Development and validation of molecular sex identification method in Pelodiscus sinensis

doi:10.3969/j.issn.1004-1524.20230385

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (11): 2594-2599.DOI: 10.3969/j.issn.1004-1524.20230385

• Animal Science • Previous Articles Next Articles

Development and validation of molecular sex identification method in Pelodiscus sinensis

GE Jianhui¹^,²(), REN Jindong¹, GUAN Wenzhi¹, NIU Baolong¹, WANG Wei², LOU Bao¹, YU Jin³^,^*(), XU Xiaojun¹^,^*()

1. Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China
3. Longyou County Breeding Development Center, Quzhou 324000, Zhejiang, China

Received:2023-03-22 Online:2023-11-25 Published:2023-12-04

Abstract

Abstract:

In order to develop a molecular sex identification method for Pelodiscus sinensis, an intron length difference of SBNO1 gene was screened by analyzing and comparing the sequences of two copies of SBNO1 gene in female P. sinensis genome. And primers design, PCR verification, sequencing were performed. The accuracy of this sex identification method was further tested with 48 P. sinensis individuals (24 male and 24 female respectively). The results showed that the amplification products of the female individual contained 2 bands (2 031 bp and 1 624 bp), and that of the male individual had a single band (2 031 bp). Combined with anatomical sex identification records, the accuracy of sex identification was 100%. This study provided a minimally invasive, simple and fast molecular sex identification method for P. sinensis, which laid a foundation for the monosex cultivation and sex controlled breeding.

Key words: Pelodiscus sinensis, molecular sex identification, polymerase chain reaction

CLC Number:

S917.4

GE Jianhui, REN Jindong, GUAN Wenzhi, NIU Baolong, WANG Wei, LOU Bao, YU Jin, XU Xiaojun. Development and validation of molecular sex identification method in Pelodiscus sinensis[J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2594-2599.

Figures/Tables 2

References 27

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Development and validation of molecular sex identification method in Pelodiscus sinensis

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