.175	sm	1.111	1.0	2.216	sm
50	1.183	0.9	1.874	sm	1.097	1.0	2.010	sm
51	1.167	0.9	1.745	sm	1.036	0.9	2.144	sm
52	1.127	0.9	1.934	sm	1.015	0.9	2.676	sm
53	1.090	0.9	2.481	sm	1.009	0.9	1.782	sm
54	1.089	0.9	1.835	sm	1.006	0.9	2.386	sm
55	1.085	0.8	1.750	sm	1.002	0.9	2.968	sm
56	1.069	0.8	1.854	sm	0.978	0.9	2.912	sm
57	1.039	0.8	2.485	sm	0.957	0.9	2.467	sm
58	0.987	0.8	1.848	sm	0.924	0.8	2.955	sm
59	0.940	0.7	1.918	sm	0.847	0.8	2.101	sm
60	0.920	0.7	1.997	sm	0.839	0.8	2.803	sm
61	0.888	0.7	2.024	sm	0.837	0.8	1.860	sm
62	0.885	0.7	1.968	sm	0.816	0.7	2.593	sm
63	0.860	0.7	1.976	sm	0.799	0.7	2.975	sm
64	0.860	0.7	1.709	sm	0.791	0.7	2.485	sm
65	0.859	0.7	1.784	sm	0.785	0.7	2.058	sm
66	0.756	0.6	1.940	sm	0.742	0.7	2.047	sm
67	0.732	0.6	1.846	sm	0.681	0.6	2.000	sm
68	0.671	0.5	1.947	sm	0.670	0.6	1.735	sm
69	0.573	0.4	1.974	sm	0.572	0.5	1.886	sm
70	2.531	2.0	3.496	st	2.471	2.3	3.284	st
71	2.169	1.7	3.508	st	2.066	1.9	3.480	st
72	1.863	1.5	4.628	st	1.372	1.3	3.042	st
73	1.775	1.4	3.498	st	1.174	1.1	3.103	st
74	1.676	1.3	4.710	st	1.123	1.0	3.806	st
75	1.492	1.2	4.007	st	1.065	1.0	5.507	st
76	1.396	1.1	3.274	st	1.019	0.9	3.687	st
77	1.252	1.0	3.052	st	1.016	0.9	3.231	st
78	1.024	0.8	3.621	st	1.001	0.9	3.488	st
79	1.017	0.8	3.468	st	0.991	0.9	4.730	st
80	0.979	0.8	3.418	st	0.946	0.9	3.645	st
81	0.916	0.7	3.024	st	0.858	0.8	3.108	st
82	0.915	0.7	3.463	st	0.815	0.7	4.461	st
83	0.814	0.6	3.229	st	0.628	0.6	5.009	st
84	2.025	1.6	∞	t	1.709	1.6	∞	t
85	1.488	1.2	8.727	t	1.473	1.3	∞	t
86	1.181	0.9	∞	t	1.238	1.1	∞	t
87	1.169	0.9	∞	t	1.151	1.1	∞	t
88	1.091	0.9	9.192	t	1.105	1.0	∞	t
89	1.008	0.8	∞	t	1.078	1.0	∞	t
90	0.921	0.7	∞	t	1.030	0.9	∞	t
91	0.915	0.7	∞	t	1.024	0.9	∞	t
92	0.891	0.7	∞	t	0.989	0.9	∞	t
93	0.863	0.7	∞	t	0.873	0.8	∞	t
94	0.863	0.7	∞	t	0.842	0.8	∞	t
95	0.701	0.5	∞	t	0.838	0.8	∞	t
96	0.613	0.5	∞	t	0.805	0.7	∞	t
97	0.529	0.4	∞	t	0.731	0.7	∞	t
98	0.459	0.4	∞	t	0.675	0.6	∞	t
99	0.436	0.3	∞	t	0.473	0.4	∞	t
100	0.161	0.1	∞	t	0.250	0.2	∞	t

Comparative analysis of chromosome karyotype between male and female red claw crayfish (Cherax quadricarinatus)

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